Maria Giovanna Belcastro

A migration-driven model for the historical spread of leprosy in medieval Eastern and Central Europe

Leprosy was rare in Europe during the Roman period, yet its prevalence increased dramatically in medieval times. We examined human remains, with paleopathological lesions indicative of leprosy, dated to the 6th-11th century AD, from Central and Eastern Europe and Byzantine Anatolia. Analysis of ancient DNA and bacterial cell wall lipid biomarkers revealed Mycobacterium leprae in skeletal remains from 6th-8th century Northern Italy, 7th-11th century Hungary, 8th-9th century Austria, the Slavic Greater Moravian Empire of the 9th-10th century and 8th-10th century Byzantine samples from Northern Anatolia. These data were analyzed alongside findings published by others. M. leprae is an obligate human pathogen that has undergone an evolutionary bottleneck followed by clonal expansion. Therefore M. leprae genotypes and sub-genotypes give information about the human populations they have infected and their migration. Although data are limited, genotyping demonstrates that historical M. leprae from Byzantine Anatolia, Eastern and Central Europe resembles modern strains in Asia Minor rather than the recently characterized historical strains from North West Europe. The westward migration of peoples from Central Asia in the first millennium may have introduced different M. leprae strains into medieval Europe and certainly would have facilitated the spread of any existing leprosy. The subsequent decline of M. leprae in Europe may be due to increased host resistance. However, molecular evidence of historical leprosy and tuberculosis co-infections suggests that death from tuberculosis in leprosy patients was also a factor.

Environmental stress increases variability in the expression of dental cusps

Teeth are an important model for developmental studies but, despite an extensive literature on the genetics of dental development, little is known about the environmental influences on dental morphology. Here we test whether and to what extent the environment plays a role in producing morphological variation in human teeth. We selected a sample of modern human skulls and used dental enamel hypoplasia as an environmental stress marker to identify two groups with different stress levels, referred to as SG (stressed group) and NSG (nonstressed group). We collected data on the occurrence and the relative development of 15 morphological traits on upper molars using a standard methodology (ASU-DAS system) and then we compared the frequencies of the traits in the two groups. Overall, the results suggest that (a) stressors like malnutrition and/or systemic diseases have a significant effect on upper molar morphology; (b) stress generates a developmental response which increases the morphological variability of the SG; and (c) the increase in variability is directional, since individuals belonging to the SG have more developed and extra cusps. These results are consistent with the expectations of the current model of dental development.

Skeletal evidence of tuberculosis in a modern identified human skeletal collection (Certosa cemetery, Bologna, Italy)

The diagnosis of tuberculosis (TB) in osteoarcheological series relies on the identification of osseous lesions caused by the disease. The study of identified skeletal collections provides the opportunity to investigate the distribution of skeletal lesions in relation to this disease. The aim of this study was to examine the skeletal evidence for TB in late adolescent and adult individuals from the identified human collection of the Certosa cemetery of Bologna (Italy, 19th-20th c.). The sample group consists of 244 individuals (138 males, 106 females) ranging from 17 to 88 years of age. The sample was divided into three groups on the basis of the recorded cause of death: TB (N = 64), pulmonary non-TB (N = 29), and other diseases (N = 151). Skeletal lesions reported to be related to TB were analyzed. The vertebral lesions were classified into three types: enlarged foramina (EnF, vascular foramina with diameter of 3-5 mm), erosions (ER), and other foramina (OtF, cavities of various shapes > 3 mm). A CT scan analysis was also performed on vertebral bodies. Some lesions were seldom present in our sample (e.g., tuberculous arthritis). OtF (23.7%) and subperiosteal new bone formation on ribs (54.2%) are significantly more frequent in the TB group with respect to the other groups. The CT scan analysis showed that the vertebrae of individuals who have died of TB may have internal cavities in the absence of external lesions. These traits represent useful elements in the paleopathological diagnosis of TB.

Variation of skeletal degenerative joint disease features in an identified Italian modern skeletal collection

OBJECTIVESnThe objective of this study was to investigate the relationship of degenerative joint disease (DJD) features (marginal lipping, porosity, surface osteophytes, eburnation, and loss of joint morphology) with sex, age, and occupation.nnnMATERIALS AND METHODSnWe devised a recording method for scoring the single features in the shoulder, elbow, wrist, hip, knee, and ankle of an Italian skeletal sample of 332 individuals (malesu2009=u2009161, femalesu2009=u2009171) of known sex, age, and occupation from the Frassetto collection (Sassari, Sardinia, 19th-20th century).nnnRESULTSnOur data revealed that: (1) the frequency of all features increases with age; (2) porosity is the first feature to appear, being in general the most frequent feature in young adults; (3) sex differences are especially found in the upper limbs of mature and elderly adults; (4) marginal lipping and surface osteophytes show a lesser age correlation and, in general, higher frequencies in the left lower limb; (5) no differences were found among different occupations.nnnDISCUSSIONnWe explain these results in light of different metabolic and adaptive processes in relation to age and sex. Static (weight-bearing) rather than dynamic mechanical stress could play a pivotal role in the distribution of the features observed in the lower limbs. The considerable increase in marginal lipping and surface osteophytes in elderly adults may be an adaptation for sustaining mechanical loading, balancing the loss of bone tissue which is characteristic of ageing processes (osteopenia, osteoporosis). Am J Phys Anthropol, 2016.

Patterns of Irregular Burials in Western Europe (1st-5th Century A.D.)

Background Irregular burials (IB—burials showing features that contrast with the majority of others in their geographic and chronological context) have been the focus of archaeological study because of their relative rarity and enigmatic appearance. Interpretations of IB often refer to supposed fear of the dead or to social processes taking place in time-specific contexts. However, a comprehensive and quantitative analysis of IB for various geographical contexts is still lacking, a fact that hampers any discussion of these burials on a larger scale. Methods Here, we collected a bibliographic dataset of 375 IB from both Britain and Continental Europe, altogether spanning a time period from the 1st to the 5th century AD. Each burial has been coded according to ten dichotomous variables, further analyzed by means of chi-squared tests on absolute frequencies, non-metric multidimensional scaling, and cluster analysis. Results Even acknowledging the limits of this study, and in particular the bias represented by the available literature, our results point to interesting patterns. Geographically, IB show a contrast between Britain and Continental Europe, possibly related to historical processes specific to these regions. Different types of IB (especially prone depositions and depositions with the cephalic extremity displaced) present a series of characteristics and associations between features that permit a more detailed conceptualization of these occurrences from a socio-cultural perspective that aids to elucidate their funerary meaning. Conclusions and Significance Altogether, the present work stresses the variability of IB, and the need to contextualize them in a proper archaeological and historical context. It contributes to the discussion of IB by providing a specific geographic and chronological frame of reference that supports a series of hypotheses about the cultural processes possibly underlying their occurrence.

Dental status and 3D reconstruction of the malocclusion of the famous singer Farinelli (1705–1782)

The famous castrato singer Carlo Broschi, better known as Farinelli (1705-1782), was exhumed by our research group in July 2006 to study his skeletal remains and reconstruct his osteobiography. He was castrated before puberty to preserve his high voice into adulthood. The osteological study has revealed several skeletal features probably related to the effects of castration (Hyperostosis frontalis interna, long limb bones, persistence of epiphyseal lines, osteoporosis) (Belcastro et al., 2011). Here we present the study of the teeth and maxilla-mandibular region using classic and tomographic morphological methods. Considering the subjects age and the period during which he lived, his oral health conditions were good. On the basis of the very pronounced anomalous vestibular buccal wear, a overbite visualized by 3D reconstruction, was hypothesized. This facial disharmony is of particular interest when considering Farinellis extraordinary singing qualities and stage presence.

Unravelling biocultural population structure in 4th/3rd century BC Monterenzio Vecchio (Bologna, Italy) through a comparative analysis of strontium isotopes, non-metric dental evidence, and funerary practices

The 4th century BC marks the main entrance of Celtic populations in northern Italy. Their arrival has been suggested based on the presence of Celtic customs in Etruscan mortuary contexts, yet up to now few bioarchaeological data have been examined to support or reject the arrival of these newcomers. Here we use strontium isotopes, non-metric dental traits and funerary patterns to unravel the biocultural structure of the necropolis of Monterenzio Vecchio (Bologna, Italy). Subsamples of our total sample of 38 individuals were analyzed based on different criteria characterizing the following analyses: 1) strontium isotope analysis to investigate migratory patterns and provenance; 2) non-metric dental traits to establish biological relationships between Monterenzio Vecchio, 13 Italian Iron age necropolises and three continental and non-continental Celtic necropolises; 3) grave goods which were statistically explored to detect possible patterns of cultural variability. The strontium isotopes results indicate the presence of local and non-local individuals, with some revealing patterns of mobility. The dental morphology reveals an affinity between Monterenzio Vecchio and Iron Age Italian samples. However, when the Monterenzio Vecchio sample is separated by isotopic results into locals and non-locals, the latter share affinity with the sample of non-continental Celts from Yorkshire (UK). Moreover, systematic analyses demonstrate that ethnic background does not retain measurable impact on the distribution of funerary elements. Our results confirm the migration of Celtic populations in Monterenzio as archaeologically hypothesized on the basis of the grave goods, followed by a high degree of cultural admixture between exogenous and endogenous traits. This contribution shows that combining different methods offers a more comprehensive perspective for the exploration of biocultural processes in past and present populations.

From corpse to bones: funerary rituals of the Taforalt Iberomaurusian population

The funerary practices of the Taforalt population (Morocco, about 15-12500 Cal BP), already investigated by the authors in previous studies, were reconstructed through anthropological analyses of human remains (excavated by J. Roche in the 1950s) and examination of the available field documentation. The results indicated that the burial area included primary and secondary depositions, sometimes within the same grave, of approximately 40 adolescents and adults, as well as several children. There is evidence of treatment of the cadavers (disarticulation and defleshing) and manipulation of dry bones. The funerary practices at Taforalt involved a sequence of actions revealing a ritual character. These reflect the tripartite structure of Van Gennep’s rites of passage, implying that death was conceived as a passage into another social dimension.The aim of this study is to analyse the post mortem interventions on these human remains (corpses and bones) and to offer interpretative hypotheses as to their possible meaning, drawing on the findings of ethnographic research. We suggest that the collective dimension of the group was valued more highly than the members of the group as individuals, and that dry bones were imbued with symbolic meaning. We also suggest that the cultural and social management (through rites) of natural events (death), associated with a strengthening of the bonds with earth (inhumation) and territory (cemetery), may be interpreted as heralding the emergence of a Neolithic pattern of thought.RésuméLes pratiques funéraires de la population de Taforalt (Maroc, 15-12500 Cal BP) ont été étudiées par les auteurs dans des travaux précédents. La reconstitution de ces pratiques a été réalisée à partir de l’analyse anthropologique des restes humains (fouilles: J. Roche, années 1950) et de l’examen des documents disponibles de fouilles.Ces travaux suggèrent que la zone d’inhumation comprend des dépôts primaires et secondaires, parfois dans la même sépulture, d’environ 40 individus adolescents et adultes, ainsi que de plusieurs enfants. Le traitement du cadavre (désarticulation, décharnement) et autres manipulations sur os sec sont attestés. Le comportement funéraire à Taforalt rend compte d’une succession d’actions à caractère rituel. Ces rites étaient structurés comme des rites de passage, dans lesquels la mort était probablement vue comme un passage à une autre dimension sociale.Le but du présent article est d’analyser les interventions post mortem sur corps et os et de proposer une hypothèse d’interprétation de leur possible signification. En partant des recherches ethnographiques, nous proposons que la dimension collective du groupe était valorisée à la place de l’individu. Nous proposons que la gestion culturelle et sociale (à travers les rites) d’événements naturels (la mort), associée au renforcement des liens avec la terre (sépultures) et le territoire (inhumation) pourraient être perçues comme un changement dans la façon de penser annonçant le néolithique.