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Dive into the research topics where Marcus Vinícius Lia Fook is active.

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Featured researches published by Marcus Vinícius Lia Fook.


Archive | 2012

Application of Infrared Spectroscopy to Analysis of Chitosan/Clay Nanocomposites

Suédina M.L. Silva; Carla R.C. Braga; Marcus Vinícius Lia Fook; Claudia M. O. Raposo; Laura H. Carvalho; Eduardo L. Canedo

In recent years, polymer/clay nanocomposites have attracted considerable interest because they combine the structure and physical and chemical properties of inorganic and organic materials. Most work with polymer/clay nanocomposites has concentrated on synthetic polymers, including thermosets such as epoxy polymers, and thermoplastics, such as polyethylene, polypropylene, nylon and poly(ethylene terephthalate) (Pandey & Mishra, 2011). Comparatively little attention has been paid to natural polymer/clay nanocomposites. However, the opportunity to combine at nanometric level clays and natural polymers (biopolymers), such as chitosan, appears as an attractive way to modify some of the properties of this polysaccharide including its mechanical and thermal behavior, solubility and swelling properties, antimicrobial activity, bioadhesion, etc. (Han et al., 2010). Chitosan/clay nanocomposites are economically interesting because they are easy to prepare and involve inexpensive chemical reagents. Chitosan, obtained from chitin, is a relatively inexpensive material because chitin is the second most abundant polymer in nature, next to cellulose (Chang & Juang, 2004). In the same way, clays are abundant and low-cost natural materials. Although chitosan/clay nanocomposites are very attractive, they were not extensively investigated, with relatively small number of scientific publications. In addition, the successful preparation of the nanocomposites still encounters problems, mainly related to the proper dispersion of nano-fillers within the polymer matrix. In this chapter, in addition to discussing the synthesis and characterisation by infrared spectroscopy of chitosan/clay nanocomposites, data of x-ray diffraction and mechanical properties are also considered.


PLOS ONE | 2015

Protection against T1DM-Induced Bone Loss by Zinc Supplementation: Biomechanical, Histomorphometric, and Molecular Analyses in STZ-Induced Diabetic Rats

Raul Hernandes Bortolin; Bento João Abreu; Karla Simone Costa de Souza; João Felipe Bezerra; Melina Bezerra Loureiro; Flávio Santos da Silva; Dáfiny Emanuele da Silva Marques; Angélica Amanda de Sousa Batista; Gisele Oliveira; André Ducati Luchessi; Valéria Morgiana Gualberto Duarte Moreira Lima; Carlos Eduardo Saraiva Miranda; Marcus Vinícius Lia Fook; Maria das Graças Almeida; Luciana Augusto de Rezende; Adriana Augusto de Rezende

Several studies have established an association between diabetes and alterations in bone metabolism; however, the underlying mechanism is not well established. Although zinc is recognized as a potential preventive agent against diabetes-induced bone loss, there is no evidence demonstrating its effect in chronic diabetic conditions. This study evaluated the effects of zinc supplementation in a chronic (90 days) type 1 diabetes-induced bone-loss model. Male Wistar rats were distributed in three groups: control, type 1 diabetes mellitus (T1DM), and T1DM plus zinc supplementation (T1DMS). Serum biochemical analysis; tibia histomorphometric, biomechanical, and collagen-content analyses; and femur mRNA expression were evaluated. Relative to T1DM, the zinc-supplemented group showed increased histomorphometric parameters such as TbWi and BAr and decreased TbSp, increased biomechanical parameters (maximum load, stiffness, ultimate strain, and Young’s modulus), and increased type I collagen content. Interestingly, similar values for these parameters were observed between the T1DMS and control groups. These results demonstrate the protective effect of zinc on the maintenance of bone strength and flexibility. In addition, downregulation of OPG, COL1A, and MMP-9 genes was observed in T1DMS, and the anabolic effects of zinc were evidenced by increased OC expression and serum ALP activity, both related to osteoblastogenesis, demonstrating a positive effect on bone formation. In contrast, T1DM showed excessive bone loss, observed through reduced histomorphometric and biomechanical parameters, characterizing diabetes-associated bone loss. The bone loss was also observed through upregulation of OPG, COL1A, and MMP-9 genes. In conclusion, zinc showed a positive effect on the maintenance of bone architecture and biomechanical parameters. Indeed, OC upregulation and control of expression of OPG, COL1A, and MMP-9 mRNAs, even in chronic hyperglycemia, support an anabolic and protective effect of zinc under chronic diabetic conditions. Furthermore, these results indicate that zinc supplementation could act as a complementary therapy in chronic T1DM.


Key Engineering Materials | 2008

Porous Hydroxyapatite Scaffolds by Polymer Sponge Method

A.C.B.M. Fook; Anahi Herrera Aparecida; Thiago Bizerra Fideles; R.C. Costa; Marcus Vinícius Lia Fook

This study aimed to develop porous hydroxyapatite scaffold for bone regeneration using the replica of the polymeric sponge technique. Polyurethane sponges were used with varying densities to obtain the scaffolds. The results indicate the porous HA scaffolds developed in this study as potential materials for application as bone substitutes to have high porosity (> 70%), chemical composition, interconnectivity and pore sizes appropriate to the bone regeneration.


Basic & Clinical Pharmacology & Toxicology | 2017

Anabolic Effect of Insulin Therapy on the Bone: Osteoprotegerin and Osteocalcin Up-Regulation in Streptozotocin-Induced Diabetic Rats.

Raul Hernandes Bortolin; Francisco Paulo Freire Neto; Carlos Alberto Arcaro; João Felipe Bezerra; Flávio Santos da Silva; Karla Simone da Costa Souza; Valéria Morgiana Gualberto Duarte Moreira Lima; André Ducati Luchessi; Francisco Pignataro Lima; Marcus Vinícius Lia Fook; Bartolomeu Jorge da Silva; Maria das Graças Almeida; Bento João Abreu; Luciana Augusto de Rezende; Adriana Augusto de Rezende

Type 1 diabetes mellitus (T1DM) is associated with several skeletal alterations, particularly in conditions of poor glycaemic control. Insulin therapy is the major conservative treatment for T1DM; however, the effects of this hormone on bone markers of T1DM rats are limited, and the regulatory mechanisms remain elusive. Therefore, the evaluation of molecular and non‐molecular parameters in a chronic animal model of T1DM‐induced bone loss, treated with and without insulin, may help in elucidating the insulin mechanisms. Male Wistar rats were assigned into three groups: control, T1DM (T1DM rats induced with streptozotocin [STZ] at 40 mg/kg intravenously) and T1DM plus insulin therapy (T1DMI). After 8 weeks, we evaluated the serum biochemical, tibia histomorphometric and biomechanical parameters, as well as the gene expression of the receptor activator of nuclear factor kappa‐B ligand (RANKL), osteoprotegerin (OPG) and osteocalcin (OC) of femur mRNA. Compared with T1DM, the T1DMI group showed less bone loss, which was revealed by the increased trabecular width (TbWi, p < 0.001) and trabecular bone area (BAr, p < 0.01), reduced trabecular separation (TbSp, p < 0.01) and increased Youngs modulus (p < 0.05). Moreover, molecular analyses indicated that the expression of OPG and OC was up‐regulated (p < 0.001 and p < 0.05, respectively). In summary, the up‐regulation of OPG and OC in the T1DMI group supports an anabolic effect of insulin, which was demonstrated by the maintenance of bone architecture and flexibility. These results suggest that insulin therapy may prevent T1DM‐induced bone loss via the effects on the bone formation.


Ciencia Rural | 2013

Hidroxiapatita e quitosana isoladas e associadas à medula óssea no reparo do tecido ósseo em coelhos. Estudo histológico e morfométrico

Adílio Santos de Azevedo; Marcelo Jorge Cavalcanti de Sá; Marcus Vinícius Lia Fook; Pedro Isidro da Nóbrega Neto; Otávio Brilhante de Sousa; Sérgio Santos de Azevedo

The aim of this research was to evaluate the process of bone regeneration in rabbits by the use of hydroxyapatite and chitosan only and associated with bone marrow. Twenty male and female rabbits, New Zealand breed and weighing in average 3.0±0.4kg were used. Animals were randomly divided according with two experimental moments, being 10 animals euthanized 30 days after surgery, and 10 euthanized 60 days after surgery. Four implants that constituted the groups were used. Materials were introduced into the proximal (MP) and distal metaphysis (MD) of each femur, so that chitosan was introduced into the MP and hydroxyapatite into the MD of the left limb, and in the right limb bone marrow was added beyond the implants. At the comparative descriptive histological evaluation, it was found that the QUI and QUIMO groups in both periods stimulated more pronounced reactions and accelerated bone repair. In morphometric analysis, QUI and QUIMO groups differed statistically from the other, has been shown greater bone formation (P<0.05).


Materia-rio De Janeiro | 2010

Desenvolvimento de biocerâmicas porosas de hidroxiapatita para utilização como scaffolds para regeneração óssea

A.C.B.M. Fook; A.H. Aparecida; Marcus Vinícius Lia Fook

Porous bioceramics have important biomedical applications such as bone defect fillers and tissue engineering scaffolds. The hydroxyapatite (HA, Ca10 (Po4)6(OH)2) shows resemblance to the mineral phase of bone and teeth, it is biocompatible and osteoconductive, and has excellent chemical and biological affinity to bone tissue. The aim of this work is to develop HA porous bioceramics for bone regeneration using the replica of the polymeric sponge technique. The HA slurry bioceramic was obtained by wet method using by the calcium hydroxide [Ca (OH) 2] and phosphoric acid (H3PO4) and impregnated polyurethane sponges with different densities. Heat treatment at 600 ° C for 1h was accomplished to eliminate the sponge followed by sintering at 1100 ° C for 2 hours. The scaffolds showed HA as major phase, high porosity (> 70%) and pores with sizes ranging in the order of macro (> 100μm) and microporosity (1-20μm), and these factors are suitable for application as scaffolds for bone regeneration.


Materials Science Forum | 2014

Evaluation of the Cytotoxic Potential Chitosan/Hydroxyapatite Biocomposites

Rita de Cássia A. Leal; I.V.S.R. Nascimento; Marcus Vinícius Lia Fook; Roberta Costa Meira

The incorporation of calcium phosphate in a polymer matrix for composites production combines the flexibility of the polymers with the resistance, hardness and bioactivity of the inorganic phase. This study aimed to obtain rigid biocomposites using hydroxyapatite as mineral phase and chitosan as a polymer binder for the application as a biomaterial. To obtain the biocomposites, hydroxyapatite was added under constant stirring to a solution of chitosan in order to satisfy a 70:30 (HA/CS) ratio. XRD results showed no significant change in the profile of hydroxyapatite with the incorporation of chitosan. The analysis of scanning electron microscopy (SEM) of the biocomposite HA/CS, indicated that there was an excellent dispersion of HA particles in the polymeric matrix. Infrared Spectroscopy with Fourier Transform (FTIR) analysis proved an existing interaction between chitosan and hydroxyapatite. The biocompatibility of biocomposites HA/CS was confirmed by the MTT assay. It is considered that the present biocomposites are a promising biomaterial for trabecular bone grafting


Materials Science Forum | 2014

Biodegradable Chitosan Scaffolds: Effect of Genipin Crosslinking

Greyce Yane Honorato Sampaio; A.C.B.M. Fook; Thiago Bizerra Fideles; M.E.R.R.M. Cavalcanti; Marcus Vinícius Lia Fook

Currently researchers has pointed chitosan as one of the viable alternatives for application as scaffolds in tissue regeneration, mainly due to its availability, biocompatibility, biodegradability and ability to chemical modifications, among them, the crosslinking. With the growing number of investigations of crosslinking agents from natural sources and its applicability, this work focuses on the development and microstructural characterization of chitosan scaffolds and chitosan crosslinked with genipina using the technique of freeze drying. Solutions were prepared with chitosan concentration of 2% (w / t), and genipin 0.15% (w / t) and 0.3% (w / t). These were frozen at-20 ° C and ≈ 196 ° C, and lyophilized. The effect of different concentrations of genipin and freezing rates of the solutions in the porous architecture of the scaffolds were evaluated by Scanning Electron Microscopy (SEM), Optical Digital Microscopy 2D and 3D (OM) and testing the Degree of Swelling (DS). The SEM analysis revealed the formation of a three dimensional structure with pores and / or channels interconnected, influenced by the freezing rate and addition of crosslinking agent, these variables also influence the absorption capacity of the scaffolds. It was observed by OM, the effectiveness of the crosslinking with genipin, through points of fluorescence presented.


Arquivo Brasileiro De Medicina Veterinaria E Zootecnia | 2014

Utilização do filme de quitosana na reparação de tendão em coelhos

Clarice Carvalho Santana; P. I. Nóbrega Neto; Marcelo Jorge Cavalcanti de Sá; L. M. Oliveira; Marcus Vinícius Lia Fook; Adílio Santos de Azevedo; Otávio Brilhante de Sousa

This work aimed to evaluate the process of tendon healing in rabbits, using the treatment group in chitosan film through a clinical, surgical and histological analysis. In the experiment, 12 adult rabbits were used, divided into control group (CG) and treatment group (TG), which were held in the partial section of the gastrocnemius tendon of both limbs. Clinical assessment was based on the presence of inflammation, infection, pain and suture dehiscence. For histological evaluation a comparative study of the healing process through the cell type, amount of connective tissue and collagen fiber organization was performed between groups and times. In surgical wounds no secretion, pain or dehiscence were observed. In the comparative histology between groups, the CG showed better healing compared to TG, at 60 days. On day 90 the TG already outlines tendon recovery with reorganization of cellularity and collagen fibers in the dense connective tissue modeled. It was concluded that chitosan stimulates faster cell growth, but it is haphazardly, and that complete healing occurs after 90 days of their implantation in tissue.


Materials Science Forum | 2014

Sterilization of Chitosan Membranes for Use as Biomaterial

I.V.S.R. Nascimento; Rita de Cássia A. Leal; Marcus Vinícius Lia Fook; Roberta Costa Meira

Sterilization is a procedure which promotes complete destruction or elimination of all forms of microorganisms. It is a fundamental stage in the processing of biomaterials, and functionality of any sterilization system must be determined by its effectiveness in exterminating microorganisms without harming or adversely affect the properties of medical devices. Some sterilization techniques can react with functional groups of the polymer leading to changes in the properties, affecting the biomaterial. This study aimed to develop chitosan membranes sterilize them by following methods: an autoclave, ethylene oxide, glutaraldehyde and ultraviolet radiation and characterized by FTIR, XRD and SEM. Based on the results of this study it can be concluded that the method of sterilization indicated for the membranes of chitosan is ethylene oxide because the other altered physicochemical properties thereof.

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Thiago Bizerra Fideles

Federal University of Campina Grande

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Rossemberg Cardoso Barbosa

Federal University of Campina Grande

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A.C.B.M. Fook

Federal University of Campina Grande

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I.V.S.R. Nascimento

Federal University of Campina Grande

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Daniel B. Lima

Federal University of Campina Grande

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Silvia M. P. Borges

Federal University of Campina Grande

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Adílio Santos de Azevedo

Federal University of Campina Grande

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Carmem Dolores de Sá Catão

Federal University of Campina Grande

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Elaine Patrícia Araújo

Federal University of Campina Grande

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