Marco Volterrani

Unmanned Aerial Vehicle to Estimate Nitrogen Status of Turfgrasses

Spectral reflectance data originating from Unmanned Aerial Vehicle (UAV) imagery is a valuable tool to monitor plant nutrition, reduce nitrogen (N) application to real needs, thus producing both economic and environmental benefits. The objectives of the trial were i) to compare the spectral reflectance of 3 turfgrasses acquired via UAV and by a ground-based instrument; ii) to test the sensitivity of the 2 data acquisition sources in detecting induced variation in N levels. N application gradients from 0 to 250 kg ha-1 were created on 3 different turfgrass species: Cynodon dactylon x transvaalensis (Cdxt) ‘Patriot’, Zoysia matrella (Zm) ‘Zeon’ and Paspalum vaginatum (Pv) ‘Salam’. Proximity and remote-sensed reflectance measurements were acquired using a GreenSeeker handheld crop sensor and a UAV with onboard a multispectral sensor, to determine Normalized Difference Vegetation Index (NDVI). Proximity-sensed NDVI is highly correlated with data acquired from UAV with r values ranging from 0.83 (Zm) to 0.97 (Cdxt). Relating NDVI-UAV with clippings N, the highest r is for Cdxt (0.95). The most reactive species to N fertilization is Cdxt with a clippings N% ranging from 1.2% to 4.1%. UAV imagery can adequately assess the N status of turfgrasses and its spatial variability within a species, so for large areas, such as golf courses, sod farms or race courses, UAV acquired data can optimize turf management. For relatively small green areas, a hand-held crop sensor can be a less expensive and more practical option.

N source affects freeze tolerance in bermudagrass and zoysiagrass

Abstract During cold acclimation, warm-season grasses exhibit growth stall and eventually the onset of rest. Late-season N fertilization is an alternative way for reduction of the dormancy period. The objectives of the present study were (1) to assess the effects of different N sources, and timing on freezing tolerance using controlled environment acclimation and freezing procedures and (2) to determine the relationship of nonstructural carbohydrates and photosynthetic pigments with the freeze tolerance of bermudagrass and zoysiagrass. source treatment exhibited a relative peak at 14 days (2 weeks) before the treatment. In bermudagrass, treatment depressed starch accumulation at 14 and 28 days compared to the control. As expected, in both species a higher concentration of soluble carbohydrates were observed in sprigs tissues. A sharp decline was evident on the treatment in leaves, stems, and roots tissues at 14 days, in correspondence with a lower freeze tolerance, whereas in the treatment an accumulation in total soluble sugars was observed in rhizomes at 28 days. Over the time, chlorophylls showed a slight increase in the treatment, while an opposite trend was detected in the treatment. Overall, N source provided consistent differences in freeze tolerance in both species.

Physiological and morphological factors influencing wear resistance and recovery in C3 and C4 turfgrass species

High turfgrass wear resistance and recovery are the most sought after characteristics in turfgrass species when used for sports turf, but they are also very important in home gardens and public parks. Several wear resistance investigations have been conducted in field conditions in recent years, but these investigations involved the use of machinery and techniques that are not able to segregate the plant wounding and death effects from soil compaction effects that are generally associated with turfgrass wear. The same can be said of wear recovery investigations, with an extensive use of agronomical machinery for field trials. This study focussed on the wear resistance and recovery of mature swards of Cynodon dactylon (L.) Pers. var. dactylon×C. transvaalensis Burt-Davy cv. Tifway 419, Zoysia matrella (L.) Merr. cv. Zeon and Paspalum vaginatum Swartz. cv. Salam and a typical sports-type mix of Lolium perenne L. (cvv. Speedster 35% and Greenway 35%)+Poa pratensis L. (cvv. SR2100 15% and Greenknight 15%). The goal of this trial was to evaluate turfgrass wear resistance and recovery devoid of climatic and soil effects and thus, sward growing, wear simulation and recovery were conducted in controlled environment. Furthermore, wear simulation was conducted with FIFA-approved, numerical control machinery (Lisport). Wear resistance and recovery data was plotted against results from laboratory investigations on key tissue constituents. Zoysia matrella proved to be the most wear resistant, but the slowest in recovery, whereas the bermudagrass hybrid showed the exact opposite behaviour. Lignin and carbohydrate concentrations proved to be the two factors most closely correlated with wear resistance and recovery respectively. These two classes of compounds were present in an equilibrium that was species specific, with a frequent mutual exclusion between lignin and starch concentrations that deserves further investigation at the intra-specific level.

Freeze tolerance of Zoysia matrella (L.) Merrill as affected by late-season nitrogen application, and changes in carbohydrates during cold acclimation

Abstract This study was conducted with the aim of (1) assessing the development of freeze tolerance (LT50) in “Zeon” zoysiagrass under the effect of two different levels (15 and 30 g m−2) of late-season nitrogen applications, and (2) evaluating the dynamics of nonstructural carbohydrates composition during cold acclimation and overwintering. A combined approach with natural acclimation in the field, followed by monthly controlled exposure to sub-freezing temperatures, was employed to evaluate LT50. Fall color of “Zeon” zoysiagrass was improved by nitrogen applications, the latter extending the green period by more than one month. In October, the higher nitrogen treatment caused significantly less cold hardiness (LT50=−10.0°C) compared with the other treatments, but was beneficial to freeze tolerance in March. The controls reached cold hardiness in November, 1 month earlier than the N treatments, in agreement with the color retention data. Controls also showed a more linear freeze tolerance during the experimental period. Significant variations in total soluble sugars occurred in zoysiagrass tissues during the acclimation and de-acclimation process. In particular, in leaves and, partially, in stems, total soluble sugars (TSS) levels were associated with LT50, while sprigs and roots did not show any significant association between LT50 and TSS. Alteration of metabolism was more evident in the 30-g N treatment, in particular as regards the storage sink.

GeoEye-1 satellite versus ground-based multispectral data for estimating nitrogen status of turfgrasses

Satellite remote sensing of leaf nitrogen (N) content is an interesting technique for agricultural crops for both economic and environmental reasons since it allows the monitoring of fertilization, and hence can potentially reduce the application of N according to real plant needs. The objective of this trial was to compare the N status in different turfgrasses using both remote multispectral data acquired by GeoEye-1 satellite and two ground-based instruments. The study focused on creating a N content gradient on three warm-season turfgrasses, (Cynodon dactylon × transvaalensis ‘Patriot’, Paspalum vaginatum ‘Salam’, Zoysia matrella ‘Zeon’), and two cool-season (Festuca arundinacea ‘Grande’, Lolium perenne ‘Regal 5’). The linear gradient of applied N ranged from 0 to 342 kg ha−1 for the warm-season and from 0 to 190 kg ha−1 for the cool-season turfgrasses. Proximity and remote-sensed reflectance measurements were acquired and used to determine the normalized difference vegetation index (NDVI). Our results proved that proximity-sensed NDVI is highly correlated with data acquired from satellite imagery. The correlation coefficients between data from the satellite and the other sensors ranged from 0.90 to 0.99 for the warm-season and from 0.83 to 0.97 for the cool-season species. ‘Patriot’ had a clippings N content ranging from 1.20% to 4.1%, thus emerging as the most reactive species to N fertilization. As such, the GeoEye-1 satellite can adequately assess the N status of different turfgrass species and its spatial variability within a field, depending on the N rates applied. In future, information obtained from satellites could allow precision fertilizer management on sports fields, golf courses, or other extended green areas.

Carbohydrate content, characterization and localization in bermudagrass stolons during establishment

Abstract Turfgrasses are continuously exposed to a wide range of detrimental stresses, such as mowing, traffic, low or high temperatures, salinity, drought, UV, hypoxia etc. Plant responses to stimuli involve nearly every aspect of plant physiology and metabolism. Carbohydrates, primary sucrose and starch, as principal metabolic products of photosynthesis, are stored in bermudagrass (Cynodon spp.) in stolons and rhizomes. Total nonstructural carbohydrates (TNC) levels have been used as indicators of health and physiological status in bermudagrass. TNC levels vary during the year and are weakly affected by N source. Establishment is a critical phase of bermudagrass management. The objectives of this research were to assess and characterize stolon carbohydrate content in nine bermudagrass cultivars during the full establishment phase in relation to establishment rates. Morphological and growth analyses showed distinct properties among the cultivars selected for this study. Significant differences in turf coverage among cultivars on each rating date were present starting from early growth stages. Carbohydrates localization was used to differentiate bermudagrass cultivars. The relationships between different carbohydrates and their role on growth were also explored. Gradients along the stolon from tip to older internodes were evident for starch. Our study provides further insight into bermudagrass physiology, with cultivar differences in carbohydrates production and localization providing valuable selection information for turfgrass breeders and practitioners, especially during the first year. Our data indicate that TNC levels were affected by cultivar and stolon maturity level. TNC content was found to correlate with establishment rate.

Effects of Application of Municipal Solid Waste Compost on Horticultural Species Yield

Composting as a means of waste recycling in order to produce organic fertilizer has long been practised by farmers. Agronomic interest in compost is due to its elevated nutrient and organic matter content. However compost could have an adverse impact on account of its salinity or the presence of pathogens or toxic metals. Numerous studies on this last aspect have shown that heavy metal content in compost treated plant does not differ significantly from the control (Massantini et al., 1988; Stilwell, 1993). The agronomic trials carried out on compost have shown conflicting results for marked variability in composition, type of species grown and pedoclimatic characteristics of the trial area (Del Zan, 1989; Edwards et al., 1993; Paris et al., 1986). The purpose of the present research was to study the direct and residual agronomic effects of compost from treated municipal solid waste on horticultural species.

Preliminary investigation on the potential use of two C4 turfgrass species to reduce nutrient release in a Mediterranean drained peatland

This study compared dry matter production, nutrient uptake and tissue nutrient concentration of two C4 turfgrass species (Cynodon dactylon × Cynodon transvaalensis Burtt Davy and Paspalum vaginatum Swartz) supplied with three different nutrient solutions in a sand and peat culture. The 8-week experiment was performed in mesocosms and simulated the conditions of an open-field phyto-treatment system located in a Mediterranean drained peatland (Tuscany, Italy). Peat was collected on the site, and one of the solutions mimicked drainage water flowing into it. Three hypotheses were tested: (i) the species chosen efficiently removed nutrients from both the solution and the substrate; (ii) peat contributed to the nutrient load; and (iii) the species chosen were suitable in the open-field system. Both species adapted well to the experimental conditions and demonstrated considerable ability to remove nutrients. P. vaginatum took up nitrogen more efficiently, mainly in conditions of high nutrient availability. We observed supplementary nutrient uptake by plants in the peat treatment. Performances of the two C4 turfgrasses extrapolated to the field scale seemed effective from a phyto-treatment perspective.

Autonomous Mower Saves Energy and Improves Quality of Tall Fescue Lawn

Battery-powered autonomous mowers are designed to reduce the need of labor for lawn mowing compared with traditional endothermic engine mowers and at the same time to abate local emissions and noise. The aim of this research was to compare autonomousmower with traditional rotary mower on a tall fescue (Festuca arundinacea) lawn under different nitrogen (N) rates. A two-way factor experimental design with three replications was adopted. In the study, four N rates (0, 50, 100, and 150 kg ha) and two mowing systems (autonomous mower vs. gasolinepowered walk-behind rotary mower equipped for mulching) were used. As expected, N fertilization increased turf quality. At the end of the trial, the autonomous mower increased turf density (3.2 shoots/cm) compared with the rotary mower (2.1 shoots/cm) and decreased average leaf width (2.1 mm) compared with the rotary mower (2.7 mm). Increased density and decreased leaf width with autonomous mowing yielded higher quality turf (7.3) compared with the rotary mower (6.4) and a lower weed incidence (6% and 9% cover for autonomous mower and rotary mower, respectively). Disease incidence and mowing quality were unaffected by the mowing system. The autonomous mower working timewas set to 10 hours per day ( 7.8 hours formowing and 2.2 hours for recharging) for a surface of 1296 m. The traditional rotary mower working time for the same surface was 1.02 hours per week. The estimated primary energy consumption for autonomous mower was about 4.80 kWh/week compared with 12.60 kWh/week for gasoline-powered rotary mowing. Based on turf quality aspects and energy consumption, the use of autonomous mowers could be a promising alternative to traditional mowers.

Turfgrass spectral reflectance: simulating satellite monitoring of spectral signatures of main C3 and C4 species

In recent years, within the European Union several legislative, monitoring and coordinating actions have been undertaken to encourage sustainable use of resources, reduction in the use of chemicals and improvement of the urban environment. In this respect, two concepts that are strictly related to most of the aspects above are: “precision agriculture” and “precision conservation” and more specifically “precision turfgrass management.” Optical sensing has become a crucial part of precision turfgrass management and spectral reflectance in particular has been an active area of research for many years. However, while turfgrass status evaluation by proximity-sensed spectral reflectance appears to be an established and reliable practice, much more could be achieved in terms of monitoring of large turfgrass areas through remote sensing, and in particular through satellite imagery. This paper reports the results of a trial attempting to evaluate the spectral signatures of several turfgrass species and cultivars, for future use in turfgrass satellite monitoring. Our experimental study focused on 20 turfgrass species/varieties including perennial ryegrasses, tall fescues, kentucky bluegrasses, bermudagrass ecotypes, seeded commercial bermudagrasses, vegetatively propagated bermudagrasses, Zoysia japonica and non-japonica zoysiagrasses. Various biological and agronomical parameters were studied and turfgrass spectral reflectance for all entries was gathered. Vegetation indices were calculated by simulating the available wavelengths deriving from World View 2 satellite imagery. Results showed that within the same species selected vegetation indices are often able to discriminate between different varieties that have been established and maintained with identical agronomical practices.