María Laura Silveira

Role of the amygdala and periaqueductal gray in anxiety and panic

The amygdala (AM) and the periaqueductal gray (PAG) represent the rostral and the caudal pole, respectively, of a longitudinally organized neural system, that is responsible for the integration of behavioral and physiological manifestations of defensive reactions against innate and learned threats. Microinjection of benzodiazepine (BZD) anxiolytics, GABAA receptor agonists or 5-HT receptor antagonists into the AM has anxiolytic effects in conflict tests and other models of conditioned fear, while similar administration of 5-HT or of a 5-HT1A receptor agonist has anxiogenic effects. On the other hand, in the test of electrical stimulation of the PAG, microinjection of 5-HT, 5-HT mimetics, or of drugs that enhance the action of endogenous 5-HT into the same brain area has an antiaversive effect, like BZD and GABAA agonists. Furthermore, microinjection of midazolam, of the NMDA receptor antagonist AP-7, or of the 5-HT1A/1B receptor blocker propranolol increased the exploration of the open arms of the elevated plus-maze, having therefore an anxiolytic effect. These results point to an inhibitory role of the GABA-BZD system in both the AM and the PAG. In contrast, 5-HT seemingly enhances conditioned fear in the AM, while inhibiting unconditioned fear in the PAG. Thus, 5-HT2/1C antagonists reportedly release punished behavior when injected into the AM, whereas they antagonized the antiaversive effect of 5-HT, zimelidine and 5-HT1A/1B receptor blockers in the PAG. Since reported clinical studies revealed that one of such compounds, ritanserin, relieves generalized anxiety but tends to aggravate panic disorder, a relationship may be established between the AM and anxiety and the PAG and panic.

Induction of Fos immunoreactivity in the brain by exposure to the elevated plus-maze

The Fos protein immunohistochemistry technique was used to map the brain areas activated by a 15-min exposure of rats to the elevated plus maze, an ethologically based animal model of anxiety. Two hours after the test, labeling was found mainly in the piriform and entorhinal cortices, amygdala, midline thalamic nuclei, several medial hypothalamic nuclei, periaqueductal gray matter, superior and inferior colliculus, cuneiform nucleus, dorsal raphe nucleus and locus coeruleus. These results support a participation of these structures in anxiety.

Defense reaction elicited by microinjection of kainic acid into the medial hypothalamus of the rat: Antagonism by a GABAA receptor agonist

Electrical stimulation of either the midbrain central gray or the medial hypothalamus induces a defense reaction in the rat, characterized mainly by increased locomotion, rearing, and leaping. However, microinjection of the excitatory amino acid glutamate was effective only in the former region. Because excitatory amino acids do not depolarize axons of passage, it was suggested that the hypothalamus is devoid of soma/dendrites of neurons commanding the defense reaction. In the present study, we show that a subtoxic dose (60 pmol) of another excitatory amino acid, kainic acid, injected into the medial hypothalamus significantly enhanced locomotion and rearing of Wistar rats systematically observed in an open field. Similar behavioral changes have been reported following microinjection of drugs impairing GABAergic neurotransmission. Local pretreatment with the GABAA receptor agonist THIP (2 nmol) blocked the effect of kainic acid. Therefore, the medial hypothalamus of the rat seems to contain a population of neuronal cell bodies commanding the defense reaction, which is activated by excitatory amino acids and tonically inhibited by GABAergic fibers.

Finanças, consumo e circuitos da economia urbana na cidade de São Paulo

Partindo do novo papel financeiro e organizacional da cidade de Sao Paulo, buscamos compreender as interferencias do circuito superior sobre o circuito inferior. Ambos os circuitos diferenciam-se pelos graus de capital, tecnologia e organizacao. Existe uma oferta extraordinaria de credito desburocratizado, realizada por instituicoes financeiras bancarias e nao-bancarias, redes, franquias e outlet de eletrodomesticos, roupas e materiais de construcao A complexa organizacao financeira do circuito superior, apoiada nos atuais sistemas tecnicos e na propaganda, permite a expansao social e territorial dos seus mercados, evitando capacidades ociosas e invadindo os mercados tradicionalmente pertencentes ao circuito inferior. Ainda que se trate de um fenomeno nacional, sua densidade, na metropole paulista, e indiscutivel, com a maior concentracao de estabelecimentos e dinheiro. Criam-se novos nexos de subordinacao, e as areas mais concentradas da metropole tornam-se localizacoes necessarias para essas grandes empresas. Aumentam, concomitantemente, o consumo e a pobreza.