Marc Phillipe Yves Desmulliez

Lab-on-a-chip based immunosensor principles and technologies for the detection of cardiac biomarkers: a review

This review examines the current state of the art lab-on-a-chip and microfluidic based biosensor technologies used in the detection of cardiac biomarkers. The determination and quantification of blood based, cardiac biomarkers are crucial in the triage and management of a range of cardiac related conditions, where time delay has a major impact on short and longer-term outcomes of a patient. The design and manufacturing of biomarker detection systems are multi-disciplinary in nature and require researchers to have knowledge of both life sciences and engineering for the full potential of this field to be realised. This review will therefore provide a comprehensive overview of chip based immunosensing technology as applied to cardiac biomarker detection, while discussing the potential suitability and limitations of each configuration for incorporation within a clinical diagnostics device suitable for point-of-care applications.

Inkjet printing of conductive materials: a review

Purpose – The purpose of this paper is to present an exhaustive review of research studies and activities in the inkjet printing of conductive materials.Design/methodology/approach – This paper gives a detailed literature survey of research carried out in inkjet printing of conductive materials.Findings – This article explains the inkjet printing process and the various types of conductive inks. It then examines the various factors that affect the quality of inkjet printed interconnects such as printing parameters, materials and substrate treatments. Methods of characterising both the inkjet printing process and the electrical properties of printed conductive materials are also presented. Finally relevant applications of this technology are described.Originality/value – Inkjet printing is currently one of the cheapest direct write techniques for manufacturing. The use of this technique in electronic manufacturing, where interconnects and other conductive features are required is an area of increasing rele...

Recent advances in microparticle continuous separation

Recent advances in microparticle separation in continuous flow are presented. It is intended for scientists in the field of separation science in biology, chemistry and microsystems engineering. Recent techniques of micron-sized particle separation within microsystems are described with emphasis on five different categories: optical, magnetic, fluidic-only, electrical and minor separation methods. Examples from the growing literature are explained with insights on separation efficiency and microengineering challenges. Current applications of the techniques are discussed.

Architectural approach to the role of Optics in monoprocessor and multiprocessor machines

The relevance of introducing optical interconnects (OIs) in monoprocessors and multiprocessors is studied from an architectural point of view. We show that perhaps the major explanation for why optical technologies have nearly been unable to penetrate into computers is that OIs generally do not shorten the memory-access time, which is the most critical issue for todays stored-program machines. In monoprocessors the memory-access time is dominated by the electronic latency of the memory itself. Thus implementing OIs inside the memory hierarchy without changing the memory architecture cannot dramatically improve the global performance. In strongly coupled multiprocessors the node-bypass latency dominates. Therefore the higher the connectivity (possibly with optics), the shorter the path to another node, but the more expensive the network and the more complex the structure of electronic nodes. This relation leaves the choice of the best network open in terms of simplicity and latency reduction. The bottlenecks resulting from and the benefits of implementing OIs are discussed with respect to symmetric multiprocessors, rings, and distributed shared-memory supercomputers.

Validation of a blood plasma separation system by biomarker detection

A microfluidic system was developed for blood plasma separation at high flow rate. This system uses only hydrodynamic forces to separate plasma from whole blood. The microfluidic network features a series of constrictions and bifurcations to enhance the product yield and purity. A maximum purity efficiency of 100% is obtained on blood with entrance hematocrit level up to 30% with a flow rate of 2 mL h(-1). Flow cytometry was performed on the extracted plasma to evaluate the separation efficiency and to assess cell damage. A core target of this study was the detection of cell-free DNA from the on-chip extracted plasma. To this effect, PCR was successfully carried out off-chip on the cell-free DNA present in the plasma extracted on-chip. A house-keeping gene sequence (GAPDH) was amplified without the need for a purification after the separation, thereby showing the high quality of the plasma sample. The resulting data suggests that the system can be used as a preliminary module of a total analysis system for cell-free DNA detection in human plasma.

Detection of Cryptosporidium in miniaturised fluidic devices

Contamination of drinking water with the protozoan pathogen, Cryptosporidium, represents a serious risk to human health due to the low infectious dose and the resistance of this parasite to chlorine disinfection. Therefore, several countries have legislated for the frequent monitoring of drinking water for Cryptosporidium presence. Existing approved monitoring protocols are however time-consuming and do not provide essential information on the species, virulence or viability of detected oocysts. Rapid, more information-rich and automatable systems for Cryptosporidium detection are highly sought-after, and numerous miniaturised devices have been developed to address this need. This review article aims to summarise the state-of-the-art and compare the performance of these systems in terms of detection limit, ability to determine species, viability and performance in the presence of interferents. Finally, conclusions are drawn with regard to the most promising methods and directions of future research.

Application of microfluidics in waterborne pathogen monitoring: A review

A review of the recent advances in microfluidics based systems for the monitoring of waterborne pathogens is provided in this article. Emphasis has been made on existing, commercial and state-of-the-art systems and research activities in laboratories worldwide. The review separates sample processing systems and monitoring systems, highlighting the slow progress made in automated sample processing for monitoring of pathogens in waterworks and in the field. Future potential directions of research are also highlighted in the conclusions.

Design and construction of an optoelectronic crossbar switch containing a terabit per second free-space optical interconnect

The completed detailed design and initial phases of construction of an optoelectronic crossbar demonstrator are presented. The experimental system uses hybrid very large scale integrated optoelectronics technology whereby InGaAs-based detectors and modulators are flip-chip bonded onto silicon integrated circuits. The system aims to demonstrate a 1-Tb/s aggregate data input/output to a single chip by means of free-space optics.

Factors affecting user performance in haptic assembly

Current computer-aided assembly systems provide engineers with a variety of spatial snapping and alignment techniques for interactively defining the positions and attachments of components. With the advent of haptics and its integration into virtual assembly systems, users now have the potential advantage of tactile information. This paper reports research that aims to quantify how the provision of haptic feedback in an assembly system can affect user performance. To investigate human–computer interaction processes in assembly modeling, performance of a peg-in-hole manipulation was studied to determine the extent to which haptics and stereovision may impact on task completion time. The results support two important conclusions: first, it is apparent that small (i.e. visually insignificant) assembly features (e.g. chamfers) affect the overall task completion at times only when haptic feedback is provided; and second, that the difference is approximately similar to the values reported for equivalent real world peg-in-hole assembly tasks.

Autonomous capillary microfluidic system with embedded optics for improved troponin I cardiac biomarker detection

Cardiovascular diseases are the most prevalent medical conditions affecting the modern world, reducing the quality of life for those affected and causing an ever increasing burden on clinical resources. Cardiac biomarkers are crucial in the diagnosis and management of patient outcomes. In that respect, such proteins are desirable to be measured at the point of care, overcoming the shortcomings of current instrumentation. We present a CO2 laser engraving technique for the rapid prototyping of a polymeric autonomous capillary system with embedded on-chip planar lenses and biosensing elements, the first step towards a fully miniaturised and integrated cardiac biosensing platform. The system has been applied to the detection of cardiac Troponin I, the gold standard biomarker for the diagnosis of acute myocardial infarction. The devised lab-on-a-chip device was demonstrated to have 24 pg/ml limit of detection, which is well within the minimum threshold for clinically applicable concentrations. Assays were completed within approximately 7-9 min. Initial results suggest that, given the portability, low power consumption and high sensitivity of the device, this technology could be developed further into point of care instrumentation useful in the diagnosis of various forms of cardiovascular diseases.