Marek Stepniewski

Prolonged Quercetin Administration Diminishes the Etoposide-Induced DNA Damage in Bone Marrow Cells of Rats

The DNA damage in bone marrow cells induced by etoposide (E) injected intraperitoneally to rats (100 mg/kg b.w.) decreased to the control level when quercetin (Q) was administered subcutaneously for 10 consecutive days (40 mg/kg b.w.per day) before E was injected. The antioxidant power (FRAP assay) increased significantly after Q or E compared with control rats but did not change when Q preceded the E injection. The superoxide dismutase activity significantly increased in Q+E-treated rats compared with quercetin given alone. The study provides evidence that Q protects bone marrow cells against long-lived E-induced DNA damage and alters the redox balance in lung tissue.

Pattern recognition methods in evaluation of the structure of the laboratory data biominerals, antioxidant enzymes, selected biochemical parameters, and pulmonary function of welders

A wide set of biochemical and spirometrical parameters was applied to a group of 41 welders. The obtained parameters were analyzed by the pattern recognition method: principal-components analysis (PCA) and mutual contribution analysis (MCA). The aim of the investigation was to find out which of 23 parameters had an influence on the pulmonological indices of COPD. It was found that PCA gave a substantial reduction of dimensionality. The most relevant parameters were Fe, Zn, Cu (all elements in blood, not in hair), FEV1, MEF50, bilirubin, TAS, and SOD. Only the relationships of MEF50, Cu, and TAS were statistically valid.

Micronutrients and macronutrients and parameters of antioxidative ability in saliva of women: inhabitants of Krakow (Poland) in the course of uncomplicated singleton pregnancy.

There were two aims of the present study: (1) to evaluate changes of superoxide dismutase (SOD) activity and total antixoidative status measured as the ferric reducing ability of plasma (FRAP) concentration in saliva of pregnant women during the first, second, and third trimesters of singleton uncomplicated pregnancy and (2) to assess possible relations among SOD, FRAP, and intake of macronutrients and micronutritients in daily nutritional rations (DNRs) during pregnancy. Forty pregnant women aged 27.1±5.4 yr (examined group) and 40 healthy women (the control group) were recruited for this study. The relationship between FRAP and SOD in saliva and the intake of macronutrients (proteins, carbohydrates, total fat, saturated fatty acid, monounsaturated fatty acids, polyunsaturated fatty acids), minerals (Na, K, Ca, P, Mg, Fe, Zn, Cu, Mn), and vitamins (A, C, E, B1, B2, B6, PP) in DNR was evaluated by clustering analysis with Wards grouping method. During pregnancy, FRAP and SOD values were lower than in the controls, but only for FRAP were the differences statistically significant (p<0.001). For the whole pregnancy period, cluster analysis identified two major clusters for which the differentiating variables were SOD and retinoids intake, but different patterns for each trimester of pregnancy were revealed. The following were concluded: (1) FRAP values were the lowest in the second trimester. It suggests that in this trimester of uncomplicated pregnancy, women might be not fully adapted to increased demands for antioxidative mechanisms. (2) Cluster analysis showed that there were no statistical relationships between the intake of micronutrients and macronutrients in DNRs and the SOD or FRAP level in the saliva of pregnant women.

C-reactive protein as a marker of heart failure.

Committees • European Society of Cardiology: Working Group on Valvular Heart Disease Chairperson: Christa Gohlke-Bärwolf Vice-chairperson: Roger J.C. Hall • Presidents of the Organizing Committee and Guest Editors Wies¥awa Tracz – Director of the Institute of Cardiology, Jagiellonian University, Krakow Piotr Podolec – President of the Polish Cardiac Society, Krakow Division • Secretaries: Piotr Musia¥ek, Wojciech P¥azak, Monika Pieculewicz