María Alejandra Martínez

Distribution of flavonoid aglycones in Ilex species (Aquifoliaceae)

Abstract Leaf flavonoids of 59 Ilex species were analysed. Three flavonols: kaempferol, quercetin and isorhamnetin, and two flavones: apigenin and luteolin, were identified. This is the first report of isorhamnetin and flavones in the genus Ilex. The possible phylogenetic significance of the compounds found as well as the taxonomic value of the flavones in certain species are discussed.

Cellulose degrading bacteria isolated from industrial samples and the gut of native insects from Northwest of Argentina

The raw materials used to produce bioethanol mostly are food crops, which has led to conflicts on food security. It is, therefore, recommended the gradual replacement for second generation substrates such as lignocellulosic materials. Herein, cellulolytic bacteria were isolated from the gut content of native larvae from Lepidoptera, Coleoptera, and adults of Isoptera. Few environmental samples from the pulp and paper feedstock were also assessed. A total of 233 isolates were obtained using enrichment cultures and classic criteria. Interestingly, several halo‐forming colonies were found to be bacterial consortia that presented difficulties to take apart the microbial members. Those pure isolates which hydrolyzed cellulose in larger extend (45 strains) were selected and identified by means of 16S rRNA sequence analysis. Firmicutes was the prevalent phylum (62.2%) being Bacillus spp. the most frequent genus, while Paenibacillus, Brevibacillus, Cohnella, and Staphylococcus species were less frequent. The phylum Actinobacteria (6.7%) was represented by isolates related to Agromyces spp. and Microbacterium spp. Regarding Gram‐negative bacteria (31.1%), the more depicted genus was Pseudomonas spp., and members of Achromobacter spp., Enterobacter spp., and Bacteroidetes phylum were also selected. These native bacterial strains are expected to enlarge the cellulolytic toolbox for efficient biomass deconstruction.

Flavonoid aglycones from Argentinian Capparis species (Capparaceae)

Abstract Seven Capparis species growing in Argentina were investigated for their leaf flavonoid aglycone pattern. Six flavonol aglycones were isolated and identified as kaempferol, quercetin, isorhamnetin, and their 7-O-methyl derivatives: rhamnocitrin, rhamnetin, and rhamnazin, respectively. This is the first report of methylated flavonoids in the genus Capparis .

Two amplicon sequencing strategies revealed different facets of the prokaryotic community associated with the anaerobic treatment of vinasses from ethanol distilleries

The prokaryotic consortium from a pilot-scale UASB reactor fed with vinasses from ethanol distilleries was evaluated by means of amplicon sequencing of the 16S rRNA gene. Two different sets of primers targeted to overlapping regions of the V4-16S region were used to gain a broad picture of such community and to perform a comparative analysis. From the two datasets obtained, prevalent phyla were Firmicutes, Verrucomicrobia and Thermotogae. Interestingly, one set of primers captured variability in both the bacterial and archaeal portions of the community, whilst the other one revealed a more diverse community structure, but only in the Bacteria domain. Although a certain level of agreement between the two strategies was observed, sharp differences indicate that different facets of the community were disclosed by each approach.

Endoglucanase and xylanase production by Bacillus sp. AR03 in co-culture

ABSTRACT The behavior of three isolates retrieved from different cellulolytic consortia, Bacillus sp. AR03, Paenibacillus sp. AR247 and Achromobacter sp. AR476-2, were examined individually and as co-cultures in order to evaluate their ability to produce extracellular cellulases and xylanases. Utilizing a peptone-based medium supplemented with carboxymethyl cellulose (CMC), an increase estimation of 1.30 and 1.50 times was obtained by the co-culture containing the strains AR03 and AR247, with respect to enzyme titles registered by their individual cultivation. On the contrary, the extracellular enzymatic production decreased during the co-cultivation of strain AR03 with the non-cellulolytic Achromobacter sp. AR476-2. The synergistic behavior observed through the combined cultivation of the strains AR03 and AR247 might be a consequence of the consumption by Paenibacillus sp. AR247 of the products of the CMC hydrolysis (i.e., cellobiose and/or cello-oligosaccharides), which were mostly generated by the cellulase producer Bacillus sp. AR03. The effect observed could be driven by the requirement to fulfill the nutritional supply from both strains on the substrate evaluated. These results would contribute to a better description of the degradation of the cellulose fraction of the plant cell walls in nature, expected to an efficient utilization of renewable sources.

Raw sugarcane bagasse as carbon source for xylanase production by Paenibacillus species: a potential degrader of agricultural wastes

Paenibacillus species isolated from a variety of natural sources have shown to be important glycoside hydrolases producers. These enzymes play a key role in bio-refining applications, as they are central biocatalysts for the processing of different types of polymers from vegetal biomass. Xylanase production by three native isolates belonging to the genus Paenibacillus was approached by utilizing mineral-based medium and agricultural by-products as a convenient source to produce biocatalysts suitable for their degradation. While varieties of alkali pretreated sugarcane bagasse were useful substrates for the strains from Paenibacillus genus evaluated, raw sugarcane bagasse was the most effective substrate for endoxylanase production by Paenibacillus sp. AR247. This strain was then selected to further improvement of its enzyme production by means of a two-step statistical approach. It was determined that the carbon source, provided as an inexpensive agro-waste, as well as phosphate and magnesium were the culture media components that most influenced the enzyme production, which was improved three times compared to the screening results.

Designing cross-linked xylanase aggregates for bioconversion of agroindustrial waste biomass towards potential production of nutraceuticals

Immobilized biocatalysts design has the potential to efficiently produce valuable bioproducts from lignocellulosic biomass. Among them, the carrier-free immobilization through the cross-linked enzyme aggregates technology is a simple and low-cost alternative. A two steps statistical approach was utilized to evaluate the synthesis of a cross-linked enzyme aggregate from a xylanolytic preparation, which was produced by Cohnella sp. AR92 grown in a peptone-based culture medium. The resulting immobilized biocatalyst, Xyl-CLEA, was significate more stable (25 to 45%) towards temperatures up to 50°C with respect to the free enzyme, and retained over 50% of its initial activity after 5 consecutive cycles of reuse. By means of infrared spectroscopy and electron microscopy, the Xyl-CLEA showed architectural features described as signature of type I and type II of protein aggregates. These, were the result of the simultaneous aggregation of a multiplicity of proteins from the crude enzymatic extract. The enzymatic activity was assessed using alkali pretreated sugar cane bagasse as substrate. Whereas the free enzymatic preparation released xylose as the main product, the immobilized xylanase produced xylooligosaccharides, thus showing that the immobilization procedure modified the potential application of the extracellular xylanase from Conhella sp. AR92.

Agrowastes as Feedstock for the Production of Endo-β-Xylanase from Cohnella sp. Strain AR92

Members of Cohnella sp. isolated from a variety of environments have been shown to be glycoside hydrolase producers. Nevertheless, most evaluations of members of this genus are limited to their taxonomic description. The strain AR92, previously identified as belonging to the genus Cohnella, formed a well-supported cluster with C. thailandensis and C. formosensis (>80% bootstrap confidence). Its growth and xylanase production were approached by using a mineral-based medium containing alkali-pretreated sugarcane bagasse as the main carbon source, which was assayed as a convenient source to produce biocatalysts potentially fitting its degradation. By means of a two-step statistical approach, the production of endoxylanase was moderately improved (20%). However, a far more significant improvement was observed (145%), by increasing the inoculum size and lowering the fermentation temperature to 25°C, which is below the optimal growth temperature of the strain AR92 (37°C). The xylanolytic preparation produced by Cohnella sp. AR92 contained mild temperature-active endoxylanase (identified as redundant GH10 family) for the main activity which resulted in xylobiose and xylo-oligosaccharides as the main products from birchwood xylan.

Microbial Consortia, a Viable Alternative for Cleanup of Contaminated Soils

The growth population and anthropogenic activity are constantly threatening the environment caused by the accumulation of different kinds of pollutants in the biosphere, especially in soils and sediments. Co-contaminated of environment with toxic organic and inorganic substance is often actually. For the remediation of soils contaminated with mainly petroleum, pesticide and heavy metals, several physical or chemical techniques have been developed inadequately. Inside the technologies “eco-friendly remediation,” the bioremediation have emerged as an option using natural biological activity. Bioremediation are methods where microorganisms degrade one or various pollutants to nontoxic compounds, so working individually or coordinately inside a microbial consortium. A microbial consortium is the natural association of two or more microbial populations of different species, which act together in a complex system. The success of a bioremediation process with pure cultures is very low and restricted. Therefore, use of a microbial consortium appears to be more feasible and reliable.

Fungal Nanotechnology: A New Approach Toward Efficient Biotechnology Application

Nanotechnology is a wide developing area of the biotechnology since the important applications of nanoparticles (NPs) in different technologies. The NPs produced by green technologies have many advantages such as greater surface area and high catalytic activity, in addition to providing a suitable contact between the metal salt and enzyme. Fungi secrete proteins, enzymes, and reducing agents which can be used for the synthesis of metal NPs from metal salts.