Márcia Duarte Adorne
Universidade Federal do Rio Grande do Sul
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Publication
Featured researches published by Márcia Duarte Adorne.
International Journal of Pharmaceutics | 2012
Eduardo André Bender; Márcia Duarte Adorne; Letícia Marques Colomé; Dulcineia S.P. Abdalla; Silvia Stanisçuaski Guterres; Adriana Raffin Pohlmann
The hemocompatibility of nanoparticles is of critical importance for their systemic administration as drug delivery systems. Formulations of lipid-core nanocapsules, stabilized with polysorbate 80-lecithin and uncoated or coated with chitosan (LNC and LNC-CS), were prepared and characterized by laser diffraction (D[4,3]: 129 and 134 nm), dynamic light scattering (119 nm and 133 nm), nanoparticle tracking (D50: 124 and 139 nm) and particle mobility (zeta potential: -15.1 mV and +9.3 mV) analysis. In vitro hemocompatibility studies were carried out with mixtures of nanocapsule suspensions in human blood at 2% and 10% (v/v). The prothrombin time showed no significant change independently of the nanocapsule surface potential or its concentration in plasma. Regarding the activated partial thromboplastin time, both suspensions at 2% (v/v) in plasma did not influence the clotting time. Even though suspensions at 10% (v/v) in plasma decreased the clotting times (p<0.05), the values were within the normal range. The ability of plasma to activate the coagulation system was maintained after the addition of the formulations. Suspensions at 2% (v/v) in blood showed no significant hemolysis or platelet aggregation. In conclusion, the lipid-core nanocapsules uncoated or coated with chitosan are hemocompatible representing a potential innovative nanotechnological formulation for intravenous administration.
Soft Matter | 2011
Luana Almeida Fiel; Luciana Magalhães Rebêlo; Thiago de Melo Santiago; Márcia Duarte Adorne; Silvia Stanisçuaski Guterres; Jeanlex Soares de Sousa; Adriana Raffin Pohlmann
The deformation properties of submicrometric drug carriers can influence their tissue-penetration ability and thus the drug targeting. The aim of this study was to determine whether the oily core composition (raw oils or a dispersion of oils and solid lipid) surrounded by a polymeric wall [poly(e-caprolactone), (PCL)] can affect the deformation properties of nanocapsules (NCs) or lipid-core nanocapsules (LNCs). Formulations were prepared as aqueous suspensions using a polymer and either a mixture of caprylic/capric triglyceride (CCT) and octyl methoxycinnamate (OMC) or a mixture of CCT, OMC and sorbitan monostearate (SM) as core components, respectively. Formulations had mean diameters close to 200 nm presenting monomodal distributions. A polysorbate 80 coating rendered ζ-potential values close to zero, acting as a steric stabilizer. Atomic force microscopy (AFM) showed, through force curves analysis, that the cantilever deflection was more pronounced for the LNCs than for the NCs. The same force applied to NC produced an indentation around twice that observed for the LNCs. The Youngs modulus (E) values were 0.537 MPa (LNC) and 0.364 MPa (NC) considering conical geometry while E = 0.17 MPa (NC) and E = 0.241 (LNC) for spherical geometry. These data confirm that the LNCs are stiffer than the NCs. The rigidity of both the polymer wall and lipid core is higher for LNCs. In conclusion, LNCs presented distinct mechanical properties compared to the conventional polymeric NCs.
Current Pharmaceutical Design | 2013
Kelly Silveira da Silva Bueno; Márcia Duarte Adorne; Denise Soledade Jornada; Francisco N. Fonseca; Silvia Stanisçuaski Guterres; Adriana Raffin Pohlmann
In nanomedicine, different nanomaterials and nanoparticles have been proposed as therapeutic agents or adjuvants, as well as diagnosis devices. Considering that the principal cause of the ulcerations is the imbalance among the gastric juice secretion and the protection provided by the mucosal barrier and the neutralization of the gastric acid, as well as that nanoparticles are able to accumulate in the gastro-intestinal tissues, we proposed a 2(2) factorial design to evaluate the influence of the chemical composition and the volume fraction of the dispersed phase on the gastric protective effect against ulceration induced by ethanol. Cocoa-theospheres (CT) and lipid-core nanocapsules (LNC) (two different kinds of surfaces: lipid and polymeric, respectively) prepared at two different concentrations of soft materials: 4% and 12% (w/v) were produced by high pressure homogenization and solvent displacement methods, respectively. Laser diffraction showed volume-weighted mean diameters ranging from 133 to 207 nm, number median diameters lower than 100 nm and specific surfaces between 41.2 and 51.2 m(2) g(-1). The formulations had pH ranging from 4.7 to 6.3; and zeta potential close to -9 mV due to their coating with polysorbate 80. The ulcer indexes were 0.40 (LNC(4)) and 0.48 (CT(4)) for the lower total administered areas (3.3 and 4.1 m(2)g(-1), respectively), and 0.09 (LNC(12) and CT(12)) for the higher administered areas (10.0 and 12.0 m(2) g(-1), respectively). LNC(4), LNC(12) and CT(12) showed lower levels in the lipid peroxidation assay when compared either to the negative control (saline) or to CT(4). LNC(12) and CT(12) showed similar TBARS levels, as well as CT(4) was similar to the negative control. SEM analysis of the stomach mucosa showed coatings more homogenous and cohesive when LNC formulations were administered compared to the correspondent CT formulations. The higher total area of administered nanoparticles showed film formation. Moreover, LNC(12) provided a more thick and cohesive film, completely covering the mucosal surface. In conclusion, both kinds of formulations are able to prevent ulceration induced by ethanol in rats. The 2(2) factorial design showed that the chemical composition had a strong influence when the lower areas of nanoparticles are administered, while when the higher areas are used this is the more influencing parameter on the gastroprotection.
European Journal of Pharmaceutics and Biopharmaceutics | 2016
Marcela Frota Cavalcante; Soraya Megumi Kazuma; Eduardo André Bender; Márcia Duarte Adorne; Mayara Ullian; Mariana Matera Veras; Paulo Hilário Nascimento Saldiva; Andrea Queiroz Maranhão; Silvia Stanisçuaski Guterres; Adriana Raffin Pohlmann; Dulcineia Saes Parra Abdalla
Atherosclerosis is a chronic inflammatory disease responsible for the majority of cases of myocardial infarction and ischemic stroke. The electronegative low-density lipoprotein, a modified subfraction of native LDL, is pro-inflammatory and plays an important role in atherogenesis. To investigate the effects of a nanoformulation (scFv anti-LDL(-)-MCMN-Zn) containing a scFv reactive to LDL(-) on the inhibition of atherosclerosis, its toxicity was evaluated in vitro and in vivo and further it was also administered weekly to LDL receptor knockout mice. The scFv anti-LDL(-)-MCMN-Zn nanoformulation did not induce cell death in RAW 264.7 macrophages and HUVECs. The 5mg/kg dose of scFv anti-LDL(-)-MCMN-Zn did not cause any typical signs of toxicity and it was chosen for the evaluation of its atheroprotective effect in Ldlr(-/-) mice. This nanoformulation significantly decreased the atherosclerotic lesion area at the aortic sinus, compared with that in untreated mice. In addition, the Il1b mRNA expression and CD14 protein expression were downregulated in the atherosclerotic lesions at the aortic arch of Ldlr(-/-) mice treated with scFv anti-LDL(-)-MCMN-Zn. Thus, the scFv anti-LDL(-)-MCMN-Zn nanoformulation inhibited the progression of atherosclerotic lesions, indicating its potential use in a future therapeutic strategy for atherosclerosis.
Colloids and Surfaces A: Physicochemical and Engineering Aspects | 2013
Luana Almeida Fiel; Márcia Duarte Adorne; Silvia Stanisçuaski Guterres; Paulo A. Netz; Adriana Raffin Pohlmann
Pharmaceutical Research | 2015
Fabiana Quoos Mayer; Márcia Duarte Adorne; Eduardo André Bender; Talita Giacomet de Carvalho; Anna Cláudia Dilda; Ruy Carlos Ruver Beck; Silvia Stanisçuaski Guterres; Roberto Giugliani; Ursula da Silveira Matte; Adriana Raffin Pohlmann
Pharmaceutical Research | 2014
Eduardo André Bender; Marcela Frota Cavalcante; Márcia Duarte Adorne; Letícia Marques Colomé; Silvia Stanisçuaski Guterres; Dulcineia S.P. Abdalla; Adriana Raffin Pohlmann
Atherosclerosis | 2017
Dulcineia S.P. Abdalla; Marcela Frota Cavalcante; Walter M. Turato; Soraya Megumi Kazuma; Márcia Duarte Adorne; Silvia Stanisçuaski Guterres; Adriana Raffin Pohlmann
Archive | 2013
Márcia Duarte Adorne; Sabrina Laíz Büttenbender; Silvia Stanisçuaski Guterres; Adriana Raffin Pohlmann
Archive | 2012
Dulcineia Saes Parra Abdalla; Andrea Queiroz Maranhão; Adriana Raffin Pohlmann; Silvia Stanisçuaski Guterres; Eduardo André Bender; Marcela Frota Cavalcante; Márcia Duarte Adorne; Soraya Megumi Kazuma