María Dolores Fariñas

Shear waves in vegetal tissues at ultrasonic frequencies

Shear waves are investigated in leaves of two plant species using air-coupled ultrasound. Magnitude and phase spectra of the transmission coefficient around the first two orders of the thickness resonances (normal and oblique incidence) have been measured. A bilayer acoustic model for plant leaves (comprising the palisade parenchyma and the spongy mesophyll) is proposed to extract, from measured spectra, properties of these tissues like: velocity and attenuation of longitudinal and shear waves and hence Young modulus, rigidity modulus, and Poissons ratio. Elastic moduli values are typical of cellular solids and both, shear and longitudinal waves exhibit classical viscoelastic losses. Influence of leaf water content is also analyzed.

The Application of Leaf Ultrasonic Resonance to Vitis vinifera L. Suggests the Existence of a Diurnal Osmotic Adjustment Subjected to Photosynthesis

The main objective of this study was to apply the air-coupled broad-band ultrasonic spectroscopy in attached transpiring leaves of Vitis vinifera L. to monitor changes in leaf water potential (Ψ) through the measurements of the standardized value of the resonant frequency associated with the maximum transmitance (f/fo). With this purpose, the response of grapevine to a drought stress period was investigated in terms of leaf water status, ultrasounds, gas exchange and sugar accumulation. Two strong correlations were obtained between f/fo and Ψ measured at predawn (pd) and at midday (md) with different slopes. This fact implied the existence of two values of Ψ for a given value of f/fo, which was taken as a sign that the ultrasonic technique was not directly related to the overall Ψ, but only to one of its components: the turgor pressure (P). The difference in Ψ at constant f/fo (δ) was found to be dependent on net CO2 assimilation (A) and might be used as a rough estimator of photosynthetic activity. It was then, the other main component of Ψ, osmotic potential (π), the one that may have lowered the values of md Ψ with respect to pd Ψ by the accumulation of sugars associated to net CO2 assimilation. This phenomenon suggests the existence of a diurnal osmotic adjustment in this species associated to sugars production in well-watered plants.

Ultrasonic spectroscopy allows a rapid determination of the relative water content at the turgor loss point: a comparison with pressure–volume curves in 13 woody species

The turgor loss point (TLP), which is considered a threshold for many physiological processes, may be useful in plant-breeding programs or for the selection of reforestation species. Obtaining TLP through the standard pressure-volume (p-v) curve method in a large set of species is highly time-consuming and somewhat subjective. To solve this problem, we present an objective and a less time-consuming technique based on the leaf resonance able to calculate the relative water content (RWC) at TLP (RWCTLP). This method uses air-coupled broadband ultrasonic spectroscopy to obtain the sigmoidal relation between RWC and the standardized resonant frequency (f/fo). For the 13 species measured, the inflexion point of the RWC-f/fo relationship ( ) was not statistically different from the value of RWC at the TLP obtained with the p-v curves (RWCTLP p-v).

MONITORING PLANT RESPONSE TO ENVIRONMENTAL STIMULI BY ULTRASONIC SENSING OF THE LEAVES

Described here is the application of a technique based on the excitation, sensing and spectral analysis of thickness resonances of plant leaves using air-coupled and wide-band ultrasound pulses (150-900 kHz) to monitor variations in leaf properties caused by plant responses to different environmental stimuli, such as a sudden variation in light intensity (from 2000 to 150 μmol m(-2) s(-1)), sudden watering after a drought period, and along the diurnal cycle (3-5 days, with continuous variation in light intensity from 150 to 2000 μmol m(-2) s(-1) and change in temperature of about 5°C). Four different widely available species, both monocots and dicots and evergreen and deciduous, with different leaf features (shape, size, thickness, flatness, vascular structure), were selected to test the technique. After a sudden decrease in light intensity, and depending on the species, there was a relative increase in the thickness resonant frequency from 8% to 12% over a 25- to 50-min period. After sudden watering, the relative increase in the resonant frequency varied from 5% to 30% and the period from 10 to 400 min. Finally, along the diurnal cycle, the measured relative variation is between 4% and 10%. The technique revealed differences in both the amplitude of the frequency oscillations and the kinetics of the leaf response for different species and also within the same species, but for specimens grown under different conditions that present different cell structures at the tissue level. The technique can be equally applied to the leaves of any species that present thickness resonances.

Non-contact ultrasonic inspection of CFRP prepregs for aeronautical applications during lay-up fabrication

The possibility to inspect laminates of prepreg carbon fiber reinforced polymer (CFRP) laminates during lay-up fabrication is studied. First ultrasonic properties of the uncured material are determined, this information is used to design an inspection system that is tested during the fabrication of laminates with up to 30 layers, following different compaction schemes and including some Teflon insertions to simulate the presence of delaminations. The paper shows that for the chosen selection of parameters (transducers sensitivity, centre frequency and mold configuration), the inspection is possible, opening a new field of application of air-coupled ultrasonic techniques.

Ultrasonic Sensing of Plant Water Needs for Agriculture

Fresh water is a key natural resource for food production, sanitation and industrial uses and has a high environmental value. The largest water use worldwide (~70%) corresponds to irrigation in agriculture, where use of water is becoming essential to maintain productivity. Efficient irrigation control largely depends on having access to reliable information about the actual plant water needs. Therefore, fast, portable and non-invasive sensing techniques able to measure water requirements directly on the plant are essential to face the huge challenge posed by the extensive water use in agriculture, the increasing water shortage and the impact of climate change. Non-contact resonant ultrasonic spectroscopy (NC-RUS) in the frequency range 0.1–1.2 MHz has revealed as an efficient and powerful non-destructive, non-invasive and in vivo sensing technique for leaves of different plant species. In particular, NC-RUS allows determining surface mass, thickness and elastic modulus of the leaves. Hence, valuable information can be obtained about water content and turgor pressure. This work analyzes and reviews the main requirements for sensors, electronics, signal processing and data analysis in order to develop a fast, portable, robust and non-invasive NC-RUS system to monitor variations in leaves water content or turgor pressure. A sensing prototype is proposed, described and, as application example, used to study two different species: Vitis vinifera and Coffea arabica, whose leaves present thickness resonances in two different frequency bands (400–900 kHz and 200–400 kHz, respectively), These species are representative of two different climates and are related to two high-added value agricultural products where efficient irrigation management can be critical. Moreover, the technique can also be applied to other species and similar results can be obtained.

Visualization of lamb wave propagation in uncured CFRP and curved surfaces using air-coupled ultrasound

Propagation of Lamb waves, generated and detected using air-coupled piezoelectric transducers (0.1-1.0 MHz), is visualized. Hence phase and group velocities are obtained. The technique is first tested on plates (aluminum and carbon fiber reinforced polymers-CFRP-plates). Then it has been applied to un-cured CFRPs plates and curved surfaces: steel pipes and vessels and to the curved section of CFRP beams. Two different experimental set-ups are proposed: 1) use of monolithic transducers and mechanical scans along the direction of propagation, 2) use of a phased array linear transducer and an electronic scan along the direction of propagation.

Assessment of the ultrasonic properties of additive manufactured materials for passive components of piezoelectric transducers

The potential of additive manufacturing (AM) to revolutionize aspects of industrial production is widely recognized. AM can create objects with non-uniform properties by varying the ratio between deposited materials or altering the internal structure of the object. Amongst many possibilities, such AM objects could benefit the design and fabrication of different passive components of ultrasonic transducers, e.g. backing material, lenses and matching layers. The acoustic properties of AM objects produced using the Polyjet and Fused Deposition Methods were characterized. Initial results suggest that these technologies can easily produce objects with a wide range of tuned acoustic properties by varying either the internal structure or the material composition.

Modification of the ultrasonic properties of elastomers loaded with magnetic particles by applying magnetic fields during curing

Particle loaded polymers and elastomers have been largely used in different ultrasonic applications like backing materials and matching layers because composite properties (velocity, attenuation, density and impedance) can be easily engineered by changing the filler concentration. Recently, the use of magnetic particles has been theoretically proposed as a means to produce active matching layers whose response can be modified upon the application of magnetic fields. In this paper, we propose to introduce modifications of the composite properties by using magnetic particles and applying magnetic fields during curing to establish well defined patterns in the spatial distribution of the particles within the elastomer that induce material anisotropy that effectively modify ultrasonic properties.