Mark Alejandro Quesada
Corning Inc.
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Publication
Featured researches published by Mark Alejandro Quesada.
Lab on a Chip | 2005
Po Ki Yuen; Norman H. Fontaine; Mark Alejandro Quesada; Prantik Mazumder; Richard Bergman; Eric J. Mozdy
In order to allow the design of increasingly sensitive label-free biosensors, compensation of environmental fluctuations is emerging as the dominant hurdle. The system and technique presented here utilize a unique combination of microfluidics, optical instrumentation, and image processing to provide a reference signal for each label-free biomolecular binding assay. Moreover, this reference signal is generated from the same sensor used to detect the biomolecular binding events. In this manner, the reference signal and the binding signal share nearly all common-mode noise sources (temperature, pressure, vibration, etc.) and their subtraction leaves the purest binding signal possible. Computational fluid dynamic simulations have been used to validate the flow behavior and thermal characteristics of the fluids inside the sensing region. This system has been demonstrated in simple bulk refractive index tests, as well as small molecule (biotin/streptavidin) binding experiments. The ability to perform not only simple binding but also control experiments has been discussed, indicating the wide applicability of the technique.
Electrophoresis | 2000
Dehai Liang; Liguo Song; Mark Alejandro Quesada; Zhaowu Tian; F.William Studier; Benjamin Chu
A new method to introduce the concentration gradient into the capillary has been developed and its application to DNA capillary electrophoresis is presented. The concentration gradient produced by mixing 5% w/v polyacrylamide‐co‐poly(N‐dimethylacrylamide) (PAM‐co‐PDMA) solution and 1 × Tris/N‐tris(hydroxymethyl)methyl‐3‐amino‐propanesulfonic acid/EDTA (TT) + 5 M urea buffer was successfully achieved by using two programmable syringe pumps with strict control of dead volume, flow rate, and pressure balance. This method has the advantages of high stability, reproducibility, and versatility. The column with concentration gradient greatly improved the resolution, especially for the large DNA fragments, due to a decrease in band width broadening with time. A column containing 2—4% w/v gradient in four steps had a longer read length, shorter separation time and better resolution (after 380 base) than that of 4% w/v single concentration polymer solution. The number of steps in the gradient had almost no effect on the performance. The change in the average concentration by relocating the position of the same step gradient, i.e., a combination of different low concentration to high concentration polymer solution ratios, resulted in a different migration time, read length and resolution.
Journal of the American Chemical Society | 2002
Anthony G. Frutos; Santona Pal; Mark Alejandro Quesada; Joydeep Lahiri
Archive | 2010
Adra Smith Baca; Karl W. Koch; Shari Elizabeth Koval; Prantik Mazumder; Mark Alejandro Quesada; Wageesha Senaratne; Todd Parrish St. Clair
Archive | 2007
Bruce Gardiner Aitken; Chong Pyung An; Mark Alejandro Quesada
Archive | 2014
Bruce Gardiner Aitken; Mark Alan Lewis; Mark Alejandro Quesada
Archive | 2007
Bruce Gardiner Aitken; Dana Craig Bookbinder; Sean M. Garner; Mark Alejandro Quesada
Archive | 2001
Anthony G. Frutos; Joydeep Lahiri; Santona Pal; Mark Alejandro Quesada
Archive | 2010
Chong Pyung An; Mark Alejandro Quesada
Archive | 2008
Bruce Gardiner Aitken; Chong Pyung An; Mark Alejandro Quesada