Maria Gabriella Barbagallo

Berry size and qualitative characteristics of Vitis vinifera L. cv. Syrah

The effect of variation in berry size on berry composition was studied in irrigated Syrah/R99 grapevines, located in a temperate area of South Africa. Berries from 45 clusters, sampled from both sides of the canopy (east and west), were weighed to create four categories: 1. less or equal to 1.5 g; 2. between 1.51 g and 2.00 g; 3. between 2.01 g and 2.50 g; 4. more than 2.50 g. Berry physical characteristics were determined and total anthocyanins and seed flavonoids were analysed by spectrophotometry and anthocyanin profiles by HPLC. The ratio of skin weight:berry weight did not change with increasing berry size, but the ratio of seed weight:berry weight increased. For total anthocyanins, varying results were obtained if values were expressed in mg/berry, mg/g skin, mg/kg grape and mg/cm2 skin. The quantity of total anthocyanins changed positively with increasing berry weight if expressed in mg/berry and remained almost similar if expressed in mg/cm2 of skin. In contrast, total polyphenols decreased with berry weight if the values were expressed in mg/kg grape and in mg/g skin. Total anthocyanins expressed in mg/kg grape and in mg/g skin were correlated positively to the number of berries as well as to the total skin surface in one kilogram of grape. In this study, the largest berries seemed to have lower quality characteristics. In order to obtain better wine quality, it seems important to reduce berry weight and, in general, berry size variability of Syrah. The obtainment and continuity of a particular wine style may be affected by berry variability.

Characterization of carob germplasm (Ceratonia siliqua L.) in Sicily.

SummarySixteen carob cultivars were studied to evaluate pulp and seed yield with regard to endosperm percentage, to determine the overall degree of polymorphism of the characters and to detect similarities among cultivars. Bean size and numbers of normal and aborted seeds were characters with a certain degree of polymorphism. The low between-year variability observed suggests that differences in the variables could be attributed to genetic factors. A high year-to-year variation was observed only for the number of aborted seeds. Seed characteristics showed small changes both between years and among cultivars. Out of all the variables, PCA identified four principal components that explained more than 80% of the total variance. The first two principal components were related to qualitative characters and were useful in ranking cultivars. Plots of the principal components scores separated some cultivars for particular characteristics for industrial purposes.

Effect of artificial shading on the tannin accumulation and aromatic composition of the Grillo cultivar (Vitis vinifera L.)

BackgroundWhite wine quality, especially in warm climates, is affected by sunlight and heat stress. These factors increase the probability that ambering processes will occur and reduce the potential flavour compounds. This study aimed to investigate the effect of sunlight reduction on the accumulation of polyphenolic and aromatic compounds.ResultsThis study was conducted in a commercial vineyard containing V. vinifera L. cv Grillo. Opaque polypropylene boxes (100% shading) and high-density polyethylene (HDPE) net bags (50% shading) were applied at fruit set. The effect of the shaded treatments was compared to the exposed fruit treatment. The shaded treatments resulted in heavier berries and lower must sugar contents than the exposed treatments. Proanthocyanidins and total polyphenol levels were similar in the exposed and bagged grapes; however, the levels were always lower in the boxed fruit. At harvest, the highest aroma level was measured in the boxed fruits.ConclusionsThe boxed fruit had less sugar, fewer proanthocyanidins and more flavours than the exposed grapes.The reduction in flavanols reactive to p-dimethylamino-cinnamaldehyde as (+)-catechin equivalents and total skin proanthocyanidins is an important result for the white winemaking process. In addition, the higher level of aromatic compounds in shaded grapes at harvest is an important contribution to the development of different wine styles.

High-throughput 18K SNP array to assess genetic variability of the main grapevine cultivars from Sicily

The viticulture of Sicily, for its vocation, is one of the most important and ancient forms in Italy. Autochthonous grapevine cultivars, many of which known throughout the world, have always been cultivated in the island from many centuries. With the aim to preserve this large grapevine diversity, previous studies have already started to assess the genetic variability among the Sicilian cultivars by using morphological and microsatellite markers. In this study, simple sequence repeat (SSR) were utilized to verify the true-to-typeness of a large clone collection (101) belonging to 21 biotypes of the most 10 cultivated Sicilian cultivars. Afterwards, 42 Organization Internationale de la Vigne et du Vin (OIV) descriptors and a high-throughput single nucleotide polymorphism (SNP) genotyping array (Vitis18kSNP) were applied to assess genetic variability among cultivars and biotypes of the same cultivar. Ampelographic traits and high-throughput SNP genotyping platforms provided an accuracy estimation of genetic diversity in the Sicilian germplasm, showing the relationships among cultivars by cluster and multivariate analyses. The large SNP panel defined sub-clusters unable to discern among biotypes, previously classified by ampelographic analysis, belonging to each cultivar. These results suggested that a very large number of SNP did not cover the genome regions harboring few morphological traits. Genetic structure of the collection revealed a clear optimum number of groups for K = 3, clustering in the same group a significant portion of family-related genotypes. Parentage analysis highlighted significant relationships among Sicilian grape cultivars and Sangiovese, as already reported, but also the first evidences of the relationships between Nero d’Avola and both Inzolia and Catarratto. Finally, a small panel of highly informative markers (12 SNPs) allowed us to isolate a private profile for each Sicilian cultivar, providing a new tool for cultivar identification.

Berry Characterisation of cv Shiraz According to Position on the Rachis

In this study, characterisation of the physical and compositional parameters of berries located in different positions on the rachis of Shiraz/R99 bunches was done. Berries were divided according to position on the rachis (apical, median and basal) and berry weight, resulting in four berry weight classes, averaging 0.86 g, 1.29 g, 1.74 g and 2.26 g and 0.74, 1.18, 1.59 and 2.09 cm3, respectively. The berries were analysed individually. The fresh weight of the berries comprised approximately 4% seeds, 20% skin and 76% flesh. Different percentage distributions were found for each class of berry weight and according to position on the rachis. From the top to the bottom rachis position, an increase in skin proportion value and a decrease in flesh and seed proportion value occurred. Skins decreased and flesh increased in proportion from the smallest to the largest berry size. The seed maintained a relatively stable proportion, irrespective of berry size. Larger berries had more flesh compared to skin than smaller berries. A higher level of soluble solids occurred in the shoulder to middle bunch area (28.7 °B and 28.6 °B, respectively) than in the bottom area (27.6 °B). It is extremely difficult to obtain uniform berry size and composition under field conditions. Continuous efforts are required to reduce variation and the potential negative impact on wine quality and consistency of wine style.

Defoliation of two-wire vertical trellis: effect on grape quality

ABSTRACT Basal leaves were removed from Cabernet Sauvignon vines trained to a two-wire vertical trellis at fruit set and at veraison. Leaf removal did not modify total soluble solids and titratable acidity at harvest. Defoliation at fruit set of lower cordon recovered the grape anthocyanin composition gap between upper and lower cordons and that produced a positive effect on anthocyanin synthesis. Hence, control of the upper cordon and defoliation of fruit set of the lower cordon treatments showed comparable values of anthocyanins. Defoliation at veraison did not produce any appreciable effect. This study shows that skin anthocyanin composition in a two-wire vertical trellis can be modified by leaf removal in the fruit zone of the lower cordon, reducing variability in the ripeness between the two cordons.