Mario Ruiz-López

Extracts from green and brown seaweeds protect tomato (Solanum lycopersicum) against the necrotrophic fungus Alternaria solani

Several reports have shown that crude or purified extracts of green, brown, and red seaweeds induce protection against fungal, bacterial, and viral pathogens in plants. In this work, we report that polysaccharide-enriched seaweed extracts obtained from green, Ulva lactuca and Caulerpa sertularioides, and brown algae, Padina gymnospora and Sargassum liebmannii, induced protection against the necrotrophic fungus Alternaria solani in tomato plants (Solanum lycopersicum). Protein activity of defense-related proteins polyphenol oxidase, guaiacol peroxidase and proteinase inhibitors together with expression levels of systemic wound response (SWRP) genes were also measured in leaf samples after algal extract treatment. All extracts were shown to reduce necrotic lesions induced by A. solani, particularly those obtained from U. lactuca and P. gymnospora. U. lactuca extracts induced the expression of SWRP genes, including defense, signal pathway, and protease genes, whereas those obtained from C. sertularioides, P. gymnospora and S. liebmannii showed almost no induction of SWRP genes, suggesting that extracts from the latter, whose carbohydrate composition varied from that of U. lactuca, may act through mechanisms other than the jasmonic acid/systemin wound-response pathway.

Effect of a bacterial inoculum and additive on dry matter in situ degradability of sugarcane silage

Abstract The objective of this study was to evaluate the effect of adding a bacterial inoculum and a handmade additive to sugarcane silage (SCS) on the in situ digestibility of dry matter (DM). The treatments were: T1) sugarcane silage (SCS) and T2) sugarcane silage with 1% inoculum and 1% additive (SCS+). The bacterial inoculum consisted of 10.0% molasses, 1.0% yogurt, 5.0% chicken manure, 0.5% urea, and 83.0% water, and the additive was formulated with 1.0% urea, 0.1% ammonium sulfate, and 0.25% phosphorus. In situ dry matter digestibility (DMD) was determined using the nylon bag technique with four cows equipped with ruminal fistulas. Cows were fed with ensiled sugarcane supplemented with 1 kg of commercial concentrate. 5 g of ground sample for each sugarcane treatment were weighted in nylon bags and incubated for 4, 8, 12, 24, 48, and 72 h in a completely randomized design with six replicates. The DMD (%) was higher ( P

Iron bioavailability of Lupinus rotundiflorus seeds and roots in low-iron-diet treated rats

1 Universidad de Guadalajara, Centro Universitario del Sur, Centro de Investigación en Comportamiento Alimentario y Nutrición. Guadalajara, Jalisco, México. 2 Universidad de Guadalajara, Centro Universitario de Ciencias Biológicas y Agropecuarias, Departamento de Botánica y Zoología. Camino Ing. Ramón Padilla, 2100, Predio Las Agujas, Nextipac, Zapopan, Jalisco, México. Correspondência para/ Correspondence to: MA RUIZ-LOPEZ. E-mail: .

Efecto del tratamiento térmico sobre la composición química y minerales en semillas de lupinos silvestres

Wild lupine consumption has restrictions due to the presence of alkaloids; however, these components can be reduced with a suitable thermal treatment. The ai...