Marie-Claire Castello
University of Western Australia
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Featured researches published by Marie-Claire Castello.
Crop & Pasture Science | 2014
Daniel Real; Chris Oldham; Matthew N. Nelson; Janine Croser; Marie-Claire Castello; Arūnas P. Verbyla; Aneeta Pradhan; A.J. Van Burgel; P. Méndez; Enrique Correal; Natasha L. Teakle; Clinton Revell; Mike Ewing
Abstract. Tedera (Bituminaria bituminosa C.H. Stirton var. albomarginata and var. crassiuscula) has been identified as one of the most productive and drought-tolerant species of herbaceous perennial legumes based on 6 years of field evaluation in Western Australia in areas with Mediterranean climate and annual rainfall ranging from 200 to 600 mm. Importantly, tedera demonstrated broad adaptation to diverse soils, and some accessions have shown moderate levels of tolerance to waterlogging and salinity. Tedera exhibits minimal leaf shedding during summer and autumn. Economic modelling strongly suggests that giving livestock access to green tedera in summer and autumn will dramatically increase farm profit by reducing supplementary feeding. The breeding program (2006–12) evaluated the available genetic diversity of tedera for its field performance in seven nurseries with 6498 spaced plants in total covering a wide variation in rainfall, soils and seasons. Best overall plants were selected using a multivariate selection index generated with best linear unbiased predictors (BLUPs) of dry matter cuts and leaf retention traits. The breeding program also evaluated tedera for grazing tolerance, grazing preference by livestock, waterlogging tolerance, seed production, cold tolerance, disease susceptibility and presence of secondary compounds. Tedera is a diploid, self-pollinated species. Therefore, 28 elite parents were hand-crossed in several combinations to combine outstanding attributes of parents; F1 hybrids were confirmed with the aid of highly polymorphic, simple sequence repeat markers. The F1s were progressed to F4s by single-seed descent breeding. Elite parent plants were selfed for two generations to be progressed in the breeding program without hybridisation. Over time, selections from the crossing and selfing program will deliver cultivars of three ideotypes: (i) drought-tolerant, (ii) cold- and drought-tolerant, (iii) waterlogging- and drought-tolerant.
Crop & Pasture Science | 2017
Maria Pazos-Navarro; Marie-Claire Castello; Richard G. Bennett; Phillip Nichols; Janine Croser
Abstract. Subterranean clover (Trifolium subterraneum L.) is widely grown for its forage and ability to fix atmospheric nitrogen. Development of new varieties is constrained by the slow turnover time of generations, with only one generation per year possible under field conditions. We present an in vitro-assisted single-seed descent (IVASSD) technique, which enabled turnover of 2.7–6.1 generations per year across a diverse range of 27 T. subterraneum cultivars encompassing subspecies subterraneum, yanninicum and brachycalycinum. The IVASSD protocol accelerated the generation cycle in two ways: (i) time to floral initiation was minimised by growth under controlled temperature and extended photoperiod; and (ii) the seed-filling period was truncated and embryo and seed-coat dormancy avoided by the in vitro germination of immature seed on B5 medium plus L6KK overlay (0.525 mg gibberellic acid and 1.5 mg indole-butyric acid L–1). For the first time, an IVASSD system was validated on a full-scale breeding population with the production of 175 F7 recombinant inbred lines from an F4 population in less than one year. All F7 plants obtained were morphologically normal and fertile.
Crop & Pasture Science | 2014
Maria Pazos-Navarro; Janine Croser; Marie-Claire Castello; Padmaja Ramankutty; K. Heel; Daniel Real; D. J. Walker; Enrique Correal; Mercedes Dabauza
Abstract. Bituminaria bituminosa (common name tedera) is a drought-tolerant perennial pasture species of agronomic and pharmaceutical interest for Mediterranean climates. Considering the importance of this legume, in vitro experiments were conducted to develop protocols for plant regeneration from embryogenic calli of leaves, petioles and anthers to efficiently exploit and maintain selected important clones from the tedera breeding program. The type of explant was a key factor in the frequency of embryogenesis and the number of embryos per callus. For plant regeneration from cultured anthers, appropriate anther physiological state (uninucleate stage of microsporogenesis), stress treatments (electroporation, 25 Ω, 25 µF, 1500 V) and culture conditions were determined. A robust flow-cytometry method was developed to analyse the ploidy status of callus, in vitro shoots and in vivo acclimatised plants derived from anther and leaf explants.
Theoretical and Applied Genetics | 2009
Matthew N. Nelson; Annaliese S. Mason; Marie-Claire Castello; Linda Thomson; Guijun Yan; Wallace Cowling
Theoretical and Applied Genetics | 2011
Annaliese S. Mason; Matthew N. Nelson; Marie-Claire Castello; Guijun Yan; Wallace Cowling
Grass and Forage Science | 2015
Marie-Claire Castello; Janine Croser; Monika Lulsdorf; Padmaja Ramankutty; Aneeta Pradhan; Matthew N. Nelson; Daniel Real
Grass and Forage Science | 2016
Marie-Claire Castello; Katia Stefanova; Phillip Nichols; B.J. Nutt; Clinton Revell; Janine Croser
Crop Science | 2014
Aneeta Pradhan; Naghmeh Besharat; Marie-Claire Castello; Janine Croser; Daniel Real; Matthew N. Nelson
Microspore culture from interspecific hybrids of Brassica napus and B. carinata produces fertile progeny | 2006
Matthew N. Nelson; Marie-Claire Castello; L. Thomson; Anouska Cousin; Guijun Yan; Wallace Cowling
Molecular characterisation of microspore-derived progeny from the interspecific F1 of Brassica napus x B. carinata | 2011
Matthew N. Nelson; Annaliese S. Mason; Marie-Claire Castello; Clare O'Lone; Guijun Yan; Sheng Chen