Marc Ylieff

Comparing the EthoVision 2.3 system and a new computerized multitracking prototype system to measure the swimming behavior in fry fish

Coming from the framework of unmarked fry tracking, we compared the capacities, advantages, and disadvantages of two recent video tracking systems: EthoVision 2.3 and a new prototype of multitracking. The EthoVision system has proved to be impressive for tracking a fry using the detection by gray scaling. Detection by subtraction has given less accurate results. Our video multitracking system is able to detect and track more than 100 unmarked fish by gray scaling technique. It permits an analysis at the group level as well as at the individual level. The multitracking program is able to attribute a number to each fish and to follow each one for the whole duration of the track. Our system permits the analysis of the movement of each individual, even if the trajectories of two fish cross each other. This is possible thanks to the theoretical estimation of the trajectory of each fish, which can be compared with the real trajectory (analysis with feedback). However, the period of the track is limited for our system (about 1 min), whereas EthoVision is able to track for numerous hours. In spite of these limitations, these two systems allow an almost continuous automatic sampling of the movement behaviors during the track.

Video tracking in the extreme: A new possibility for tracking nocturnal underwater transparent animals with fluorescent elastomer tags

Initially developed so that an individual could be recognized in mark–recapture studies of aquatic animals, fluorescent visible implant elastomer (VIE) tags are used here for a new application in ethometry: the study of the behavior of transparent animals in dim light or in darkness using automatic tracking technology. The application and validation of this multitracking method is tested in the context of research on the estuarine migratory behavior of the glass eel (Anguilla anguilla), a crucial point to better understand the dynamics of this endangered species. The method makes it possible to measure the activity (notably the distance and speed) of four individuals as a function of tidal and nycthemeral rhythms in the same flume (a circular aquarium simulating river or estuarine conditions) across a wide time scale (from seconds to weeks).

Quantifying spontaneous swimming activity in fish with a computerized color video tracking system, a laboratory device using last imaging techniques

Thanks to computing power offered by digital imaging techniques, laboratory studies on fish have now the capacity to detect and to quantify behavioral parameters that usually cannot be measured by direct observation. After adjusting to fish studies the EthoVision system, a commercial (Noldus Information Technology) computerized video tracking device, we conducted experiments in Chromis chromis (Mediterranean marine fish) and in Carassius auratus. Since temperature is almost certainly one of the most important abiotic environmental effectors influencing reproduction, we tested under laboratory conditions its effects on behavioral patterns of free-swimming fish.