Mark D. Lim

The Preparation of Anaerobic Nitric Oxide Solutions for the Study of Heme Model Systems in Aqueous and Nonaqueous Media: Some Consequences of NOx Impurities

The reactions of nitric oxide (NO) have been a subject of broad interest among biochemists and chemists. This report discusses several quantitative techniques to handle and use NO gas that has been delivered from compressed cylinders. The focus is on techniques that minimize and avoid the presence of oxygen impurities, which when present, result in the generation of other nitrogen oxides (NO x). These NO x species typically exhibit different reactivity, and unless removed or quantified, their presence will complicate studies focusing on the reactions caused by NO itself.

Photochemical cleavage of nitrate ion coordinated to a Cr(III) porphyrin

Photolysis of the nitrato chromium(III) tetraphenylporphyrinato compound Cr(TPP)(NO3) (TPP, tetraphenylporphyrin) in toluene solution clearly leads to the formation of the Cr(IV) oxo complex Cr(TPP)(O) with a quantum yield of 0.011. The other product, NO2, was detected quantitatively by its reaction with the spin trapping agent 2,2,6,6-tetramethyl-piperidine-1-oxyl.

Chapter 3 – NO and NOx Interactions with Hemes

In this chapter, we will discuss the mechanistic studies of NO and NO x ( NO 2 − , ⋅ NO 2 , N 2 O 3 , etc . ) reactions with heme proteins and heme model compounds, with the goal of providing further insight into the related reactions occurring in mammalian biology.