Martha Maria Gebhard

Autophagy mediates survival of pancreatic tumour‐initiating cells in a hypoxic microenvironment

Involvement of dysregulated autophagy in cancer growth and progression has been shown in different tumour entities, including pancreatic ductal adenocarcinoma (PDA). PDA is an extremely aggressive tumour characterized by a small population of highly therapy‐resistant cancer stem cells (CSCs) capable of self‐renewal and migration. We examined whether autophagy might be involved in the survival of CSCs despite nutrition and oxygen deprivation typical for the hypoxic tumour microenvironment of PDA. Immunohistochemistry revealed that markers for hypoxia, CSCs and autophagy are co‐expressed in patient‐derived tissue of PDA. Hypoxia starvation (H/S) enhanced clonogenic survival and migration of established pancreatic cancer cells with stem‐like properties (CSC

Cigarette smoke enhances ethanol-induced pancreatic injury.

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Influence of lidocaine on endotoxin-induced leukocyte-endothelial cell adhesion and macromolecular leakage in vivo.

, while pancreatic tumour cells with fewer stem cell markers (CSC

The selective Cox-2 inhibitor Celecoxib suppresses angiogenesis and growth of secondary bone tumors: An intravital microscopy study in mice

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Ketamine attenuates endotoxin-induced leukocyte adherence in rat mesenteric venules

did not survive these conditions. Electron microscopy revealed more advanced autophagic vesicles in CSC

N-acetylcysteine attenuates endotoxin-induced leukocyte-endothelial cell adhesion and macromolecular leakage in vivo.

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Dopexamine attenuates endotoxin-induced microcirculatory changes in rat mesentery: role of beta2 adrenoceptors.

cells, which exhibited higher expression of autophagy‐related genes under normoxic conditions and relative to CSC

Leukocyte-independent plasma extravasation during endotoxemia.

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Characterization and reduction of ischemia/reperfusion injury after experimental pancreas transplantation☆☆☆

cells, as found by RT‐PCR and western blot analysis. LC3 was already fully converted to the active LC3‐II form in both cell lines, as evaluated by western blot and detection of accumulated GFP‐LC3 protein by fluorescence microscopy. H/S increased formation of autophagic and acid vesicles, as well as expression of autophagy‐related genes, to a higher extent in CSC

Late remote ischemic preconditioning in rat muscle and adipocutaneous flap models

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