Mariane dos Santos
Universidade Federal do Rio Grande do Sul
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Mariane dos Santos.
BMC Nephrology | 2013
Jonathan Fraportti do Nascimento; Luis Henrique Santos Canani; Fernando Gerchman; Patrícia Garcia Rodrigues; Gabriel Joelsons; Mariane dos Santos; Sane Vianna Pereira; Francisco José Veríssimo Veronese
BackgroundTo investigate gene expression of podocyte-specific proteins in urine of diabetes and prediabetes subjects and the association of these proteins with albuminuria.MethodsFifteen controls, 19 prediabetes, and 67 diabetes subjects were included. Messenger RNA of nephrin, podocin, podocalyxin, synaptopodin, TRPC6, alpha-actinin-4, and TGF-β1 were measured using RT-PCR. Podocyte marker expression was correlated with albuminuria, glycemic control, and renal function. The diagnostic performance of the genes used to detect increased albuminuria was assessed using ROC curves and Poisson regressions.ResultsPodocyte marker expression was significantly higher in diabetic subjects. Urinary nephrin was correlated with increasing levels of albuminuria; risk of albuminuria increased by 20% for every one-unit increase in the log10 of nephrin mRNA. Nephrinuria was found in 53%, 71%, and 90% of normo-, micro-, and macroalbuminuric diabetes subjects, respectively (p = 0.023). Urinary nephrin, podocalyxin, TRPC6, podocin, and alpha actinin-4 were correlated with glycemic control and albuminuria but not with renal function.ConclusionsDiabetes subjects had higher urinary mRNA levels of podocyte proteins than nondiabetic subjects, even the normoalbuminuric patients. Nephrinuria was correlated with diabetic nephrophathy stage and predicted pathological albuminuria. Urinary mRNA levels of podocyte markers of prediabetic subjects did not differ from controls.
Life Sciences | 2018
Mariane dos Santos; Gaia Favero; Francesca Bonomini; Alessandra Stacchiotti; Luigi F. Rodella; Francisco José Veríssimo Veronese; Rita Rezzani
Aims: Since lupus nephritis (LN) etiopathogenesis is not fully understood, herein we investigated the morphological basis of LN in mice induced with pristane. Main methods: To evaluate the melatonin effects in these animals, we studied the renal cytoarchitecture by means of morphological analyses, immunofluorescence expression of specific markers related to fibrosis, oxidative stress, inflammation and apoptosis. Key findings: We observed that pristane‐LN mice have serious alterations in the kidney cytoarchitecture, i.e. tubular degeneration, glomerular hypercellularity, matrix mesangial expansion and interstitial inflammation. The pristane‐induced LN mice treated with melatonin exhibited a well preserved cytoarchitecture. Significance: Our results document that LN etiopathogenesis is related to both tubular damage and glomerular lesions. We suggest that it is essential to take in consideration both these lesions for LN diagnosis and classification. Clearly, we show that the use of melatonin may be a possible therapeutic strategy for improvement the renal injury in this disorder. Graphical abstract: Figure. No Caption available.
Obesity | 2015
Sane Vianna Pereira; Mariane dos Santos; Patrícia Garcia Rodrigues; Jonathan Fraportti do Nascimento; João Rodolfo Teló Timm; Rafael Zancan; Rogério Friedman; Francisco José Veríssimo Veronese
The aim of this study was to correlate different degrees of excess weight with the expression of podocyte‐associated messenger RNAs (mRNAs) in urine.
Autoimmunity | 2018
Mariane dos Santos; Priscila Tamar Poletti; Gaia Favero; Alessandra Stacchiotti; Francesca Bonomini; Carolina Caruccio Montanari; Silvia Bona; Norma Anair Possa Marroni; Rita Rezzani; Francisco José Veríssimo Veronese
Abstract Introduction: Lupus nephritis (LN) is one of the most severe complications of systemic lupus erythematosus. As murine models of LN are valuable tools to better understand its pathophysiology and to search for new effective treatments, we investigated the effects of the bioflavonoid quercetin on pristane-induced LN mice through histomorphological analyses. Methods: Immunofluorescence and biochemical assays were used to evaluate the expression of markers of inflammation (interleukin-6, IL-6; tumour necrosis factor-α, TNF-α), oxidative stress (catalase, CAT; superoxide dismutase 1, SOD1; thiobarbituric acid reactive substances, TBARS), apoptosis (Bax), and fibrosis (transforming growth factor-β1, TGF-β1). Glomerular and tubular ultrastructure was analysed, and tissue messenger RNA of podocin, podoplanin and α3β1-integrin were quantified using the real-time polymerase chain reaction. Results: Pristane-induced LN mice showed severe kidney injury, characterized by increased proteinuria, glomerular mesangial expansion and inflammation, high expression of the pro-fibrotic, apoptotic and prooxidant markers and reduction of antioxidants. In the kidney ultrastructure, foot process (FP) effacement, apoptotic mesangial cells and abnormal mitochondria with disrupted cristae were observed, along with suppressed tissue mRNA of podocin, podoplanin and α3β1-integrin. Treatment with quercetin in the pristane-induced LN mice model was nephroprotective, decreasing proteinuria levels and significantly lowering tissue expression of IL-6, TNF-α, TGF-β1, Bax and TBARS. Simultaneously, quercetin significantly increased CAT and SOD1 expressions in these mice. In addition, it was observed improvement of the kidney ultrastructure, and tissue mRNA of podocin, but not podoplanin and α3β1-integrin, was restored to the levels found in the control mice. Conclusion: In conclusion, these findings provide experimental evidence of the renoprotective effects of quercetin in the pristane-induced LN mice model. We suggest that quercetin effectively ameliorates the kidney damage caused by pristane, a bioflavonoid to be further evaluated as a new therapeutic strategy in this disease.
Jornal Brasileiro De Nefrologia | 2016
João Rodolfo Teló Timm; Cristina Karohl; Mariane dos Santos; Maysa Lucena de Souza; Rafael Zancan; Rafael de Almeida; Francisco José Veríssimo Veronese
INTRODUCTION Vitamin D reduces albuminuria in patients with chronic kidney disease (CKD) but its effects on glomerular podocytes are not entirely understood. OBJECTIVE To evaluate if cholecalciferol supplementation reduces the levels of podocyte-associated urine mRNAs in patients with CKD. METHODS A total of 27 patients with stages 2 to 4 CKD and suboptimal serum vitamin D [25(OH)D] levels were treated with cholecalciferol for 6 months. Serum 25(OH)D level, estimated glomerular filtration rate (eGFR), proteinuria, and urine mRNA of nephrin, podocin, podocalyxin, transient receptor potential cation channel 6, vascular endothelial growth factor A, and transforming growth factor beta were assessed before and after intervention. RESULTS eGFR declined at an average rate of -4.71 mL/min/1.73 m2 (p = 0.010 vs. baseline), being 28 ± 16 mL/min/1.73 m2 at six months. No changes in proteinuria or mineral and bone metabolism parameters were observed after cholecalciferol supplementation. Urinary podocyte-associated mRNAs did not change significantly after treatment. However, patients who achieved 25(OH)D level > 20 ng/mL at six months showed a trend of reduction of urinary nephrin and podocin mRNA levels; in patients with 25(OH)D that remained < 20 ng/mL there was a significant increase in urinary podocalyxin, and a trend of higher expression of urinary nephrin and podocin mRNA. CONCLUSION Six months of cholecalciferol supplementation had no effect on urine podocyte-associated mRNA profile of patients with advanced CKD. The protective effect of vitamin D or its analogues on the glomerular podocyte should be investigated in early stages of CKD with a longer treatment period.
International Journal of Clinical and Experimental Pathology | 2015
Mariane dos Santos; Rafael Nazario Bringhenti; Patrícia Garcia Rodrigues; Jonathan Fraportti do Nascimento; Sane Vianna Pereira; Rafael Zancan; Odirlei André Monticielo; Andrese Aline Gasparin; Waldir Pedro de Castro; Francisco José Veríssimo Veronese
Seminars in Arthritis and Rheumatism | 2017
Mariane dos Santos; Priscila Poletti; Patrícia Milhoransa; Odirlei André Monticielo; Francisco José Veríssimo Veronese
Archive | 2017
Carolina Caruccio Montanari; Elisa Ruiz Fülber; Thiago Pereira Itaquy; Lorenzo Casagrande Reggiani; Emilia Christina Pallares; Charel Neves Matos; Patrícia Milhoransa; Mariane dos Santos; Manoel Roberto Maciel Trindade; Francisco José Veríssimo Veronese
Italian journal of anatomy and embryology | 2017
Gaia Favero; Mariane dos Santos; Claudio Lonati; Alessandra Stacchiotti; Rita Rezzani
Archive | 2016
Maysa Lucena de Souza; Mariane dos Santos; Priscila Poletti; João Rodolfo Teló Timm; Carolina Caruccio Montanari; Rômulo Marx; Camila Cony Rodrigues; Francisco José Veríssimo Veronese
Collaboration
Dive into the Mariane dos Santos's collaboration.
Francisco José Veríssimo Veronese
Universidade Federal do Rio Grande do Sul
View shared research outputsJonathan Fraportti do Nascimento
Universidade Federal do Rio Grande do Sul
View shared research outputs