Marcio Martinello Sanches

Levantamento revela a predominância do Lettuce mottle virus em três regiões produtoras de alface no Estado de São Paulo

ABSTRACT A alface e infectada por diversos virus que causam sintomas demosaico, distorcao foliar e necrose resultando em perda na qualidadedo produto. Dentre esses virus destacam-se o Lettuce mosaic virus ,LMV pertencente ao genero Potyvirus, considerado o maisimportante na cultura (4), o Lettuce mottle virus, LeMoV possivelmembro do genero Sequivirus e os virus do genero Tospovirus ,causadores da doenca conhecida como vira-cabeca. Alem destes, oBidens mosaic virus (BiMV), especie tentativa no genero Potyvirus ,e o Cucumber mosaic virus (CMV, Cucumovirus ) tambem infectama alface (6).Dispondo de oligonucleotideos sensiveis e especificos para oLMV, LeMoV e BiMV, todos causadores de sintomas de mosaicomuito similares, o objetivo deste trabalho foi de avaliar a ocorrenciado LMV, LeMoV e do BiMV, nos campos de producao de alfacede tres regioes do estado de Sao Paulo.Foram coletadas amostras de alface e plantas invasoras comopicao-preto ( Bidens pilosa ) e fazendeiro ( Galinsoga parviflora

Development of methodologies for virus detection in soybean and wheat seeds

Graphical abstract

Interception of ‘Candidatus Phytoplasma solani’ in Brazil in apple tree propagative material imported from France

Arq. Inst. Biol., v.84, 1-4, e0602016, 2017 ABSTRACT: Apple plants from France introduced in Brazil for research purposes were subjected to a phytosanitary analysis at the Plant Quarantine Laboratory of Embrapa Genetic Resources and Biotechnology (Cenargen). After grafting onto healthy apple rootstock, some plants showed phytoplasmainfection symptoms. Polymerase Chain Reaction (PCR) tests yielded DNA fragments of the expected size for phytoplasmas. DNA sequencing revealed an identity of the 16S rDNA nucleotide sequence of 98-99% with ‘Candidatus Phytoplasma solani’. This phytoplasma species is responsible for losses in European apple orchards and has not been reported in Brazil. According to the Federal Legislation on Plant Protection, the plants were incinerated to avoid the introduction of this exotic pest in Brazil.

Interceptação do Wheat mosaic virus (WMoV) no Brasil em sementes de milho provenientes dos Estados Unidos

The objective of this work was to evaluate the phytosanitary aspect of two accessions of maize ( Zea mays ) seeds from the United States introduced to Brazil, regarding the presence of Wheat mosaic virus (WMoV). Two to three weeks after sowing, symptomatic leaves were tested by Elisa using specific antiserum to WMoV. The reaction was positive, and leaf samples were analyzed by real‑time PCR and amplified PCR products were sequenced. The WMoV isolates had 99 to 100% nucleotide identity with isolates from Australia and the United States. Until now, there is no report of the presence of this virus in Brazil. According to the federal law on plant protection, the plants were burned to avoid the introduction of this exotic pest in the country. The obtained results show WMoV interception in Brazil.