Marina R. Cunha

Mud volcanism in the Gulf of Cadiz: results from the TTR-10 cruise

A new deep water mud volcano field (between 2000 and 3500 m water depth) was discovered in the deep South Portuguese margin, as well as several new mud volcanoes in the South Spanish and Western Moroccan margins of the Gulf of Cadiz, during the TTR-10 (Training Through Research, UNESCO/IOC) cruise, in July/August 2000. This work followed the discovery of a large mud volcano field in the Gulf of Cadiz, first investigated during the TTR-9 cruise [Gardner (2001) Geophys. Res. Lett. 28, 339–342; Kenyon et al. (2000) IOC, Technical series no. 56]. The discoveries were made based on a SEAMAP side-scan sonar mosaic and multibeam bathymetry (SEABEAM) collected in the area by the Naval Research Laboratory (NRL), Washington, DC, USA, in 1992, kindly released for this purpose. Single-channel seismics, long-range side-scan sonar (OKEAN), TV-controlled grab, hull-mounted 3.5-kHz profiler and coring were used to investigate several seafloor features observed on the side-scan sonar imagery, in the South Iberia, Spanish and Moroccan margins of the Gulf of Cadiz, which were confirmed to be mud volcanoes. The typical structures related to fluid venting in the Gulf of Cadiz are essentially represented by conical mud volcanoes with diameters ranging from several tens of meters to 4 km and heights that can reach 200 m. Some of these structures appear to be aligned along major conjugate NE–SW and NW–SE trending faults that can be identified on the side-scan sonar imagery. The new field discovered in the South Portuguese margin is the deepest in the Gulf of Cadiz area and includes three new mud volcanoes – Bonjardim, Olenin and Carlos Ribeiro – which seem to be quite active, with near-surface gas hydrate occurrence and a high saturation in H2S and hydrocarbon gases (mainly methane) in the mud breccia and overlying pelagic sediments. Gas hydrates were recovered from the Bonjardim mud volcano. An intensely gassified mud breccia, with one fragment of semi-consolidated claystone with a thin bituminous veneer at the surface, was recovered from the Carlos Ribeiro mud volcano. The fauna recovered consists mainly of pogonophoran worms belonging to several species and undetermined species of Foraminifera. Three new mud volcanoes were also discovered in the NW Moroccan margin: Rabat, Student and Jesus Baraza. These show a richer fauna that includes several species of molluscs, polychaetes, pogonophoran worms, crustaceans, echinoderms and some fragments of dead coral (Madrepora and Lophelia). Carbonate crusts were recovered from the Student mud volcano. The Ginsburg mud volcano, discovered during the previous TTR-9 cruise, was revisited and gas hydrates recovered once again. A new mud volcano was also discovered in the Spanish margin, Tasyo, where evidence was found of coral build-ups on the hard substratum of the mud volcanic edifice.

DNA barcoding reveals cryptic diversity in marine hydroids (Cnidaria, Hydrozoa) from coastal and deep-sea environments

Fifty‐six sequences of the mitochondrial 16S RNA gene were generated for hydroids, belonging to six nominal families — Eudendriidae, Lafoeidae, Haleciidae, Sertulariidae, Plumulariidae and Aglaopheniidae — collected from bathyal environments of the Gulf of Cadiz (22 haplotypes), Greenland (1 haplotype), Azores (1 haplotype), the shallow waters of the UK (17 haplotypes) and Portugal (2 haplotypes). When combined and analysed with 68 additional sequences published in GenBank, corresponding to 63 nominal species of these families (nine species in common between the GenBank sequences and those presented by the authors), cryptic species were detected (e.g. two species of Nemertesia and other of Lafoea), as well as apparent cases of conspecificity (e.g. Nemertesia antennina and N. perrieri and Aglaophenia octodonta, A. pluma and A. tubiformis). Other taxonomic inconsistencies were found in the data including cases where species from different genera clustered together (e.g. Sertularia cupressina, Thuiaria thuja, Abietinaria abietina and Ab. filicula). The mitochondrial 16S rRNA proved to be a useful DNA ‘barcode’ gene for hydroids, not only allowing discrimination of species, but also in some cases of populations, genera and families, and their intra‐ or interphylogenetic associations. Although still under‐represented in public data bases, the 16S rRNA gene is starting to be used frequently in the study of hydroids. These data provide powerful complementary evidence for advancing our understanding of hydrozoan systematics.

Environmental gradients in a southern Europe estuarine system: Ria de Aveiro, Portugal implications for soft bottom macrofauna colonization

Four seasonal sampling surveys were carried out between December 1985 and September 1986 in Canal de Mira (Ria de Aveiro, Portugal). A total of 40 sampling stations, distributed over 13 transects, was used. Salinity, temperature, dissolved oxygen and pH of the water mass were measured. Sediment temperature, and salinity and pH of interstitial water were determined. Sediment variables also included granulometric composition and organic matter contents. Bottom macrofauna samples were collected at each station.Ordination (PCA and MDS) and classification of the sampling stations were performed, using the physicochemical and the biological data sets separately. Average linkage cluster analysis using the unweighted paired-group method, arithmetic averages, was used for both sets of data.With a salinity range from 35.1‰ to 0.0‰, Canal de Mira behaves like a tidally and seasonally poikilohaline estuary. Water temperature (8.5–24.7°C) decreased along the channel towards its inner part during the cold season; an inverse and more pronounced trend was observed during the hot season. Dissolved oxygen contents was generally high during the day (50% to 240% saturation). Oversaturation was observed throughout the growing season, with peaks in areas with large amounts of rooted vegetation. The pH values, largely correlated with dissolved oxygen, ranged from 6.8 to 8.9. Four types of sediment were present in Canal de Mira, medium and muddy sands being dominant.Two major gradients were identified: (i) a typical longitudinal estuarine gradient, associated with distance from the mouth, representing physicochemical variables such as tidal amplitude, salinity and temperature; this gradient was accompanied by an upstream increase in dominance; the community composition changes were mainly related to salinity; (ii) a lateral gradient, related to current velocity, depth and sediment composition; the subtidal community had a comparatively low species richness and abundance. Groups of stations could be recognized along the environmental gradients. Benthic community changes, however, appeared to be gradual rather than marked by abrupt transitions.

Systematic and Evolutionary Insights Derived from mtDNA COI Barcode Diversity in the Decapoda (Crustacea: Malacostraca)

Background Decapods are the most recognizable of all crustaceans and comprise a dominant group of benthic invertebrates of the continental shelf and slope, including many species of economic importance. Of the 17635 morphologically described Decapoda species, only 5.4% are represented by COI barcode region sequences. It therefore remains a challenge to compile regional databases that identify and analyse the extent and patterns of decapod diversity throughout the world. Methodology/Principal Findings We contributed 101 decapod species from the North East Atlantic, the Gulf of Cadiz and the Mediterranean Sea, of which 81 species represent novel COI records. Within the newly-generated dataset, 3.6% of the species barcodes conflicted with the assigned morphological taxonomic identification, highlighting both the apparent taxonomic ambiguity among certain groups, and the need for an accelerated and independent taxonomic approach. Using the combined COI barcode projects from the Barcode of Life Database, we provide the most comprehensive COI data set so far examined for the Order (1572 sequences of 528 species, 213 genera, and 67 families). Patterns within families show a general predicted molecular hierarchy, but the scale of divergence at each taxonomic level appears to vary extensively between families. The range values of mean K2P distance observed were: within species 0.285% to 1.375%, within genus 6.376% to 20.924% and within family 11.392% to 25.617%. Nucleotide composition varied greatly across decapods, ranging from 30.8 % to 49.4 % GC content. Conclusions/Significance Decapod biological diversity was quantified by identifying putative cryptic species allowing a rapid assessment of taxon diversity in groups that have until now received limited morphological and systematic examination. We highlight taxonomic groups or species with unusual nucleotide composition or evolutionary rates. Such data are relevant to strategies for conservation of existing decapod biodiversity, as well as elucidating the mechanisms and constraints shaping the patterns observed.

Europe’s Grand Canyon: Nazaré submarine canyon

The Nazare submarine canyon extends similar to 210 km westward from the coast of Portugal, down to a water depth of > 4300 m. The considerable habitat heterogeneity found throughout the canyon is affected by strong currents and high turbidity, especially in the upper parts of the canyon. The canyon morphology comprises steep slopes, scarps, terraces, and overhangs, and a deeply incised thalweg is found in the lower part of the canyon. The seabed within the canyon is composed of varying proportions of rock and sediments that range from sand to fine mud. This great variation in physical environment is reflected by the varied fauna inhabiting the canyon. Diversity tends to decrease with depth, but there is also continual replacement of species with increasing water depth. Certain groups, such as the gorgonians and sea lilies, tend to be found on rocky surfaces, while large protozoans dominate the sediments at 3400-m depth. In addition to describing the fauna of Nazare Canyon, we discuss experiments undertaken as part of the HERMES project to elucidate the ecosystem function processes operating in the deeper parts of the canyon.

Hydrothermal Vents and Methane Seeps: Rethinking the Sphere of Influence

Although initially viewed as oases within a barren deep ocean, hydrothermal vent and methane seep communities are now recognized to interact with surrounding ecosystems on the sea floor and in the water column, and to affect global geochemical cycles. The importance of understanding these interactions is growing as the potential rises for disturbance from oil and gas extraction, seabed mining and bottom trawling. Here we synthesize current knowledge of the nature, extent and time and space scales of vent and seep interactions with background systems. We document an expanded footprint beyond the site of local venting or seepage with respect to elemental cycling and energy flux, habitat use, trophic interactions, and connectivity. Heat and energy are released, global biogeochemical and elemental cycles are modified, and particulates are transported widely in plumes. Hard and biotic substrates produced at vents and seeps are used by “benthic background” fauna for attachment substrata, shelter, and access to food via grazing or through position in the current, while particulates and fluid fluxes modify planktonic microbial communities. Chemosynthetic production provides nutrition to a host of benthic and planktonic heterotrophic background species through multiple horizontal and vertical transfer pathways assisted by flow, gamete release, animal movements, and succession, but these pathways remain poorly known. Shared species, genera and families indicate that ecological and evolutionary connectivity exists among vents, seeps, organic falls and background communities in the deep sea; the genetic linkages with inactive vents and seeps and background assemblages however, are practically unstudied. The waning of venting or seepage activity generates major transitions in space and time that create links to surrounding ecosystems, often with identifiable ecotones or successional stages. The nature of all these interactions is dependent on water depth, as well as regional oceanography and biodiversity. Many ecosystem services are associated with the interactions and transitions between chemosynthetic and background ecosystems, for example carbon cycling and sequestration, fisheries production, and a host of non-market and cultural services. The quantification of the sphere of influence of vents and seeps could be beneficial to better management of deep-sea environments in the face of growing industrialization.

The use of the DNA barcode gene 16S mRNA for the clarification of taxonomic problems within the family Sertulariidae (Cnidaria, Hydrozoa)

Moura, C. J., Cunha, M. R., Porteiro, F. M. & Rogers, A. D. (2011). The use of the DNA barcode gene 16S mRNA for the clarification of taxonomic problems within the family Sertulariidae (Cnidaria, Hydrozoa). —Zoologica Scripta, 40, 520–537.

NEW RECORD OF ''BATHYMODIOLUS'' MAURITANICUS COSEL 2002 FROM THE GULF OF CADIZ (NE ATLANTIC) MUD VOLCANOES

Abstract The “Bathymodiolus” childressi group is the most geographically diverse assemblage of deep-sea mussel species. In this paper we consider several possible hypotheses to explain the present biogeographic distribution of the “B.” childressi species complex. Mussels were collected for the first time from mud volcanoes in the Gulf of Cadiz (NE Atlantic Ocean) during the training through research (TTR) 16 research expedition in 2006. Preliminary observations of the shell features indicate that they belong to the “B.” childressi species complex, which has been recognized as morphologically and genetically distinct from other Bathymodiolus species. Molecular analyses of two mitochondrial genes (COI-5 and ND4) were used to characterize the new mussel population from the Gulf of Cadiz (GOC) and to determine their phylogenetic relationships with other members of the “B.” childressi group. The results indicate that the GOC mussels are conspecific with “Bathymodiolus” mauritanicus Cosel (2002), described from West Africa margin, and support a previous hypothesis that “B.” mauritanicus is an amphi-Atlantic species

Chemosymbiotic bivalves from the mud volcanoes of the Gulf of Cadiz, NE Atlantic, with descriptions of new species of Solemyidae, Lucinidae and Vesicomyidae

Abstract The chemosymbiotic bivalves collected from the mud volcanoes of the Gulf of Cadiz are reviewed. Of the thirteen species closely associated with chemosynthetic settings two Solemyidae, Solemya (Petrasma) elarraichensis sp. n. and Acharax gadirae sp. n., one Lucinidae, Lucinoma asapheus sp. n., and one Vesicomyidae, Isorropodon megadesmus sp. n. are described and compared to close relatives of their respective families. The biodiversity and distribution of the chemosymbiotic bivalves in the Gulf of Cadiz are discussed and compared to the available information from other cold seeps in the Eastern Atlantic and Mediterranean. Although there is considerable similarity at the genus level between seep/mud volcano fields in the Eastern Atlantic and Mediterranean, there is little overlap at the species level. This indicates a high degree of endemism within chemosymbiotic bivalve assemblages.

Life history, reproduction, and production of Gammarus chevreuxi (Amphipoda:Gammaridae) in the Ria de Aveiro, northwestern Portugal

Abstract We quantified abundance and biomass of the amphipod Gammarus chevreuxi Sexton from benthic cores, sampled monthly over 1 y in the upper reaches of Canal de Mira, the southern arm of the Ria de Aveiro, Portugal. Abundance and biomass showed no clear seasonal patterns but were associated with variation in salinity, dissolved oxygen, and chlorophyll a concentration. Maximum abundance and biomass occurred at relatively low temperature and high food availability, conditions that likely increased survival and/or reproduction. The population showed a semiannual, iteroparous life cycle. Mean life span was ∼6 mo, with overwintering individuals hatched in autumn showing higher longevity than individuals hatched in spring. Breeding was continuous, although juvenile recruitment peaked in early autumn, winter, and early spring. Sudden fluctuations in abundance and size structure of the population may have resulted from immigration during autumn and emigration during winter. Mean fecundity (8 embryos/brood) and intramarsupial loss (0–18%) were low compared with studies of other Gammarus species. Variation in fecundity and intramarsupial loss was mostly explained by size of incubating females. The volume of the embryos was highly variable but not significantly correlated with female size or other reproductive traits. The Hynes and the Morin– Bourassa methods yielded similar estimates of annual production (∼46 g AFDM m−2 y−1) and P/B̄ ratio (11/y).