Marta Revilla

Implementation of the European Marine Strategy Framework Directive: A methodological approach for the assessment of environmental status, from the Basque Country (Bay of Biscay)

The implementation of the Marine Strategy Framework Directive (MSFD) is directing European marine research towards the coordinated and integrated assessment of sea environmental status, following the ecosystem-based approach. The MSFD uses a set of 11 descriptors which, together, summarise the way in which the whole system functions. As such, the European Commission has proposed an extensive set of indicators, to assess environmental status. Hence, taking account of the large amount of data available for the Basque coast (southern Bay of Biscay), together with a recent proposal for assessment within the MSFD, an integrated environmental status assessment approach is developed (for the first time) in this contribution. The strengths and weaknesses of the method, combined with proposals from the MSFD, are discussed.

Using multiple ecosystem components, in assessing ecological status in Spanish (Basque Country) Atlantic marine waters

The European Water Framework and Marine Strategy Directives relate to the assessment of ecological quality, within estuarine and coastal systems. This legislation requires quality to be defined in an integrative way, using several biological elements (phytoplankton, benthos, algae, phanerogams, and fishes), together with physico-chemical elements (including pollutants). This contribution describes a methodology that integrates all of this information into a unique quality assessment for 51 stations from 18 water bodies, within the Basque Country. These water bodies are distributed into four typologies, including soft-bottom coastal areas and three types of estuaries. For each station, decision trees were used to integrate (i) water, sediment and biomonitor chemical data to achieve an integrated physico-chemical assessment and (ii) multiple biological ecosystem elements into an integrated biological assessment. Depending on the availability of ecological quality ratios or global quality values, different integration schemes were used to combine station assessments into water body assessments on a single scale. Several examples from each element have been selected, to illustrate their responses to different pressures; likewise, to establish how the assessed integrated quality has changed, over time. The results made biological and ecological sense and physico-chemical improvements were often correlated with improvements in the quality of benthos and fishes. These tools permit policy makers and managers to take decisions, based upon scientific knowledge, in water management, regarding the mitigation of human pressures and associated recovery processes.

Primary plankton production, respiration and nitrification in a shallow temperate estuary during summer

Abstract Patterns of longitudinal and temporal variation in rates of primary production, respiration and nitrification were investigated in the Urdaibai estuary during August 1994. Rates of primary production, as measured with the 14C technique, varied between 0.002 and 2.828 mg C l−1 d−1. 14C and oxygen based rates of primary production showed a good correlation, but photosynthetic quotients (mean PQ of 2.2) were higher than expected from considerations of the N source used by the algae. Chlorophyll a biomass showed an inverse correlation with salinity and tidal flushing is hypothesized to be a major controlling factor of its spatial variability. Temporal variations in chlorophyll a concentration were associated primarily to changes in the lunar cycle and following rain events. Rates of community respiration varied between 0.154 and 6.857 mg O2 l−1 d−1 and were significantly and positively correlated with both bacterial abundance and chlorophyll a concentration. The relationship between respiration and primary production suggested that respiration rates based on substrates not associated with the autochthonous pelagic primary production increased greatly from the outer to the inner estuary and that the consumption of autotrophic production by heterotrophs in the water column was higher in the less productive outer estuary than in highly productive intermediate and inner areas. Except at the innermost station, temporal variations in respiration rates followed variations in temperature. The ratio of gross primary production to respiration ( GP R ) showed marked spatial and temporal variations, ranging from 0.24 to 17.94. Rates of nitrification, measured as allylthiourea sensitive rates of CO2 fixation, varied from undetectable levels to 9.3 μg C l−1 d−1.In general, highest rates of nitrification were observed at the inner estuary under conditions of elevated ammonia concentration, high bacterial abundance and high turbidity. Rates of nitrification were frequently associated with the > 3 μm size fraction. Oxygen consumed in nitrification was estimated to account on average for 4.7 ± 8.3% of total oxygen consumption.

Water quality assessment using satellite-derived chlorophyll-a within the European directives, in the southeastern Bay of Biscay

The implementation of water quality European Directives requires an intensification of water quality monitoring, within the limits of the Exclusive Economic Zone. Remote sensing technologies can provide a valuable tool for frequent, synoptic, water-quality observations, over large areas. The aim of this study is to assess the ecological status of Basque coastal water bodies using satellite imagery from MODIS sensor, together with optical and chlorophyll-ain situ measurements. Thus, sea surface satellite-derived chl-a algorithms, the OC3 M, OC5 and a Local empirical algorithm, were compared against in situ measurements using satellite in situ match-ups, 90th Percentile (P90) monthly values for the 2005-2010 period. The OC5 algorithm corresponded most accurately with in situ measurements performed in the area, hence, it was selected. A P90 chlorophyll-a map was created with this algorithm to apply the classification scheme required by the directives. The classification of water bodies, based upon satellite-derived chlorophyll-a, could improve considerably the assessment of water quality.

Phytoplankton blooms in the Urdaibai estuary during summer: Physico-chemical conditions and taxa involved

In the Urdaibai estuary, despite its small volume in relation to the tidal prism, phytoplankton grows massively in the upper and intermediate zones of the estuary during summer when rainfall is low to moderate. Data obtained in 23 sampling transects along the longitudinal axis of the estuary, undertaken in July 1993 and August 1994, showed three distinct zones in terms of the phytoplankton species composition: in the upper zone the phytoplankton assemblage was dominated by the diatoms Cyclotella spp., the dinoflagellate Glenodinium foliaceum, cryptophytes and euglenophytes; in the intermediate zone the diatom Chaetoceros ceratosporus, the dinoflagellates Peridinium quinquecorne and Prorocentrum minimum and cryptophytes were the most abundant; in the lower zone diatoms such as Leptocylindricus danicus and Skeletonema costatum together with the dinoflagellates Prorocentrum spp. were dominant. The distribution and abundance of these phytoplankton forms is discussed in relation to variations in salinity, water column stratification, nutrients and temperature, which in turn vary mainly as a function of meteorological conditions (i.e. rainfall, irradiance) and tidal amplitude. The longitudinal distribution of phytoplankton cells suggests that the diatoms Cyclotella spp. and Chaetoceros ceratosporus undergo a greater dispersion along the estuary during rain events than the flagellates, thus contributing to the transport of silica to the outer areas of the estuary.

Microplankton metabolism along a trophic gradient in a shallow-temperate estuary

Microplankton metabolism was studied in the Urdaibai estuary from June 1996 to May 1997 to investigate the factors that control the autotrophic-heterotrophic balance in three zones of contrasting trophic status. Gross primary production (GPP) and dark oxygen consumption (R) were measured from the upper eutrophic reaches to the lower marine segment for unfractionated samples as well as for nanoplankton (<20 μm) and ultraplankton (<5 μm). Microplankton composition differed in the three segments and the peaks of abundance were uncoupled. Microplankton biomass both as carbon content and chlorophylla concentration decreased seaward. Size-fractionated metabolism was to a great extent conducted by the dominant species in the upper and middle reaches, where GPP and R rates were frequently coupled. In the lower marine estuary GPP experienced marked seasonal changes while R rates were rather constant and uncoupled to GPP. In the upper and lower estuary the gross primary production:respiration (P:R) ratio of the nonfractionated samples were >1 only during peak values of production, whilst in the middle estuary they were almost always ≥1. Ultraplankton was generally heterotrophic in the three zones. The relative importance of the <5 μm fraction increased seaward for both GPP and R rates. This size class was responsible for most of the oxygen consumption in the middle and lower estuary, while in the upper estuary the 5–20 μm size fraction was also relevant. During periods of enhanced phytoplankton growth, less than 20% of the total GPP was instantaneously respired in the three segments. In periods between peaks, only in the middle estuary was some percentage of the GPP not instantaneously processed. The percentage of the fractionated primary production respired in each size fraction was highly variable, although the highest values were found in the <5 μm for all stations.

Eutrophication Assessment in Basque Estuaries: Comparing a North American and a European Method

Eutrophication in marine ecosystems is an important problem that requires an accurate assessment. Although Basque estuaries (northern Spain) have historically been under high anthropogenic pressure, no specific eutrophication assessment method had been applied in these waters. In this study, a method employed in the Basque Country (BC) to assess the “risk of failing to achieve good ecological status” under the requirements of the Water Framework Directive (WFD) was adapted to exclusively assess the risk of eutrophication. This method is based on the driver–pressure–state–impact–response approach. The results from this method (called WFD-BC method) were compared to the results from Assessment of Estuarine Trophic Status (ASSETS; a specific method developed in the US to assess estuarine trophic status in a pressure–state–response approach). The nutrient pressure was better characterized with the WFD-BC method due to the local hydrographic conditions (i.e., small and river-influenced estuaries) that were not well accommodated by the ASSETS method. In contrast, the WFD-BC results for assessment of state generally reflected worse conditions than the results from the ASSETS method due to the different indicators employed and the way these are integrated in the WFD-BC method. Overall, the WFD-BC method showed a good potential to assess eutrophication. However, to improve it, a lower weight for the benthos and macroalgae is recommended for evaluating state.

Seasonal variability in gonad development in the sea urchin (Paracentrotus lividus) on the Basque coast (Southeastern Bay of Biscay).

The main limiting factor to carrying out toxicological bioassays with sea urchin larvae is the restricted period for the availability of viable gametes. Although studies have been undertaken of the reproductive cycle of the sea urchin for several areas of the Bay of Biscay, only limited information exists for the southeasternmost area (the Basque coast). Furthermore, this geographical zone presents some particular environmental conditions, e.g., relatively warm waters. In this study, the gonad state of a population of Paracentrotus lividus, settled on the rocky shores of the city of Donostia-San Sebastian have been monitored. These sea urchins are observed to behave like a typical Atlantic population, with generally high gonad indices and a single yearly main spawning period, between April and May. Further, females show a shorter spawning period than males, becoming the limiting organisms for the availability of larvae.

Sources of uncertainty in assessment of marine phytoplankton communities

Characterisation of phytoplankton communities is important for classification of the ecological status of marine waters. In order to design a monitoring programme, it is important to know what degree of variation in the measurements occur at each level (water body, station and sample), so that resources can be spent in a way that maximise the precision of the measured parameters. Seven European water bodies were sampled to assess the variation in pigment concentrations and population densities attributed to water body, station and sample levels. It was found that the main proportion of the variation between pigment measurements was explained by the variation between stations (12–91% of variation) followed by the variation between water bodies (0–89% of variation). For measurements of population density, the main proportion of the variation between densities of cells recorded was explained by the variation between the taxonomists counting the samples (61%), whilst the main proportion of the variation between numbers of taxa recorded was explained by the variation between water bodies (83%). When the cell density of the nine dominant classes were analysed separately, the main proportion of variation was explained at the water body level for all but two class.

'The past is the future of the present': Learning from long-time series of marine monitoring.

Using a long-term (1995-2014) monitoring network, from 51 sampling stations in estuaries and coasts of the Basque Country (Bay of Biscay), the objective of this investigation was to assess the responsiveness of 83 variables in water (18), sediments (27), biota (26), phytoplankton (2), macroinvertebrates (5) and fishes (5) to different human pressures and management actions. We used a total of 3247 series of data to analyse trends of improvement and worsening in quality. In a high percentage of the cases, the management actions taken have resulted in positive effects in the environment, as shown by the trend analysis in this investigation. Overall, much more trends of improvement than of worsening have been observed; this is true for almost all the media and biological components studied, with the exception of phytoplankton; and it applies as well to almost all the stations and water bodies, with the exception of those corresponding to areas with water treatment pending of accomplishment. In estuaries with decreasing human pressures during the period, the percentage of series showing quality improvement was higher (approx. 30%) than those showing worsening of quality (12%). Moreover, in those water bodies showing an increase of pressure, variables which can be considered indicators of anthropogenic effects showed negative trends (quality worsening). On the other hand, some of the variables analysed were more affected by natural variability than by changes in pressures. That was the case of silicate, nitrate and suspended solids, which followed trends correlated to salinity, which, in turn, was related to the rainfall regime during the study period.