Mark E. Arsenault
University of Pennsylvania
Network
Latest external collaboration on country level. Dive into details by clicking on the dots.
Publication
Featured researches published by Mark E. Arsenault.
Nature Structural & Molecular Biology | 2010
Yujie Sun; Osamu Sato; Felix Ruhnow; Mark E. Arsenault; Mitsuo Ikebe; Yale E. Goldman
Myosin X is an unconventional myosin with puzzling motility properties. We studied the motility of dimerized myosin X using the single-molecule fluorescence techniques polTIRF, FIONA and Parallax to measure the rotation angles and three-dimensional position of the molecule during its walk. It was found that Myosin X steps processively in a hand-over-hand manner following a left-handed helical path along both single actin filaments and bundles. Its step size and velocity are smaller on actin bundles than individual filaments, suggesting myosin X often steps onto neighboring filaments in a bundle. The data suggest that a previously postulated single α-helical domain mechanically extends the lever arm, which has three IQ motifs, and either the neck-tail hinge or the tail is flexible. These structural features, in conjunction with the membrane- and microtubule-binding domains, enable myosin X to perform multiple functions on varied actin structures in cells.
Physical Chemistry Chemical Physics | 2009
Mark E. Arsenault; Yujie Sun; Haim H. Bau; Yale E. Goldman
Dielectrophoresis was used to stretch and suspend actin filaments across a trench etched between two electrodes patterned on a glass slide. Optical tweezers were used to bring a motor protein-coated bead into close proximity to a pre-selected, suspended actin filament, facilitating the attachment of the myosin-coated bead to the filament. The clearance beneath the filament allowed the bead to move freely along and around its filamentous track, unhindered by solid surfaces. Using defocused images, the three-dimensional position of the bead was tracked as a function of time to obtain its trajectory. Experiments were carried out with myosin V and myosin X. Both motor proteins followed left-handed helical paths with the myosin X motor exhibiting a shorter pitch than the myosin V. The combined use of electrostatic and optical tweezers facilitates the preparation of motility assays with suspended tracks. Variants of this technique will enable higher complexity experiments in vitro to better understand the behavior of motors in cells.
Biophysical Journal | 2007
Mark E. Arsenault; Hui Zhao; Prashant K. Purohit; Yale E. Goldman; Haim H. Bau
International Journal of Non-linear Mechanics | 2008
Prashant K. Purohit; Mark E. Arsenault; Yale E. Goldman; Haim H. Bau
Physical Review E | 2010
Mark E. Arsenault; Prashant K. Purohit; Yale E. Goldman; Henry Shuman; Haim H. Bau
Journal of biomolecular techniques | 2013
Natalia Markova; Ronan O'Brien; Jessica Martinsson; Kenth Halberg; Martin Andersson; Martin Welin; Mark E. Arsenault
Biophysical Journal | 2011
Benjamin Gamari; Mark E. Arsenault; Peker Milas; Richard E. Buckman; Christophe Wilhelm; Lori S. Goldner
Biophysical Journal | 2011
Ronan O'Brien; Verna Frasca; Mark E. Arsenault; Mary Jo Wojtusik
Biophysical Journal | 2010
Yujie Sun; Osamu Sato; Felix Ruhnow; Mark E. Arsenault; Mitsuo Ikebe; Yale E. Goldman
Biophysical Journal | 2009
Mark E. Arsenault; Yujie Sun; Haim H. Bau; Yale E. Goldman