Mariana Peñuela

Model-based identifiable parameter determination applied to a simultaneous saccharification and fermentation process model for bio-ethanol production

In this work, a methodology for the model‐based identifiable parameter determination (MBIPD) is presented. This systematic approach is proposed to be used for structure and parameter identification of nonlinear models of biological reaction networks. Usually, this kind of problems are over‐parameterized with large correlations between parameters. Hence, the related inverse problems for parameter determination and analysis are mathematically ill‐posed and numerically difficult to solve. The proposed MBIPD methodology comprises several tasks: (i) model selection, (ii) tracking of an adequate initial guess, and (iii) an iterative parameter estimation step which includes an identifiable parameter subset selection (SsS) algorithm and accuracy analysis of the estimated parameters. The SsS algorithm is based on the analysis of the sensitivity matrix by rank revealing factorization methods. Using this, a reduction of the parameter search space to a reasonable subset, which can be reliably and efficiently estimated from available measurements, is achieved. The simultaneous saccharification and fermentation (SSF) process for bio‐ethanol production from cellulosic material is used as case study for testing the methodology. The successful application of MBIPD to the SSF process demonstrates a relatively large reduction in the identified parameter space. It is shown by a cross‐validation that using the identified parameters (even though the reduction of the search space), the model is still able to predict the experimental data properly. Moreover, it is shown that the model is easily and efficiently adapted to new process conditions by solving reduced and well conditioned problems.

Automatic identification of Scenedesmus polymorphic microalgae from microscopic images

Microalgae counting is used to measure biomass quantity. Usually, it is performed in a manual way using a Neubauer chamber and expert criterion, with the risk of a high error rate. Scenedesmus algae can build coenobia consisting of 1, 2, 4 and 8 cells. The amount of algae of each coenobium helps to determine the amount of lipids, proteins, and other substances in a given sample of a algae crop. The knowledge of the quantity of those elements improves the quality of bioprocess applications. This paper addresses the methodology for automatic identification of Scenedesmus microalgae (used in the methane production and food industry) and applies it to images captured by a digital microscope. The use of contrast adaptive histogram equalization for pre-processing, and active contours for segmentation are presented. The calculation of statistical features (histogram of oriented gradients, Hu and Zernike moments) with texture features (Haralick and local binary patterns descriptors) are proposed for algae characterization. Classification of coenobia achieves accuracies of 98.63% and 97.32% with support vector machine and artificial neural network, respectively. According to the results, it is possible to consider the proposed methodology as an alternative to the traditional technique for algae counting. In addition, the database used for the developing of the proposed methodology is publicly available.