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Dive into the research topics where Mark E. Arsenault is active.

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Featured researches published by Mark E. Arsenault.


Nature Structural & Molecular Biology | 2010

Single-molecule stepping and structural dynamics of myosin X

Yujie Sun; Osamu Sato; Felix Ruhnow; Mark E. Arsenault; Mitsuo Ikebe; Yale E. Goldman

Myosin X is an unconventional myosin with puzzling motility properties. We studied the motility of dimerized myosin X using the single-molecule fluorescence techniques polTIRF, FIONA and Parallax to measure the rotation angles and three-dimensional position of the molecule during its walk. It was found that Myosin X steps processively in a hand-over-hand manner following a left-handed helical path along both single actin filaments and bundles. Its step size and velocity are smaller on actin bundles than individual filaments, suggesting myosin X often steps onto neighboring filaments in a bundle. The data suggest that a previously postulated single α-helical domain mechanically extends the lever arm, which has three IQ motifs, and either the neck-tail hinge or the tail is flexible. These structural features, in conjunction with the membrane- and microtubule-binding domains, enable myosin X to perform multiple functions on varied actin structures in cells.


Physical Chemistry Chemical Physics | 2009

Using electrical and optical tweezers to facilitate studies of molecular motors

Mark E. Arsenault; Yujie Sun; Haim H. Bau; Yale E. Goldman

Dielectrophoresis was used to stretch and suspend actin filaments across a trench etched between two electrodes patterned on a glass slide. Optical tweezers were used to bring a motor protein-coated bead into close proximity to a pre-selected, suspended actin filament, facilitating the attachment of the myosin-coated bead to the filament. The clearance beneath the filament allowed the bead to move freely along and around its filamentous track, unhindered by solid surfaces. Using defocused images, the three-dimensional position of the bead was tracked as a function of time to obtain its trajectory. Experiments were carried out with myosin V and myosin X. Both motor proteins followed left-handed helical paths with the myosin X motor exhibiting a shorter pitch than the myosin V. The combined use of electrostatic and optical tweezers facilitates the preparation of motility assays with suspended tracks. Variants of this technique will enable higher complexity experiments in vitro to better understand the behavior of motors in cells.


Biophysical Journal | 2007

Confinement and Manipulation of Actin Filaments by Electric Fields

Mark E. Arsenault; Hui Zhao; Prashant K. Purohit; Yale E. Goldman; Haim H. Bau


International Journal of Non-linear Mechanics | 2008

The mechanics of short rod-like molecules in tension

Prashant K. Purohit; Mark E. Arsenault; Yale E. Goldman; Haim H. Bau


Physical Review E | 2010

Comparison of Brownian-dynamics-based estimates of polymer tension with direct force measurements

Mark E. Arsenault; Prashant K. Purohit; Yale E. Goldman; Henry Shuman; Haim H. Bau


Journal of biomolecular techniques | 2013

The Advantages of Micro-Cal™ Auto-iTC200 for Secondary Screening in a Fragment-based Drug Discovery Campaign.

Natalia Markova; Ronan O'Brien; Jessica Martinsson; Kenth Halberg; Martin Andersson; Martin Welin; Mark E. Arsenault


Biophysical Journal | 2011

Droplet Tracking for Single Molecule Confinement

Benjamin Gamari; Mark E. Arsenault; Peker Milas; Richard E. Buckman; Christophe Wilhelm; Lori S. Goldner


Biophysical Journal | 2011

Aluminum Phosphate Adsorption of Proteins Using Isothermal Titration Calorimetry

Ronan O'Brien; Verna Frasca; Mark E. Arsenault; Mary Jo Wojtusik


Biophysical Journal | 2010

Single Molecule Stepping and Structural Dynamics of Myosin X

Yujie Sun; Osamu Sato; Felix Ruhnow; Mark E. Arsenault; Mitsuo Ikebe; Yale E. Goldman


Biophysical Journal | 2009

A Novel Method For Investigating The Azimuthal Rotation Of Molecular Motors Utilizing Dielectrophoresis And Optical Tweezers

Mark E. Arsenault; Yujie Sun; Haim H. Bau; Yale E. Goldman

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Yale E. Goldman

University of Pennsylvania

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Haim H. Bau

University of Pennsylvania

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Hui Zhao

University of Pennsylvania

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Mitsuo Ikebe

University of Massachusetts Medical School

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Osamu Sato

University of Massachusetts Medical School

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Benjamin Gamari

George Washington University

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Henry Shuman

University of Pennsylvania

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