Maria Laura Colombo

An Update on Vitamin E, Tocopherol and Tocotrienol—Perspectives

Vitamin E, like tocotrienols and tocopherols, is constituted of compounds essential for animal cells. Vitamin E is exclusively synthesized by photosynthetic eukaryotes and other oxygenic photosynthetic organisms such as cyanobacteria. In order to prevent lipid oxidation, the plants mainly accumulate tocochromanols in oily seeds and fruits or in young tissues undergoing active cell divisions. From a health point of view, at the moment there is a great interest in the natural forms of tocochromanols, because they are considered promising compounds able to maintain a healthy cardiovascular system and satisfactory blood cholesterol levels. Some evidence suggests that the potency of the antioxidant effects may differ between natural or synthetic source of tocochromanols (vitamin E).

Cytotoxicity evaluation of natural coptisine and synthesis of coptisine from berberine

The crude extract (80% MeOH in water) of Chelidonii herba exhibited very interesting cytotoxicity against brine shrimp (Artemia salina Leach) nauplii and cultured human tumour cell in vitro, the colon carcinoma HT 29 (144 h treatment). Fractionation of the crude extract and bioassay-guided procedures showed that the cytotoxic and the antitumour activities were concentrated in the basic extract. On the basis of IR, MS and 1H NMR the compound responsible of the cytotoxic activity was determined to be coptisine. Cytotoxicity evaluation of coptisine was next extended to a panel of human and murine cell lines in comparison with the established antitumour drugs mitoxantrone, doxorubicin (Dx) and cisplatin (CDDP). Coptisine was cytotoxic on LoVo and HT 29 and less potent on L-1210, and it was partially crossresistant on the human tumour colon cell line resistant to Dx, LoVo/Dx, whereas it was not significantly crossresistant on the murine leukaemia cell line resistant to CDDP, L-1210/CDDP. Coptisine alkaloid was then synthesised in gram amount from commercial berberine. A four-step synthetic route was elaborated. The overall yield was about 8-10%. The structural identity of synthetic coptisine was verified by IR and NMR methods. A comparison of the cytotoxic effects on the human tumour colon cell line LoVo and on the murine leukaemia L1210 showed, for both natural and synthetic coptisines, a comparable cytotoxic activity more evident against HT 29 cell line and LoVo cell line, while the activity was lower against the L1210 cell line.

Determination of the active principles of Catha Edulis: Quali–quantitative analysis of cathinone, cathine, and phenylpropanolamine

In the last years, all the vegetable material supposed to belong to the Catha Edulis species, seized at the Malpensa and Orio al Serio airports, were analyzed in our laboratory on behalf of the Tribunals of Busto Arsizio and Bergamo, respectively. After a preliminary botanic examination, the quali-quantitative determination of the active principles cathinone, cathine and phenylpropanolamine (PPA) was carried out by means of GC/MS and GC/FID techniques, which meet the requirements of the forensic analyses. We developed a fast, effective and reliable derivatization procedure which allowed to simultaneously detect cathine and PPA, whose discrimination is mandatory since PPA is not a psychoactive agent. Cathine was distributed in the various parts of the plant (leaves and stems) and its quantity ranged from 0.03% to 0.17% of the weight of the vegetable material; PPA was not detected in the twigs and its quantity in the leaves ranged from 0.07% to 0.16%. The quantitative determination of cathinone was carried out directly on the methanol solution after maceration of the vegetable material, its quantity ranging from 0.02% to 0.10%. No significant difference in the content of the two active principles was found between the fresh and the dried material.

Metal Content in Dandelion (Taraxacum officinale) Leaves: Influence of Vehicular Traffic and Safety upon Consumption as Food

The widespread distribution of the common dandelion, that is, Taraxacum officinale, along with its ability to tolerate a wide range of environmental conditions, makes this plant a good candidate as biological monitor of environmental metal contamination. Taraxacum officinale leaves growing spontaneously in meadows and along the streets are traditionally picked up and eaten in Italy as salad, so it is important to know the concentrations of potentially toxic elements contained in them from the point of view of food safety. For these reasons the concentrations of Cd, Cr, Cu, Fe, Mn, Pb, and Zn were determined in dandelion leaf and underlying soil samples collected at 12 sites in the province of Cuneo (Piedmont, Italy) in the vicinity of streets or roundabouts. The concentrations were compared with reference values for plant and soils and with maximum allowable concentrations in edible vegetables. Neither dandelion nor soil samples were found to be polluted by metals, but the comparison with limits for vegetables suggests that caution should be used in consuming spontaneously growing vegetables.

Food safety and nutritional quality for the prevention of non communicable diseases: the Nutrient, hazard Analysis and Critical Control Point process (NACCP)

BackgroundThe important role of food and nutrition in public health is being increasingly recognized as crucial for its potential impact on health-related quality of life and the economy, both at the societal and individual levels. The prevalence of non-communicable diseases calls for a reformulation of our view of food. The Hazard Analysis and Critical Control Point (HACCP) system, first implemented in the EU with the Directive 43/93/CEE, later replaced by Regulation CE 178/2002 and Regulation CE 852/2004, is the internationally agreed approach for food safety control. Our aim is to develop a new procedure for the assessment of the Nutrient, hazard Analysis and Critical Control Point (NACCP) process, for total quality management (TMQ), and optimize nutritional levels.MethodsNACCP was based on four general principles: i) guarantee of health maintenance; ii) evaluate and assure the nutritional quality of food and TMQ; iii) give correct information to the consumers; iv) ensure an ethical profit. There are three stages for the application of the NACCP process: 1) application of NACCP for quality principles; 2) application of NACCP for health principals; 3) implementation of the NACCP process. The actions are: 1) identification of nutritional markers, which must remain intact throughout the food supply chain; 2) identification of critical control points which must monitored in order to minimize the likelihood of a reduction in quality; 3) establishment of critical limits to maintain adequate levels of nutrient; 4) establishment, and implementation of effective monitoring procedures of critical control points; 5) establishment of corrective actions; 6) identification of metabolic biomarkers; 7) evaluation of the effects of food intake, through the application of specific clinical trials; 8) establishment of procedures for consumer information; 9) implementation of the Health claim Regulation EU 1924/2006; 10) starting a training program.Results and discussionWe calculate the risk assessment as follows: Risk (R) = probability (P) × damage (D). The NACCP process considers the entire food supply chain “from farm to consumer”; in each point of the chain it is necessary implement a tight monitoring in order to guarantee optimal nutritional quality.

Analysis of food supplements containing iodine: a survey of Italian market

Iodine is an essential nutrient for humans as a critical constituent of thyroid hormones; both iodine deficiency and excess have adverse consequences for the thyroid gland. The aims of this research were to compare claimed concentrations of iodine with measured ones in various iodine-supplemented products, estimate the amount of iodine ingested by Italian consumers who use these products, and compare the calculated intakes to the Recommended Daily Allowance and tolerable Upper Level. A convenience sample of 43 food supplements was analyzed for iodine concentration. Analytical values resembled those declared in the label in fewer than half of the examples; in four cases, the maximal daily dose was higher than the tolerable upper level for iodine of 600 μg/day. Labeling of iodine-rich food supplements appears to be unreliable and caution should be exercised in the consumption of food supplements rich in iodine as there is a risk of exceeding the established safe upper level of daily intake.

Lipid content and fatty acid composition in hypogeous organs ofHelleborus species (Ranunculaceae)

Root and rhizome of fourHelleborus species:H. viridis, H. odorus, H. niger, H. foetidus were examined throughout a two-year period in order to determine the variation of lipid content and fatty acid composition during the ontogenetic cycle. In the deciduous geophytesH. viridis andH. odorus subsp.laxus the lipid content reaches its maximum during the quiescent phase, whereas in the evergreen geophyteH. niger the lipid content is highest during the main growth period. In the chamaephyteH. foetidus lipid content is always very low and it does not show variation throughout the year. In all species the fatty acids detected in the neutral fraction are myristic, palmitic, stearic, oleic and linoleic acid. Palmitic and linoleic acid are always present in larger quantities. The pattern of the relative content of saturated/unsaturated fatty acids seems to be linked to the life form of these species.

Analysis of fluid extracts obtained from Papaver rhoeas petals contaminated with Papaver bracteatum petals.

In this paper, we report a case of misidentification of medicinal plants involving dried petals of Papaver rhoeas (red poppy) contaminated with Papaver bracteatum (scarlet poppy) petals. Preliminary TLC analysis indicated the presence of thebaine either in the fluid extracts or in the petals. It was therefore necessary to carry out an accurate botanic examination of the plant material, which revealed contamination of the red poppy petals with scarlet poppy petals. Moreover, to confirm the adulteration, we developed and validated an efficient, reversed-phase ion pair HPLC method for determination of the alkaloids specific for the Papaver species. Six petal batches and five commercial fluid extracts were analyzed. Only one petal batch from Iran contained thebaine and its analogue oripavine while the alkaloids typical for the Papaver bracteatum species were identified in all fluid extracts, meaning that they were all prepared with contaminated petals.

Botany and Pharmacognosy of the Cacao Tree

Cacao trees originated in river valleys of South America and, by the seventh century AD, the Mayan Indians had brought them north into Mexico. Apart from the Mayans, many other Central American Indians including the Aztecs and the Toltecs seem to have at first domesticated and then cultivated cacao trees, and the word “chocolate” (the beverage) derives from xhocolatl (approximate spelling) or cacahuatl, both originating from the Aztec language. There are several mixtures of cacao described in ancient texts, for ceremonial, medicinal and culinary purposes. Some mixtures included maize, chili, vanilla (Vanilla planifolia), peanut butter and honey. Archaeological evidence of the use of cacao, while relatively sparse, has come from the recovery of whole cacao beans in Uaxactun, Guatemala and from the preservation of wood fragments of the cacao tree at the Belize sites (ex British Honduras). In addition, analysis of residues from ceramic vessels has found traces of theobromine and caffeine in early formative vessels from Puerto Escondido, Honduras (1100–900 BC) and in middle formative vessels from Colha, Belize (600–400 BC) [1], [2].

Characterization of a suspension culture of Chelidonium majus L. on growth and accumulation of sanguinarine

Summary Cell suspension cultures of C. majus L. — obtained from callus — were characterized on growth and on accumulation of isoquinoline alkaloids. The liquid batch cultures consisted of coarse cell aggregates; their fresh volume becames triple each 14 days after subcultures. Cell cultures were found to produce relatively large amounts of isoquinoline alkaloids; sanguinarine is accumulated as main alkaloid and also released into the medium.