Mark P. Rayson

Human gene for physical performance

A specific genetic factor that strongly influences human physical performance has not so far been reported, but here we show that a polymorphism in the gene encoding angiotensin-converting enzyme does just that. An ‘insertion’ allele of the gene is associated with elite endurance performance among high-altitude mountaineers. Also, after physical training, repetitive weight-lifting is improved eleven-fold in individuals homozygous for the ‘insertion’ allele compared with those homozygous for the ‘deletion’ allele.

Angiotensin-converting-enzyme gene insertion/deletion polymorphism and response to physical training.

BACKGROUND The function of local renin-angiotensin systems in skeletal muscle and adipose tissue remains largely unknown. A polymorphism of the human angiotensin converting enzyme (ACE) gene has been identified in which the insertion (I) rather than deletion (D) allele is associated with lower ACE activity in body tissues and increased response to some aspects of physical training. We studied the association between the ACE gene insertion or deletion polymorphism and changes in body composition related to an intensive exercise programme, to investigate the metabolic effects of local human renin-angiotensin systems. METHODS We used three independent methods (bioimpedance, multiple skinfold-thickness assessment of whole-body composition, magnetic resonance imaging of the mid-thigh) to study changes in body composition in young male army recruits over 10 weeks of intensive physical training. FINDINGS Participants with the II genotype had a greater anabolic response than those with one or more D alleles for fat mass (0.55 vs -0.20 kg, p=0.04 by bioimpedance) and non-fat mass (1.31 vs -0.15 kg, p=0.01 by bioimpedance). Changes in body morphology with training measured by the other methods were also dependent on genotype. INTERPRETATION II genotype, as a marker of low ACE activity in body tissues, may conserve a positive energy balance during rigorous training, which suggests enhanced metabolic efficiency. This finding may explain some of the survival and functional benefits of therapy with ACE inhibitors.

The ACE gene and muscle performance

Angiotensin-converting enzyme in human skeletal muscle can be encoded by either of two variants of the ACE gene, one of which carries an insertion of 287 base pairs. This longer allele gives rise to lower enzyme activity, and is associated with enhanced endurance performance and an anabolic response to intense exercise training. Here we examine training-related changes in the mechanical efficiency of human skeletal muscle (energy used per unit power output) and find that the presence of this ACE allele confers an enhanced mechanical efficiency in trained muscle.

Physiology: The ACE gene and muscle performance

Angiotensin-converting enzyme in human skeletal muscle can be encoded by either of two variants of the ACE gene, one of which carries an insertion of 287 base pairs. This longer allele gives rise to lower enzyme activity, and is associated with enhanced endurance performance and an anabolic response to intense exercise training. Here we examine training-related changes in the mechanical efficiency of human skeletal muscle (energy used per unit power output) and find that the presence of this ACE allele confers an enhanced mechanical efficiency in trained muscle.

The effect of cool water ingestion on gastrointestinal pill temperature

UNLABELLED Telemetric gastrointestinal (GI) temperature pills are now commonly used to measure core body temperature and could minimize the risk of heat illness while maximizing operational effectiveness in workers subject to high levels of thermal strain. PURPOSE To quantify the effect of repeated cool water ingestion on the accuracy of GI pill temperature. METHODS Ten operational firefighters ingested a pill to measure GI temperature (T1int) before overnight sleep. Two hours following breakfast and 11.5 h after ingesting T1int, the firefighters ingested a second pill (T2int) before performing 8.5 h of intermittent activity (repetitive cycles of 30 min of seated rest followed by 30 min of general firefighter duties). During the first 2 min of each 30-min rest period, the firefighters consumed 250 mL of chilled water (5-8 degrees C). RESULTS Water ingestion had a highly variable effect both within and between subjects in transiently (32 +/- 10 min) reducing the temperature of T2int in comparison with T1int. In general, this transient reduction in T2int became progressively smaller as time following ingestion increased. In some firefighters, the difference between T1int and T2int became negligible (+/- 0.1 degrees C) after 3 h, whereas in two others, large differences (peaking at 2.0 degrees C and 6.3 degrees C) were still observed when water was consumed 8 h after pill ingestion. CONCLUSION These results show that a GI pill ingested immediately prior to physical activity cannot be used to measure core body temperature accurately in all individuals during the following 8 h when cool fluids are regularly ingested. This makes GI temperature measurement unsuitable for workers who respond to emergency deployments when regular fluid consumption is recommended operational practice.

Characterization of the metabolic demands of simulated shipboard Royal Navy fire-fighting tasks

The purpose of this study was to quantify the metabolic demand of simulated shipboard fire-fighting procedures currently practised by men and women in the Royal Navy (RN) and to identify a minimum level of cardiovascular fitness commensurate with satisfactory performance. Thirty-four males (M) and 15 females (F) volunteered as subjects for this study (n = 49). Maximal oxygen uptake ([Vdot]O2max) and heart rate (fc max) of each subject was assessed during a standardized treadmill test. During the main trials, volunteers were randomly assigned to complete several 4-min simulated shipboard fire-fighting tasks (boundary cooling (BC), drum carry (DC), extinguisher carry (EC), hose run (HR), ladder climb (LC)), at a work rate that was endorsed as a minimum acceptable standard. Heart rate (fc) and oxygen uptake ([Vdot]O2) were recorded at 10-s intervals during rest, exercise and recovery. Participants completed all tasks within an allocated time with the exception of the DC task, where 11 subjects (all females) failed to maintain the endorsed work rate. The DC task elicited the highest (p< 0.01) group mean peak metabolic demand (PMD) in males (43 ml min-1 kg-1) and females (42 ml min-1 kg-1) who were able to maintain the endorsed work rate. The BC task elicited the lowest PMD (23 ml min-1 kg-1), whilst the remaining three tasks elicited a remarkably similar PMD of 38–39 ml min-1 kg-1. The human endurance limit while wearing a self-contained breathing apparatus (SCBA) dictates that RN personnel are only able to fire-fight for 20–30 min, while wearing a full fire-fighting ensemble (FFE) and performing a combination of the BC, HR and LC tasks, which have a group mean metabolic demand of 32.8 ml min-1 kg-1. Given that in healthy subjects fire-fighting can be sustained at a maximum work intensity of 80% [Vdot]O2max when wearing SCBA for this duration, it is recommended that all RN personnel achieve a [Vdot]O2max of 41 ml min-1 kg-1 as an absolute minimum standard. Subjects with a higher [Vdot]O2max than the above quoted minimum are able to complete the combination of tasks listed with greater metabolic efficiency and less fatigue.

Effects of basic training on material handling ability and physical fitness of British Army recruits.

No study has yet evaluated the efficacy of British Army basic training in improving material handling performance. Therefore, the purpose of this study was to evaluate the efficacy of the current British Army basic training in improving material handling performance and physical fitness. Forty-seven males (19.4 (3.2) years of age, 1753 (59) mm in height, 71.0 (9.6) kg in weight) and 10 females (21.5 (3.5) years, 1623 (45) mm, 62.5 (5.2) kg) served as subjects. Testing was carried out in the week prior to, and in the final week of, an 11-week basic training course. Maximal box lifts to two different heights, and repetitive lifting and carrying of a 10 kg load did not improve with training. Static (38 cm upright pull) and dynamic (incremental dynamic lift to 145 cm) lifting strength data concurred with the maximal box lift data in that no improvement was observed. Repetitive lifting and carrying of a 22 kg load improved (29.5%, p < 0.001), as did 3.2 km loaded march performance with 25 kg (15.7%, p < 0.001), but march performance with a 15 kg load did not. Predicted VO2max improved from 48.4 to 51.4 ml.kg-1.min-1, a change of 6.1% (p < 0.05). Fat-free mass increased by 0.9 kg (1.5%, p < 0.01), and body fat reduced by 2.7% of body mass (20.1%, p < 0.001), resulting in a loss of 1.2 kg of body mass (1.7%, p < 0.01). It is concluded that basic training in the British Army produces some favourable adaptations in recruits, especially in terms of aerobic fitness. However, the poor development of strength and material handling ability during training fails to improve the ability of soldiers to perform simulated military tasks, and it does little to reduce future injury risk while performing these tasks.

Resistance training and the enhancement of the gains in material-handling ability and physical fitness of British Army recruits during basic training

The aim was to evaluate the efficacy of a modified British Army basic training that included resistance training in improving material-handling performance and physical fitness, and to compare the modified training directly with the normal basic training. Forty-three males [19.2 (2.6) years of age, 1764 (72) mm in height, 73.0 (10.6) kg in mass] and nine females [19.1 (2.2) years, 1641 (67) mm, 62.0 (7.2) kg] performed the modified basic training. Testing occurred in the week before and in the final week of the 11-week basic training. Improvements with the modified training were observed for all six material-handling tests, including 8–12% for maximal box lifting, 15–19% for repetitive lifting and carrying and 9–17% for loaded marching (all p <0.01), and other established measures of aerobic fitness, strength and kinanthropometric characteristics. Significantly greater improvements were observed for the modified training compared with the normal training in maximal box lift to 1.45 m (12.4 versus 1.7%, p <0.01), 3.2 km loaded march performance with 15 kg (8.9 versus 3.6%, p <0.05), estimated fat-free mass (4.2 versus 1.5%, p <0.001), predicted [Vdot]O2max (1 min -1) (9.3 versus 4.1%, p <0.01) and dynamic lift to 1.45 m (15.5 versus 0.2%, p <0.001). It was concluded that the improvements in material-handling ability and other aspects of physical fitness brought about in recruits by British Army basic training can be enhanced by the use of a physical training programme that includes a carefully designed resistance training element. Of particular note are the improvements shown in performance on material-handling tasks that require muscular strength, as these represent many of the tasks that soldiers encounter in their military careers.

Physical demands of firefighter search and rescue in ambient environmental conditions

This study investigated the physiological responses and limitations to a simulated search and rescue scenario in a high-rise building under ambient conditions. Sixteen firefighters performed the scenario under four conditions: standard duration breathing apparatus (SDBA) and 45 mm hose; extended duration breathing apparatus (EDBA) and 45 mm hose; SDBA and 70 mm hose; EDBA and 70 mm hose. Core temperature, skin temperature and heart rate were monitored. In four of 32 trials the casualty was rescued; the remainder of the trials were terminated for safety, high core temperature or shortage of air. Final core temperature and heart rate were higher in the EDBA (39.1°C; 72% heart rate reserve (HRR)) than SDBA conditions (38.6°C; 67%HRR). No differences were observed between hose sizes. The scenario proved too onerous to complete successfully in the majority of cases. Replacing SDBA with EDBA eliminates air supply as a limiting factor, but brings with it challenges of managing thermal strain.

Ambient Temperature and the Pituitary Hormone Responses to Exercise in Humans

Pituitary hormones have an important role during exercise yet relatively little is known about the stimulus for their release. Body temperature progressively increases during prolonged steady‐state exercise in the heat and we have investigated the role that this may play in the release of prolactin, growth hormone and cortisol (as an indicator of adrenocorticotropic hormone) into the circulation. Fit young male subjects exercised at 73 %O2,max until volitional fatigue at 20 °C and at 35 °C (30 % relative humidity at both temperatures). Rectal temperature and mean skin temperature were monitored and blood samples analysed for lactate, glucose, cortisol, growth hormone and prolactin concentrations. During the first 20 min, core temperature rose continuously and to a similar extent at both temperatures, while mean skin temperature was approximately 4 °C lower during exercise in the cool. Blood glucose concentration was essentially constant throughout the period of exercise while lactate concentration increased in the first 10 min and then remained constant with very similar changes in the two exercise conditions. Prolactin and growth hormone concentrations both increased during the exercise period while the concentration of cortisol declined slightly before rising slightly over the 40 min period. Prolactin release was significantly greater when exercise was carried out in the heat while there was no difference in the release of growth hormone or cortisol in the two conditions. When plotted as a function of rectal temperature, growth hormone concentration showed a linear relationship which was the same at ambient temperatures of 35 °C and 20 °C. Prolactin concentration had a curvilinear relationship with rectal temperature and this differed markedly at the two ambient temperatures. Cortisol concentration showed no dependence on any measure of body temperature. Our results are consistent with some aspect of body temperature being a stimulus for growth hormone and prolactin secretion; however, the precise mechanism clearly differs between the two hormones and we suggest that skin temperature modulates prolactin release, but does not affect the release of growth hormone.