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Dive into the research topics where Mariagrazia Marziano is active.

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Featured researches published by Mariagrazia Marziano.


Oxidative Medicine and Cellular Longevity | 2017

Nutrition and AGE-ing: Focusing on Alzheimer’s Disease

Giulia Abate; Mariagrazia Marziano; Wiramon Rungratanawanich; Maurizio Memo; Daniela Uberti

Recently, the role of food and nutrition in preventing or delaying chronic disability in the elderly population has received great attention. Thanks to their ability to influence biochemical and biological processes, bioactive nutrients are considered modifiable factors capable of preserving a healthy brain status. A diet rich in vitamins and polyphenols and poor in saturated fatty acids has been recommended. In the prospective of a healthy diet, cooking methods should be also considered. In fact, cooking procedures can modify the original dietary content, contributing not only to the loss of healthy nutrients, but also to the formation of toxins, including advanced glycation end products (AGEs). These harmful compounds are adsorbed at intestinal levels and can contribute to the ageing process. The accumulation of AGEs in ageing (“AGE-ing”) is further involved in the exacerbation of neurodegenerative and many other chronic diseases. In this review, we discuss foods dual role as both source of bioactive nutrients and reservoir for potential toxic compounds—paying particular attention to the importance of proper nutrition in preventing/delaying Alzheimers disease. In addition, we focus on the importance of a good education in processing food in order to benefit from the nutritional properties of an optimal diet.


Current Alzheimer Research | 2016

Comparison of Extracellular and Intracellular Blood Compartments Highlights Redox Alterations in Alzheimer's and Mild Cognitive Impairment Patients

Noemí Arce-Varas; Giulia Abate; Chiara Prandelli; Carmen Martinez; Fernando Cuetos; Manuel Menéndez; Mariagrazia Marziano; David Cabrera-García; Maria Teresa Fernández-Sánchez; Antonello Novelli; Maurizio Memo; Daniela Uberti

BACKGROUND Many studies suggest oxidative stress as an early feature of Alzheimers Disease (AD). However, evidence of established oxidative stress in AD peripheral cells is still inconclusive, possibly due to both, differences in the type of samples and the heterogeneity of oxidative markers used in different studies. OBJECTIVE The aim of this study was to evaluate blood-based redox alterations in Alzheimers Disease in order to identify a peculiar disease profile. METHOD To that purpose, we measured the activity of Superoxide Dismutase, Catalase and Glutathione Peroxidase both in the extracellular and the intracellular blood compartments of AD, MCI and control subjects. The amount of an open isoform of p53 protein (unfolded p53), resulting from oxidative modifications was also determined. RESULTS Decreased SOD, increased GPx activity and higher p53 open isoform were found in both AD and MCI plasma compared to controls. In blood peripheral mononuclear cells, SOD activity was also decreased in both AD and MCI, and unfolded p53 increased exquisitely in younger AD males compared to controls. CONCLUSION Overall, these data highlight the importance of considering both extracellular and intracellular compartments, in the determination of antioxidant enzyme activities as well as specific oxidation end-products, in order to identify peculiar blood-based redox alterations in AD pathology.


Oxidative Medicine and Cellular Longevity | 2018

Characterization of the Antioxidant Effects of γ-Oryzanol: Involvement of the Nrf2 Pathway

Wiramon Rungratanawanich; Giulia Abate; M. M. Serafini; M. Guarienti; M. Catanzaro; Mariagrazia Marziano; Maurizio Memo; Cristina Lanni; Daniela Uberti

γ-Oryzanol (ORY) is well known for its antioxidant potential. However, the mechanism by which ORY exerts its antioxidant effect is still unclear. In this paper, the antioxidant properties of ORY were investigated for its potential effects as a reactive oxygen and nitrogen species (ROS/RNS) scavenger and in activating antioxidant-promoting intracellular pathways utilizing the human embryonic kidney cells (HEK-293). The 24 h ORY exposure significantly prevented hydrogen peroxide- (H2O2-) induced ROS/RNS production at 3 h, and this effect was sustained for at least 24 h. ORY pretreatment also enhanced the activity of antioxidant enzymes: superoxide dismutase (SOD) and glutathione peroxidase (GPX). Interestingly, ORY induced the nuclear factor (erythroid-derived 2)-like 2 (Nrf2) nuclear translocation and upregulation of Nrf2-dependent defensive genes such as NAD(P)H quinone reductase (NQO1), heme oxygenase-1 (HO-1), and glutathione synthetase (GSS) at mRNA and protein levels in both basal condition and after H2O2 insult. Thus, this study suggested an intriguing effect of ORY in modulating the Nrf2 pathway, which is also involved in regulating longevity as well as age-related diseases.


Free Radical Biology and Medicine | 2018

Protein nitration profile of CD3+ lymphocytes from Alzheimer disease patients: Novel hints on immunosenescence and biomarker detection

Antonella Tramutola; Giulia Abate; Chiara Lanzillotta; Francesca Triani; Eugenio Barone; Federica Iavarone; Federica Vincenzoni; Massimo Castagnola; Mariagrazia Marziano; Maurizio Memo; Emirena Garrafa; D. Allan Butterfield; Marzia Perluigi; Fabio Di Domenico; Daniela Uberti

&NA; Alzheimers disease (AD) is a progressive form of dementia characterized by increased production of amyloid‐&bgr; plaques and hyperphosphorylated tau protein, mitochondrial dysfunction, elevated oxidative stress, reduced protein clearance, among other. Several studies showed systemic modifications of immune and inflammatory systems due, in part, to decreased levels of CD3+ lymphocytes in peripheral blood in AD. Considering that oxidative stress, both in the brain and in the periphery, can influence the activation and differentiation of T‐cells, we investigated the 3‐nitrotyrosine (3‐NT) proteome of blood T‐cells derived from AD patients compared to non‐demented (ND) subjects by using a proteomic approach. 3‐NT is a formal protein oxidation and index of nitrosative stress. We identified ten proteins showing increasing levels of 3‐NT in CD3+ T‐cells from AD patients compared with ND subjects. These proteins are involved in energy metabolism, cytoskeletal structure, intracellular signaling, protein folding and turnover, and antioxidant response and provide new insights into the molecular mechanism that impact reduced T‐cell differentiation in AD. Our results highlight the role of peripheral oxidative stress in T‐cells related to immune‐senescence during AD pathology focusing on the specific targets of protein nitration that conceivably can be suitable to further therapies. Further, our data demonstrate common targets of protein nitration between the brain and the periphery, supporting their significance as disease biomarkers. Graphical abstract Figure. No caption available. HighlightsAD is characterized by modifications of the immune systems associated with reduced T‐cells activity.We identified increased protein nitration levels in T‐cells from AD patients compared to healthy subjects.The protein showing increased nitration are involved in pathways crucial to maintain neuronal homeostasis.Our data provide new insights into the molecular mechanisms that lead to reduced T‐cell differentiation in AD.The finding of common nitration targets between the brain and the periphery support their use in disease prediction.


9th International Conference on Times of Polymers and Composites: From Aerospace to Nanotechnology | 2018

Shape memory electrospun nonwovens based on crosslinked poly(ε-caprolactone) for multifunctional biological applications

Nicoletta Inverardi; Paola S. Ginestra; Rosalba M. Ferraro; Sarah Tonello; Mariagrazia Marziano; Andrea Merlettini; Chiara Gualandi

In this work we have explored the capabilities of an electrospun mat, realized in crosslinked poly(e-caprolactone), to be employed as a scaffold for specific biological applications. Its shape memory behavior was here exploited to easily control fiber orientation and to guide cellular alignment. Randomly oriented mats were transformed in a stable shape with various degrees of fiber alignment by simply varying the maximum strain applied throughout proper thermo-mechanical cycles. The effect of fiber alignment was investigated both on mechanical properties and on cell culture, through biological characterization done by using Neural Stem Cells derived from human iPSCs (induced Pluripotent Stem Cells). Further, thanks to the thermal stability of the microfibrous structure, it was possible to transfer, through inkjet printing on the electrospun, a biocompatible sensing element, which successfully allowed to monitor cell adhesion (L6 Myoblast) in an innovative way under dynamic conditions.In this work we have explored the capabilities of an electrospun mat, realized in crosslinked poly(e-caprolactone), to be employed as a scaffold for specific biological applications. Its shape memory behavior was here exploited to easily control fiber orientation and to guide cellular alignment. Randomly oriented mats were transformed in a stable shape with various degrees of fiber alignment by simply varying the maximum strain applied throughout proper thermo-mechanical cycles. The effect of fiber alignment was investigated both on mechanical properties and on cell culture, through biological characterization done by using Neural Stem Cells derived from human iPSCs (induced Pluripotent Stem Cells). Further, thanks to the thermal stability of the microfibrous structure, it was possible to transfer, through inkjet printing on the electrospun, a biocompatible sensing element, which successfully allowed to monitor cell adhesion (L6 Myoblast) in an innovative way under dynamic conditions.


Archive | 2017

Enhanced Sensing of Interleukin 8 by Stripping Voltammetry: Carbon Nanotubes versus Fullerene

Sarah Tonello; Mariagrazia Marziano; Giulia Abate; Tugba Kilic; Maurizio Memo; Daniela Uberti; Sandro Carrara; N. Lopomo; Mauro Serpelloni; Emilio Sardini

The ability to detect protein biomarkers at a sub-nanomolar level represents a pervasive challenge in order to bring a significant improvement in early diagnosis or progression of pato-physiological processes. To this aim, Screen Printed Electrochemical Sensors have been acquiring a predominant importance. The possibility to use them with different measurement techniques, and to customize their surface to improve the performance represent really attractive features. In this work, performances of two different carbon nanostructures in combination with Stripping Voltammetry were evaluated as tools to improve the detection of Interleukin 8, a cytokine that has pivotal roles in various inflammatory processes and considered as a universal biomarker. Commercially-available Carbon sensors were modified using Carbon Nanotubes and Spherical Fullerene through drop casting technique. Interleukin 8 was quantified using an indirect techniques based on silver stripping catalyzed using Alkaline Phosphatase. The nanostructured sensors showed better sensitivity with sub-nanomolar limit of detection: 0.39 ng/ml for carbon nanotubes and 0.61 ng/ml for fullerene compared to bare carbon electrodes. These modification method is promising for sensitive detection of protein biomarkers in several applications, including the monitoring of inflammatory processes.


Archive | 2017

Carbon on poly(ε-caprolactone) (PCL) Ink-jet Printed Sensor for Monitoring Cell Cultures of Myoblasts

Mariagrazia Marziano; Sarah Tonello; Marialaura Serzanti; Michela Borghetti; N. Lopomo; Mauro Serpelloni; Stefano Pandini; Andrea Merlettini; Chiara Gualandi; Maria Letizia Focarete; Massimo Messori; Maurizio Toselli; Daniela Uberti; Maurizio Memo; Patrizia Dell’Era; Emilio Sardini

Nowadays techniques for sensitive non-invasive, real-time monitoring of cell differentiation and maturation are highly demanded. In light of this, the development of electrochemical printed sensors impedance-based could represent a promising tool. In the present work, we developed 2D ink-jet printed sensors for myoblasts adhesion monitoring, using carbon-based ink on a substrate consisting in non-woven electrospun mats made in crosslinked poly(e-caprolactone) (PCL). First of all, sensors printability was optimized and the biocompatibility tested. In order to determine the possibility to employ the prepared systems as scaffolds for dynamic cellular cultures, the mechanical response of the PCL scaffold was evaluated through the application of cyclic deformation tests. After that, electrical characterization of ink and substrate was performed, followed by electrochemical impedance-based measurements to evaluate myoblasts adhesion. Biocompatibility assessment showed good results for both carbon and PCL. Mechanical tests findings suggested that a training of 50 cycles and a proper value of strain should be applied before the cell seeding, in order to ensure a subsequent controlled strain amplitude. The sensorized scaffold allowed us to correlate cell adhesion with an increase of impedance module, in agreement with biocompatibility testing. Thus, this first preliminary testing suggested that this non-invasive impedance spectroscopy-based measurement system can be used for sensitive monitoring of cells adhesion, in static and moreover, as suggested from mechanical characterization, in dynamic conditions.


IEEE Transactions on Instrumentation and Measurement | 2017

Wireless Point-of-Care Platform With Screen-Printed Sensors for Biomarkers Detection

Sarah Tonello; Giulia Abate; Michela Borghetti; Mariagrazia Marziano; Mauro Serpelloni; Daniela Uberti; N. Lopomo; Maurizio Memo; Emilio Sardini

Measurement systems for early and reliable detection of degenerative diseases, such as Alzheimer’s disease (AD), are extremely important in clinical diagnosis. Among these, biochemical assays represent a commonly used method to distinguish patients from healthy population thanks to the sensitive recognition of specific biomarkers in biological fluids. In order to overcome actual limitations of these techniques in term of cost, standardization, and sensitivity, this study aimed to realize a low-cost highly sensitive portable point-of-care (PoC) testing system based on screen-printed electrochemical sensors. The development of the platform specifically included both the design of the sensing probe and the electronic circuit devoted to condition and acquires the transduced electric signal. The designed circuit was implemented in a printed circuit board and interfaced to a wireless system based on bluetooth data transmission in order to improve the portability of the proposed solution. Preliminary results were obtained by using controlled concentrations of electrolytic solutions and calibrating the sensors for antibodies and for a well-known protein (i.e., interleukin 8) quantified by anodic stripping voltammetry (ASV). Findings from ASV measurements showed a sensitivity of


Alzheimers & Dementia | 2017

A NEW PUTATIVE EARLY BIOMARKER FOR A BLOOD-BASED TIMELY DIAGNOSIS FOR ALZHEIMER’S DISEASE

Giulia Abate; Marika Vezzoli; Mariagrazia Marziano; Noemí Arce Varas; Wiramon Rungratanawanich; Carmen Martinez; Fernando Cueto; Maria Teresa Fernández-Sánchez; Antonello Novelli; Letizia Polito; Antonio Guaita; Maurizio Memo; Daniela Uberti

38~\mu \text{A}


Alzheimers & Dementia | 2016

AN OPEN ISOFORM OF P53 AS AN EARLY BIOMARKER OF BLOOD REDOX ALTERATIONS IN ALZHEIMER’S DISEASE: DEVELOPMENT OF AN EASY AND REPRODUCIBLE ASSAY

Giulia Abate; Noemí Arce Varas; Carmen Martinez; Fernando Cueto; Mariagrazia Marziano; Maria Teresa Fernández-Sánchez; Antonello Novelli; Maurizio Memo; Daniela Uberti

/(ng/ml) with a tested range from 1.25 to 20 ng/ml, with a limit of detection of 2 ng/ml. Further investigation will include the validation of this PoC device by testing the concentration of a specific p53 protein isoform, which was recently identified to early correlate to AD development.

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Carmen Martinez

Complutense University of Madrid

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N. Lopomo

University of Brescia

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