Marit H. Lie

The importance of host tree age, size and growth rate as determinants of epiphytic lichen diversity in boreal spruce forests

The amount of large and old trees has decreased in the boreal forests during the last centuries of forestry. Such trees are important habitats for epiphytic lichens and there is a growing concern for lichen species that are associated with large and old trees. However, only little is known about the relative importance of tree size versus age as determinants of lichen diversity. Here we have determined the size, age and growth rate of 157 Norway spruce trees and recorded the occurrence of epiphytic lichen species on their branches and lower stems. The study includes crustose lichens and was done in two old-growth forests in SE Norway. Tree age and tree size were the parameters that explained the largest part of epiphytic lichen diversity. Only the growth rate of the most recent time period, i.e. 1984–2004, showed a statistically significant relationship to diversity. There was no indication of a stabilising species number with increasing tree age. Slow-growing and old trees were, however, mainly of importance to the lichen species growing on stems, and this set of species were in general adversely affected by a large amount of branches. The opposite was the case for the species that were confined to branches as their diversity increased when the amount of branches increased. Our study adds empirical data to support the importance of large and old trees as bearers of biodiversity in boreal forests. Site preservation and patch retention of groups of old and large trees is recommended as measures to maintain epiphytic lichen diversity.

Epiphytic lichen biomass in a boreal Norway spruce forest

Alectorioid and foliose lichens were weighed from full-size branches of Picea abies cut at two canopy heights in 100 trees in an old subalpine forested area in eastern Norway. The mean lichen biomass per branch decreased from 46 to 37 g moving upwards from 2–3 to 5–6 m canopy height. The lichen biomass correlated strongly with branch size variables, branch diameter alone explaining 48 of the variation in lichen biomass per branch ( n =200). The alectorioid/foliose biomass ratio increased from 0·149 at 2–3 m to 0·316 at 5–6 m. Site factors reflecting openness of the canopy were computed for the branch at 2–3 m in all trees. The alectorioid/foliose biomass ratio increased significantly with the indirect site factors, suggesting that light, or factors associated with light, determine the balance between these two growth forms in tree canopies. Within the alectorioid biomass component, genera with usnic acid ( Alectoria , Usnea ) decreased with height, whereas the genus Bryoria with melanic pigments increased. According to the literature, dark melanic pigments have higher visible light screening efficiency than the yellowish usnic acid. Such patterns suggest a functional role for cortical pigments in the niche differentiation of alectorioid lichens.

Molecular characterization of airborne fungal spores in boreal forests of contrasting human disturbance

In this study we present a new approach to characterize fungal diversity with DNA sequencing of mycelium grown from trapped airborne spores. Fungal spores were extracted systematically from air in three boreal forest sites (clear-cut, young and old-growth forests) using an air sampling device. Internal transcribed spacer (ITS) sequences from the nuclear ribosomal DNA (nrDNA) were generated, and the sequences most likely taxon affinities were established through DNA homology searches. Phylogenetic analyses were used to classify similar sequences into operational taxonomic units (OTUs). The analyses indicated that a total of 84 different OTUs had been sampled, 24 basidiomycetes and 60 ascomycetes. OTUs belonging to the ascomycete orders Helotiales and Pleosporales were most frequent (31 and 18 respectively). A total of 54, 29 and 33 OTUs were sampled, respectively, in the old-growth, young and clear-cut forest sites. Although heavy generalization should be avoided due to few replicates, the results could indicate that old-growth boreal forests have significantly higher airborne fungal species richness than recently managed forests. The study shows that the spore-trapping approach has a great potential for targeting and studying anonymous fungi.

A refined view on the "Green lie'' : Forest structure and composition succeeding early twentieth century selective logging in SE Norway

Abstract Logging exceeded growth and timber trees were sparse in Norwegian forests in the early 1900s. Still, the forest canopy was lush green and characterised by large tree-crowns. This situation was referred to as the “Green lie” and was advocated by foresters throughout Scandinavia as an argument in favour of forestry practices based on clear-felling. Here we examine effects of past selective loggings on forest structure and composition in a spruce forest landscape using dendroecology and historical records. Our results show that forests that were selectively logged up to the early 1900s could be structurally heterogeneous with multi-layered canopies, varying degree of openness and continuous presence of old trees across different spatial scales. Because the past forests were not clear-felled, a diverse forest structure in terms of tree species composition and age and diameter distribution was maintained over time, which could enable forest-dwelling species to persist during the early phase following the loggings in the past. This is in sharp contrast to the situation in most modern managed forest landscapes in Scandinavia. A better understanding of the link between loggings in the past- and present-day forest structure and diversity will contribute to rewarding discussions on forestry methods for the future.

Late-Holocene fire history as revealed by size, age and composition of the soil charcoal pool in neighbouring beech and spruce forest landscapes in SE Norway

European beech and Norway spruce are late successional tree species that have become rapidly dominant in northern Europe in late-Holocene. The northern distribution limit for natural beech forests is in SE Norway, where beech forests and boreal spruce forests meet. Here we have estimated the size, composition and age of the macroscopic charcoal pool to infer past fire history and the establishment of neighbouring Norwegian beech- and spruce forests. To encompass landscape level scales of variations in the charcoal pool, we have analysed the charcoal record in 100 soil cores that were collected using a restricted random procedure. The sizes of the soil charcoal pools ranged from 2 to 1214 g m−2, and they were significantly more spatially variable in the beech forest landscape than in the spruce forest landscape. We show that today’s beech forests took over the dominance from Norway spruce in the landscape about 300 years ago, and that fire disturbances on the landscape level preceded the establishment of beech in the former spruce forest landscape. Interestingly, large-scale fire disturbances have not occurred ever since beech gained dominance. Conversely, we show that today’s spruce forests took over the dominance from Scots pine in the landscape, and that also the establishment of spruce was preceded by fire disturbances on the landscape level.

Litter impair spruce seedling emergence in beech forests: a litter manipulation experiment

ABSTRACT European beech Fagus sylvatica and Norway spruce Picea abies are economically and ecologically important forest trees in large parts of Europe. Today, the beech forest reaches its northern distribution limit in south-eastern Norway and it is expected to expand northwards due to climate warming. This expansion will likely result in fundamental ecosystem changes. To increase our knowledge about the competitive balance between spruce and beech, we have investigated how beech and spruce litter affect spruce seedling emergence, growth and uptake of C and N. We did this in a seed-sowing experiment that included litter layer removal as well as reciprocal transplantations of litter layers between spruce and beech forests. Our results show that spruce seedling emergence was significantly impaired by both litter layer types, and especially so by the beech litter layer in the beech forest. The low seedling emergence in beech forests is concurrent with their lower light availability.

Fertilization changes chemical defense in needles of mature Norway spruce (Picea abies)

Nitrogen availability limits growth in most boreal forests. However, parts of the boreal zone receive significant levels of nitrogen deposition. At the same time, forests are fertilized to increase volume growth and carbon sequestration. No matter the source, increasing nitrogen in the boreal forest ecosystem will influence the resource situation for its primary producers, the plants, with possible implications for their defensive chemistry. In general, fertilization reduces phenolic compound concentrations in trees, but existing evidence mainly comes from studies on young plants. Given the role of the phenolic compounds in protection against herbivores and other forest pests, it is important to know if phenolics are reduced with fertilization also in mature trees. The evergreen Norway spruce is long-lived, and it is reasonable that defensive strategies could change from the juvenile to the reproductive and mature phases. In addition, as the needles are kept for several years, defense could also change with needle age. We sampled current and previous year needles from an N fertilization experiment in a Norway spruce forest landscape in south-central Norway to which N had been added annually for 13 years. We analyzed total nitrogen (N) and carbon (C), as well as low-molecular phenolics and condensed tannins. Needles from fertilized trees had higher N than those from controls plots, and fertilization decreased concentrations of many flavonoids, as well as condensed tannins in current year needles. In previous year needles, some stilbenes and condensed tannins were higher in fertilized trees. In control trees, the total phenolic concentration was almost five times as high in previous year needles compared with those from the current year, and there were great compositional differences. Previous year needles contained highest concentrations of acetophenone and stilbenes, while in the current year needles the flavonoids, and especially coumaroyl-astragalins dominated. Condensed tannins did not differ between current and previous year needles from control trees. In conclusion, the phenolic defense of current year needles of mature P.abies trees was strongly changed upon fertilization. This may imply that nitrogen deposition and forest fertilization leave forests less robust in a time when pests may take advantages of a changing climate.

FFL6_S1_L001_R1_001.fastq.gz part 2

Second part of the bzip-compressed fastq-file containing raw sequences (forward reads) from Illumina HiSeq of pooled fungal DNA. Split with HJSplit as recommended by Dryad (http://wiki.datadryad.org/Large_File_Transfer).