Marion Sweeney
University of Wales
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Publication
Featured researches published by Marion Sweeney.
British Journal of Haematology | 2005
Kenneth Ian Mills; Amanda F. Gilkes; Val Walsh; Marion Sweeney; Rosemary E. Gale
Mutations of the FLT3 gene, a receptor tyrosine kinase, are the most frequent genetic alteration reported in acute myeloid leukaemia, with internal tandem duplications (ITD) or mutations within the activating loop (AL) reported at a frequency of around 24% and 6%, respectively. ITD mutations have associated with a poor prognosis. In this study we have used polymerase chain reaction (PCR), combined with restriction enzyme digestion for the detection of AL mutations, with the DNA products separated on the Agilent 2100 Bioanalyser using a DNA‐500 kit. This analysis enabled the rapid identification of mutations in FLT3, approximate sizing of the ITD, an estimate of the proportion of mutant RNA and in some cases, specific heteroduplex patterns associated with triplet deletions. Our data shows that approximately 16% of the patients examined had an ITD mutation and over 13% had a mutation in the AL including triplet deletions involving codons 835/836 and point mutations in codon D839. Based on the sensitivity and speed of the bioanalyser, we suggest that this method is invaluable and provides an improvement to the current use of agarose gels for the analysis of FLT3 PCR products.
British Journal of Haematology | 2000
Kenneth Ian Mills; V. Walsh; Amanda F. Gilkes; Marion Sweeney; T. Mirza; L. J. Woodgate; Geoffrey Brown; Alan Kenneth Burnett
Retinoic acid has the ability to induce differentiation in some myeloid leukaemia cell lines and has been used to induce remission in acute promyelocytic leukaemia patients. We have analysed changes in gene expression, by differential display, in HL60 cells exposed to all‐trans retinoic acid (ATRA) for only 1 h. Only about 0.4% of the genes examined by this technique showed changes in expression level, and all four of the gene fragments identified were downregulated during the short 1 h exposure. Two of the fragments were novel, a third was MYC and the fourth was the FUS proto‐oncogene. Northern analysis showed that FUS was downregulated within 1 h only during induced neutrophil differentiation but not at all during induced monocyte differentiation. Unlike the sensitive cell lines, ATRA‐resistant cell lines did not show a downregulation of FUS over a 24 h period of exposure to ATRA. Using a semiquantitative PCR analysis, no difference in FUS levels was observed between ATRA‐sensitive and ‐resistant cell lines. A similar analysis was carried out on primary acute myeloid leukaemia (AML), peripheral stem cell harvests (PBSC) and cord blood samples. The PBSC and cord blood samples had FUS levels that were similar or generally less than the cell lines. However, much higher levels were seen in 63% of the AML samples examined. The data presented are consistent with previous reports for a role for FUS in the promotion and maintenance of cellular proliferation.
Blood | 2002
Richard Lawrence Darley; Lorna Pearn; Nader Omidvar; Marion Sweeney; Janet Fisher; Sarah Phillips; Terence George Hoy; Alan Kenneth Burnett
FEBS Letters | 1998
Kenneth Ian Mills; Amanda F. Gilkes; Marion Sweeney; M. A. Choudhry; L. J. Woodgate; Christopher M. Bunce; Geoffrey Brown; Alan Kenneth Burnett
Cytometry | 1993
A.Denise White; Marion Sweeney
Archive | 2013
Alan Kenneth Burnett; Richard Lawrence Darley; Lorna Pearn; Nader Omidvar; Marion Sweeney; Janet Fisher; Sarah Phillips
Archive | 2013
Richard Lawrence Darley; Lorna Pearn; Nader Omidvar; Marion Sweeney; Janet Fisher; Sarah Phillips; Terry Hoy; Alan Kenneth Burnett
Archive | 2002
Richard Lawrence Darley; Lorna Pearn; Nader Omidvar; Marion Sweeney; Janet Fisher; Sarah Phillips; Terry Hoy; Alan Kenneth Burnett
Biochemical Society Transactions | 2000
Kenneth Ian Mills; Marion Sweeney; S. J. Austin; V. Walsh; E. J. Dann; Alan Kenneth Burnett
Archive | 1999
L. J. Woodgate; E. J. Walker; Amanda F. Gilkes; V. Walsh; Marion Sweeney; Ken I. Mills; Alan Kenneth Burnett