María Guadalupe Guerra-Sánchez
Instituto Politécnico Nacional
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Publication
Featured researches published by María Guadalupe Guerra-Sánchez.
Journal of Basic Microbiology | 2014
Leobarda Robles-Martínez; María Guadalupe Guerra-Sánchez; Ana Niurka Hernández-Lauzardo; Juan Pablo Pardo; Miguel G. Velázquez-del Valle
The antifungal activities of chitosan and oligochitosan have been used to control postharvest decay of the fruits. The effect of chitosan and oligochitosan on mycelium growth, spore germination, and mitochondrial function of Rhizopus stolonifer was evaluated in order to establish a connection between fungus development and the main organelle in charge to provide energy to the cell. The mycelium growth of R. stolonifer was significantly reduced on minimum media amended with chitosan or oligochitosan. The highest antifungal indexes were obtained on media containing chitosan or oligochitosan at 2.0 mg ml−1. Microscopic observation showed that chitosan and oligochitosan affected the spore germination and hyphae morphology. Both polymers increased oxygen consumption of R. stolonifer. Respiratory activity was restored with NADH in permeabilized treated and untreated cells, and was inhibited with rotenone and flavones. Complex III and IV were inhibited by antimycin A and cyanide, respectively, in treated and untreated cells. Chitosan and oligochitosan increased NADH dehydrogenase activity in isolated mitochondria. However, there were not changes in the cytochrome c oxidase and ATPase activities by effect of these polymers. These results suggest that both chitosan and oligochitosan affect the development of R. stolonifer and might be implicated in the mitochondrial dysfunction.
Archives of Microbiology | 2013
Leobarda Robles-Martínez; María Guadalupe Guerra-Sánchez; Oscar Flores-Herrera; Ana Niurka Hernández-Lauzardo; Miguel G. Velázquez-del Valle; Juan Pablo Pardo
Rhizopus stolonifer (Ehrenb.:Fr.) Vuill mitochondria contain the complete system for oxidative phosphorylation, formed by the classical components of the electron transport chain (complexes I, II, III, and IV) and the F1F0-ATP synthase (complex V). Using the native gel electrophoresis, we have shown the existence of supramolecular associations of the respiratory complexes. The composition and stoichiometry of the oxidative phosphorylation complexes were similar to those found in other organisms. Additionally, two alternative routes for the oxidation of cytosolic NADH were identified: the alternative NADH dehydrogenase and the glycerol-3-phosphate shuttles. Residual respiratory activity after inhibition of complex IV by cyanide was inhibited by low concentrations of n-octyl gallate, indicating the presence of an alternative oxidase. The K0.5 for the respiratory substrates NADH, succinate, and glycerol-3-phosphate in permeabilized cells was higher than in isolated mitochondria, suggesting that interactions of mitochondria with other cellular elements might be important for the function of this organelle.
Archives of Phytopathology and Plant Protection | 2012
Ana Niurka Hernández-Lauzardo; María Guadalupe Guerra-Sánchez; Annia Hernández-Rodríguez; Mayra Heydrich-Pérez; Josam Vega-Pérez; Miguel G. Velázquez-del Valle
The effect of chitosan was evaluated in three isolates of Rhizopus stolonifer from infected tomatoes that were harvested in different regions of the state of Morelos, México. Changes in the membrane integrity of spores, modifications in pH media and the proteins released were analysed in each isolate against three different molecular weights of chitosan at three fixed concentrations. There was an observed decline in the integrity of spores in the presence of chitosan at all the concentrations evaluated. The protein released was different depending on the isolate, kind of chitosan and used concentration. There was a significant difference in the pH changes on the growth media for all the tested isolates. Chitosan of high molecular weight showed the best results to inhibit the infection caused by R. stolonifer on the tomato fruits. The severity of symptoms of soft rot was not related with the molecular weight of chitosan.
Pesticide Biochemistry and Physiology | 2009
María Guadalupe Guerra-Sánchez; Josam Vega-Pérez; M.G. Velázquez-del Valle; Ana Niurka Hernández-Lauzardo
Pesticide Biochemistry and Physiology | 2010
J. García-Rincón; Josam Vega-Pérez; María Guadalupe Guerra-Sánchez; Ana Niurka Hernández-Lauzardo; A. Peña-Díaz; M.G. Velázquez-del Valle
Fruits | 2010
Ana Niurka Hernández-Lauzardo; Miguel G. Velázquez-del Valle; Leticia Veranza-Castelán; Gloria Elena Melo-Giorgana; María Guadalupe Guerra-Sánchez
Revista mexicana de fitopatología | 2007
Ana Niurka Hernández-Lauzardo; Maricruz Hernández-Martínez; Miguel G. Velázquez-del Valle; María Guadalupe Guerra-Sánchez; Gloria Elena Melo-Giorgana
Revista Mexicana de Fitopatología | 2008
Miguel G. Velázquez-del Valle; Silvia Bautista-Baños; Ana Niurka Hernández-Lauzardo; María Guadalupe Guerra-Sánchez; Enriqueta Amora-Lazcano
Journal of Phytopathology | 2014
Ivonne C. Alfaro-Gutiérrez; María Guadalupe Guerra-Sánchez; Ana Niurka Hernández-Lauzardo; Miguel G. Velázquez-del Valle
Revista Colombiana de Biotecnología | 2010
María Guadalupe Guerra-Sánchez; Luis Armando Sandoval-Escobar; Enriqueta Amora-Lazcano; Luis Vásquez-Méndez; Miguel G. Velázquez-del Valle; Ana Niurka Hernández-Lauzardo