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Dive into the research topics where Marie-Pierre Fache is active.

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Featured researches published by Marie-Pierre Fache.


The EMBO Journal | 2001

Identification of an axonal determinant in the C‐terminus of the sodium channel Nav1.2

Juan José Garrido; Fanny Fernandes; Pierre Giraud; Isabelle Mouret; Eric Pasqualini; Marie-Pierre Fache; Florence Jullien; Bénédicte Dargent

To obtain a better understanding of how hippocampal neurons selectively target proteins to axons, we assessed whether any of the large cytoplasmic regions of neuronal sodium channel Nav1.2 contain sufficient information for axonal compartmentalization. We show that addition of the cytoplasmic C‐terminal region of Nav1.2 restricted the distribution of a dendritic–axonal reporter protein to axons. The analysis of mutants revealed that a critical segment of nine amino acids encompassing a di‐leucine‐based motif mediates axonal compartmentalization of chimera. In addition, the Nav1.2 C‐terminus is recognized by the clathrin endocytic pathway both in non‐neuronal cells and the somatodendritic domain of hippocampal neurons. The mutation of the di‐leucine motif located within the nine amino acid sequence to alanines resulted in the loss of chimera compartmentalization in axons and of internalization. These data suggest that selective elimination by endocytosis in dendrites may account for the compartmentalized distribution of some proteins in axons.


Journal of Cell Biology | 2008

Protein kinase CK2 contributes to the organization of sodium channels in axonal membranes by regulating their interactions with ankyrin G

Aline Brechet; Marie-Pierre Fache; Anna Brachet; Géraldine Ferracci; Agnès Baude; Marie Irondelle; Sandrine Pereira; Christophe Leterrier; Bénédicte Dargent

In neurons, generation and propagation of action potentials requires the precise accumulation of sodium channels at the axonal initial segment (AIS) and in the nodes of Ranvier through ankyrin G scaffolding. We found that the ankyrin-binding motif of Nav1.2 that determines channel concentration at the AIS depends on a glutamate residue (E1111), but also on several serine residues (S1112, S1124, and S1126). We showed that phosphorylation of these residues by protein kinase CK2 (CK2) regulates Nav channel interaction with ankyrins. Furthermore, we observed that CK2 is highly enriched at the AIS and the nodes of Ranvier in vivo. An ion channel chimera containing the Nav1.2 ankyrin-binding motif perturbed endogenous sodium channel accumulation at the AIS, whereas phosphorylation-deficient chimeras did not. Finally, inhibition of CK2 activity reduced sodium channel accumulation at the AIS of neurons. In conclusion, CK2 contributes to sodium channel organization by regulating their interaction with ankyrin G.


Journal of Cell Biology | 2004

Endocytotic elimination and domain-selective tethering constitute a potential mechanism of protein segregation at the axonal initial segment.

Marie-Pierre Fache; Anissa Moussif; Fanny Fernandes; Pierre Giraud; Juan José Garrido; Bénédicte Dargent

The axonal initial segment is a unique subdomain of the neuron that maintains cellular polarization and contributes to electrogenesis. To obtain new insights into the mechanisms that determine protein segregation in this subdomain, we analyzed the trafficking of a reporter protein containing the cytoplasmic II–III linker sequence involved in sodium channel targeting and clustering (Garrido, J.J., P. Giraud, E. Carlier, F. Fernandes, A. Moussif, M.P. Fache, D. Debanne, and B. Dargent. 2003. Science. 300:2091–2094). Here, we show that this reporter protein is preferentially inserted in the somatodendritic domain and is trapped at the axonal initial segment by tethering to the cytoskeleton, before its insertion in the axonal tips. The nontethered population in dendrites, soma, and the distal part of axons is subsequently eliminated by endocytosis. We provide evidence for the involvement of two independent determinants in the II–III linker of sodium channels. These findings indicate that endocytotic elimination and domain-selective tethering constitute a potential mechanism of protein segregation at the axonal initial segment of hippocampal neurons.


Proceedings of the National Academy of Sciences of the United States of America | 2011

End-binding proteins EB3 and EB1 link microtubules to ankyrin G in the axon initial segment

Christophe Leterrier; Helene Vacher; Marie-Pierre Fache; Stéphanie Angles d'Ortoli; Francis Castets; Amapola Autillo-Touati; Bénédicte Dargent

The axon initial segment (AIS) plays a key role in maintaining the molecular and functional polarity of the neuron. The relationship between the AIS architecture and the microtubules (MTs) supporting axonal transport is unknown. Here we provide evidence that the MT plus-end-binding (EB) proteins EB1 and EB3 have a role in the AIS in addition to their MT plus-end tracking protein behavior in other neuronal compartments. In mature neurons, EB3 is concentrated and stabilized in the AIS. We identified a direct interaction between EB3/EB1 and the AIS scaffold protein ankyrin G (ankG). In addition, EB3 and EB1 participate in AIS maintenance, and AIS disassembly through ankG knockdown leads to cell-wide up-regulation of EB3 and EB1 comets. Thus, EB3 and EB1 coordinate a molecular and functional interplay between ankG and the AIS MTs that supports the central role of ankG in the maintenance of neuronal polarity.


Journal of Cell Biology | 2010

Ankyrin G restricts ion channel diffusion at the axonal initial segment before the establishment of the diffusion barrier

Anna Brachet; Christophe Leterrier; Marie Irondelle; Marie-Pierre Fache; Victor Racine; Jean-Baptiste Sibarita; Daniel Choquet; Bénédicte Dargent

Ion channel immobilization by ankyrin G is regulated by casein kinase 2 in immature hippocampal neurons.


Neuroscience Letters | 2010

Voltage-gated sodium channel organization in neurons: Protein interactions and trafficking pathways

Christophe Leterrier; Anna Brachet; Marie-Pierre Fache; Bénédicte Dargent

In neurons, voltage-gated sodium (Nav) channels underlie the generation and propagation of the action potential. The proper targeting and concentration of Nav channels at the axon initial segment (AIS) and at the nodes of Ranvier are therefore vital for neuronal function. In AIS and nodes, Nav channels are part of specific supra-molecular complexes that include accessory proteins, adhesion proteins and cytoskeletal adaptors. Multiple approaches, from biochemical characterization of protein-protein interactions to functional studies using mutant mice, have addressed the mechanisms of Nav channel targeting to AIS and nodes. This review summarizes our current knowledge of both the intrinsic determinants and the role of partner proteins in Nav targeting. A few fundamental trafficking mechanisms, such as selective endocytosis and diffusion/retention, have been characterized. However, a lot of exciting questions are still open, such as the mechanism of differentiated Nav subtype localization and targeting, and the possible interplay between electrogenesis properties and Nav concentration at the AIS and the nodes.


Biology of the Cell | 2003

Dynamic compartmentalization of the voltage-gated sodium channels in axons.

Juan José Garrido; Fanny Fernandes; Anissa Moussif; Marie-Pierre Fache; Pierre Giraud; Bénédicte Dargent

One of the major physiological roles of the neuronal voltage‐gated sodium channel is to generate action potentials at the axon hillock/initial segment and to ensure propagation along myelinated or unmyelinated fibers to nerve terminal. These processes require a precise distribution of sodium channels accumulated at high density in discrete subdomains of the nerve membrane. In neurons, information relevant to ion channel trafficking and compartmentalization into sub‐domains of the plasma membrane is far from being elucidated. Besides, whereas information on dendritic targeting is beginning to emerge, less is known about the mechanisms leading to the polarized distribution of proteins in axon. To obtain a better understanding of how neurons selectively target sodium channels to discrete subdomains of the nerve, we addressed the question as to whether any of the large intracellular regions of Nav1.2 contain axonal sorting and/or clustering signals. We first obtained evidence showing that addition of the cytoplasmic carboxy‐terminal region of Nav1.2 restricted the distribution of a dendritic‐axonal reporter protein to axons of hippocampal neurons. The analysis of mutants revealed that a di‐leucine‐based motif mediates chimera compartmentalization in axons and its elimination in soma and dendrites by endocytosis. The analysis of the others generated chimeras showed that the determinant conferring sodium channel clustering at the axonal initial segment is contained within the cytoplasmic loop connecting domains II‐III of Nav1.2. Expression of a soluble Nav1.2 II‐III linker protein led to the disorganization of endogenous sodium channels. The motif was sufficient to redirect a somatodendritic potassium channel to the axonal initial segment, a process involving association with ankyrin G. Thus, it is conceivable that concerted action of the two determinants is required for sodium channel compartmentalization in axons.


Journal of Neuroendocrinology | 2001

Regulation of Central Corticosteroid Receptors Following Short-Term Activation of Serotonin Transmission by 5-Hydroxy- L-Tryptophan or Fluoxetine

Alexandra Sémont; Marie-Pierre Fache; F. Hery; M. Faudon; F. Youssouf; M. Héry

Alterations of the hypothalamic‐pituitary‐adrenal (HPA) axis function characterized by a decreased negative feedback capacity are often associated with affective disorders and are corrected by treatment with antidepressant drugs. To gain a better understanding of the effects of the antidepressant drug fluoxetine, a specific serotonin (5‐HT) reuptake inhibitor, on central corticosteroid receptors, the effects of short‐term activation of serotonin transmission on central corticosteroid receptor expression were analysed in adrenalectomized (ADX) rats either supplemented or not with corticosterone. Serotonin transmission was stimulated either by a single injection of the 5‐HT precursor, 5‐hydroxy‐ l‐tryptophan (5‐HTP), or by a 2‐day treatment with fluoxetine. In ADX rats, administration of 5‐HTP decreased hippocampal mineralocorticoid (MR) and glucocorticoid (GR) receptor numbers 24 h later, while their respective mRNAs were unchanged and these effects of 5‐HTP were mediated by 5‐HT2 receptors. In the hypothalamus, GR mRNAs and binding sites decreased 3 h and 24 h after 5‐HTP, respectively. By contrast, fluoxetine treatment increased hippocampal MR and GR mRNAs and MR binding sites while GR number remained unchanged. In ADX rats supplemented with corticosterone, 5‐HTP and fluoxetine treatment had the same effects on corticosteroid receptors compared to those observed in non supplemented ADX rats: 5‐HTP decreased hippocampal MR and GR and hypothalamic GR while fluoxetine treatment increased hippocampal MR. These results show that short‐term stimulation of 5‐HT transmission by 5‐HTP decreases hippocampal and hypothalamic corticosteroid receptor numbers through a corticosterone‐independent mechanism. It is hypothesized that the delayed maximal increase in extracellular 5‐HT contents after fluoxetine treatment, due to negative feedback regulations induced by the activation of 5‐HT1A and 5‐HT1B autoreceptors, is not the primary cause for the delayed normalization of corticosteroid receptor numbers that regulates the HPA axis functioning.


Neuroendocrinology | 1999

Effect of Serotonin Inhibition on Glucocorticoid and Mineralocorticoid Expression in Various Brain Structures

Alexandra Sémont; Marie-Pierre Fache; L. Ouafik; M. Héry; M. Faudon; F. Hery

Many studies have shown the existence of functional interactions between central neurotransmitter systems and the hypothalamo-pituitary adrenal axis. Mineralocorticoid receptors (MR) and glucocorticoid receptors (GR) are regulated by multiple factors including glucocorticoids themselves. Neurotransmitters such as serotonin (5-hydroxytryptamine: 5-HT) can regulate brain corticosteroid receptors in a complex way. The present study examined the short-term (48 h) effects of parachlorophenylalanine (PCPA), a drug which specifically inhibits 5-HT synthesis, on corticosteroid receptor levels and on the expression of their respective messenger ribonucleic acids (mRNA) in the rat hippocampus, hypothalamus and brain stem. The study was performed in bilaterally adrenalectomized animals, in order to avoid potential drug-induced changes in plasma corticosterone levels, which could secondarily regulate MR and GR. Short-term inhibition of 5-HT synthesis by PCPA significantly increased the number of hippocampal MR-binding sites. PCPA treatment did not alter the number of GR-binding sites in the hippocampus, hypothalamus and brain stem. We observed no change in the affinities of GR and MR sites in all the structures studied. In PCPA-treated rats, restoration of control 5-HT levels by injection of its immediate precursor, 5-hydroxytryptophan (5-HTP) brings the number of hippocampal MR-binding sites back to control levels. It can therefore be concluded that the increase in number of MR-binding sites induced by acute PCPA treatment is dependent on the decrease in 5-HT levels. The increase in hippocampal MR binding sites was correlated with an induction of their messengers, suggesting that 5-HT modulates the synthesis of MR protein. Although PCPA did not modify the number of hippocampal GR-binding sites, a decrease in hippocampal GR mRNA expression was observed. This study shows that 5-HT inhibits hippocampal mineralocorticoid receptor synthesis and that this effect is not mediated by changes in corticosterone hormone secretion, and illustrates the existence of complex mechanisms for corticosteroid receptor regulation in the hippocampus.


Journal of Neurochemistry | 1993

N-methyl-D-aspartic acid/glycine interactions on the control of 5-hydroxytryptamine release in raphe primary cultures.

D. Becquet; M. Héry; Paule Deprez; M. Faudon; Marie-Pierre Fache; P. Giraud; F. Hery

Abstract: Glutamic acid and glycine were quantified in cells and medium of cultured rostral rhombencephalic neurons derived from fetal rats. In the presence of 1 mM Mg2+, NMDA (50 μM) significantly stimulated (by 69%) release of newly synthesized 5‐[3H]hydroxytryptamine ([3H]5‐HT). d‐2‐Amino‐5‐phosphonopentanoate (AP‐5; 50 μM) blocked the stimulatory effect of NMDA. AP‐5 by itself inhibited [3H]5‐HT release (by 25%), suggesting a tonic control of 5‐HT by glutamate. In the absence of Mg2+, basal [3H]5‐HT release was 60% higher as compared with release with Mg2+. AP‐5 blocked the increased [3H]5‐HT release observed without Mg2+, suggesting that this effect was due to the stimulation of NMDA receptors by endogenous glutamate. Glycine (100 μM) inhibited [3H]5‐HT release in the absence of Mg2+. Strychnine (50 μM) blocked the inhibitory effect of glycine, indicating an action through strychnine‐sensitive inhibitory glycine receptors. The [3H]5‐HT release stimulated by NMDA was unaffected by glycine. In contrast, when tested in the presence of strychnine, glycine increased NMDA‐evoked [3H]5‐HT release (by 22%), and this effect was prevented by a selective antagonist of the NMDA‐associated glycine receptor, 7‐chlorokynurenate (100 μM). 7‐Chlorokynuren‐ate by itself induced a drastic decrease in [3H]5‐HT release, indicating that under basal conditions these sites were stimulated by endogenous glycine. These results indicate that NMDA stimulated [3H]5‐HT release in both the presence or absence of Mg2+. Use of selective antagonists allowed differentiation of a strychnine‐sensitive glycine response (inhibition of [3H]5‐HT release) from a 7‐chlorokynurenate‐sensitive response (potentiation of NMDA‐evoked [3H]5‐HT release).

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Juan José Garrido

Autonomous University of Madrid

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