Martin Paul Robinson

Analytical formulation for the shielding effectiveness of enclosures with apertures

An analytical formulation has been developed for the shielding effectiveness of a rectangular enclosure with an aperture. Both the magnetic and electric shielding may be calculated as a function of frequency, enclosure dimensions, aperture dimensions and position within the enclosure. Theoretical values of shielding effectiveness are in good agreement with measurements. The theory has been extended to account for circular apertures, multiple apertures, and the effect of the enclosure contents.

Vitrification media: toxicity, permeability, and dielectric properties ☆

The aim of this study was to select a cryoprotectant for use in attempts to preserve tissues and organs by vitrification. The first step was to select a cell line with which to compare the toxicity of a range of commonly used cryoprotectants. An immortal vascular endothelial cell (ECV304) was exposed to vitrifying concentrations of four cryoprotectants: dimethyl sulfoxide (Me(2)SO; 45% w/w); 2,3 butanediol (BD; 32%); 1,2-propanediol (PD; 45%); and ethanediol (ED; 45%). Three times of exposure (1, 3, and 9 min) and two temperatures (22 and 2-4 degrees C) were studied. After removal of the cryoprotectant, the ability of the cells to adhere and divide in culture over a 2-day period was measured and expressed as a Cell Survival Index (CSI). There was no measurable loss of cells after exposure to the four cryoprotectants but 3-min exposure to BD, PD, or Me(2)SO at room temperature completely destroyed the ability of the cells to adhere and divide in culture. In contrast, exposure to all four cryoprotectants at 2-4 degrees C for up to 9 min permitted the retention of significant cell function, the CSIs, as a proportion of control, being 76.3+/-7.0% for BD, 63.6+/-7.1% for PD, 37.0+/-4.1 for Me(2)SO, and 33.2+/-3.0 for ED. The permeability properties of the cells for these four cryoprotectants was also measured at each temperature. Permeability to water was high, L(p) approximately equal 10(-7) cm/s/atm at 2-4 degrees C with all the cryoprotectants, but there were substantial differences in solute permeability: BD and PD were the most permeable at 2-4 degrees C (P(s)=4.1 and 3.0 x 10(-6) cm/s, respectively). Equilibration of intracellular cryoprotectant concentration was rapid, due in part to high water permeability; the cells were approximately 80% of their physiological volume after 10 min. Treatment at 2-4 degrees C with BD was the least damaging, but PD was not significantly worse. Exposure to vitrifying concentrations of ED and Me(2)SO, even at 2-4 degrees C, was severely damaging. Segments of rabbit carotid artery were treated with vitrifying concentrations of each of the two most favorable cryoprotectants, BD and PD, for 9 min. It was shown that each cryoprotectant reduced smooth muscle maximum contractility to a similar extent and abolished the acetylcholine response. However, vital staining revealed that exposure to BD also caused substantial damage to the endothelial lining, whereas the endothelium was completely intact after PD exposure, raising the possibility that the effect of PD on NO release may be reversible. In later stages of this project it is planned to use dielectric heating to rewarm the tissues and thereby avoid devitrification. The effects of each cryoprotectant on this mode of heating was therefore studied. Gelatin spheres containing vitrifiable concentrations of each cryoprotectant were rewarmed from -60 degrees C in a radiofrequency applicator. Because the uniformity of heating is related to the dielectric properties of the material, these properties were also measured. PD was the most suitable. These physical measurements, combined with the measurements of toxicity and permeability, indicate that PD is the most favorable cryoprotectant of those tested for use in subsequent stages of this study.

A fast multiple mode intermediate level circuit model for the prediction of shielding effectiveness of a rectangular box containing a rectangular aperture

This paper presents an intermediate level circuit model (ILCM) for the prediction of the shielding effectiveness (SE) of a rectangular box containing a rectangular aperture, irradiated by a plane wave. The ILCM takes into account multiple waveguide modes, and is thus suitable for use at high frequencies and/or relatively large boxes. Inter-mode coupling and reradiation from the aperture are taken into account. The aperture may be positioned anywhere in the front face of the box, and the SE at any point within the box may be found. The model is presented in such a way that existing ILCM techniques for modelling elements such as monopoles, dipoles, loops, or transmission lines may be seamlessly incorporated into the circuit model. Solution times using the ILCM technique are three orders of magnitude less than those required by traditional numerical methods such as FDTD, TLM, or MoM. Accuracy however is not significantly compromised. Comparing the circuit model with TLM over nine data sets from 4 MHz to 3 GHz resulted in an rms difference of 7.70 dB and mean absolute difference of 5.55 dB in the predicted SE values.

Electromagnetic re-warming of cryopreserved tissues: effect of choice of cryoprotectant and sample shape on uniformity of heating.

A method that has been proposed for the cryopreservation of tissues and organs is to add a cryoprotective agent (CPA) in sufficient concentration to allow vitrification, and to use rapid electromagnetic heating to prevent the formation of ice crystals during the re-warming. We have compared the physical and biological properties of four CPAs, measuring the speed and uniformity of heating in a 36 mm sphere placed in a 434 MHz applicator, and the toxicity to ECV304 endothelial cells. Ethanediol and dimethyl sulfoxide were found to be suitable for rapid, uniform heating but toxic to the endothelial cells at vitrifying concentrations. Butane-2,3-diol was less toxic, but the heating patterns were unacceptably non-uniform. Propane-1,2-diol was not significantly more toxic than butane-2,3-diol, and did allow uniform heating. It is therefore the best choice of CPA for the vitrification of tissues. We have shown that the uniformity of heating correlates with the dielectric properties of the perfusate. Furthermore, we have shown that uniform heating is feasible in non-spherical samples provided they are approximately ellipsoidal.

Rapid electromagnetic warming of cells and tissues

Describes a system for thawing frozen cell suspensions and tissues by electromagnetic absorption. A 25-ml sample is heated in a cylindrical resonant cavity, which is excited in three modes all close to 434 MHz. Maximum warming rates are over 10/spl deg/C/s (600 C/min), and a frozen sample may be brought from -65/spl deg/C to room temperature in <30 s, with final spatial differences of <20/spl deg/C. Samples may be frozen externally, or cooled within the cavity at typically 1/spl deg/C/min. The authors have also used the resonant cavity to measure the permittivity and conductivity of the sample at temperatures from -83/spl deg/C to +8/spl deg/C. By measuring the heat capacity of the sample, the authors have calculated the power deposited in it as a function of its temperature. The system is currently being used to investigate the effect of warming rate on cell survival.

Improved determination of Q-factor and resonant frequency by a quadratic curve-fitting method

The Q-factor and peak frequency of resonant phenomena give useful information about the propagation and storage of energy in an electronic system and therefore its electromagnetic compatibility performance. However, the calculation of Q by linear interpolation of a discrete frequency response to obtain the half-power bandwidth can give inaccurate results, particularly if the data are noisy or the frequency resolution is low. We describe a more accurate method that makes use of the Lorentzian shape of the resonant peaks and involves fitting a second-order polynomial to the reciprocal power plotted against angular frequency. We demonstrate that this new method requires less than one quarter the number of frequency points as the linear method to give comparable accuracy in Q. The new method also gives comparable accuracy for signal-to-noise ratios that are approximately 8 dB greater. It is also more accurate for determination of peak frequency. Examples are given both from measured frequency responses and from simulated data obtained by the transmission line matrix method.

Interference to medical equipment from mobile phones

Cellular mobile phones may interfere with hospital equipment. We irradiated five representative pieces of equipment using simulated phone signals and frequencies. Two (an oximeter and a syringe pump) were immune to electric fields of up to 40 V m-1. The most susceptible was a physiological monitor which showed effects at 10 V m-1. None of the equipment was affected by fields that could be produced at over one metre from a 2 W mobile phone.

New materials for dielectric simulation of tissues

Two novel materials have been developed to simulate the dielectric properties of human muscle and fat at 1000 MHz. These materials, which are based on ethanediol set with gelatine, are easily made and have good mechanical properties. The complex permittivity, epsilon *, of each material was measured with an open-ended coaxial sensor in conjunction with an automatic network analyser. Values of epsilon * at 1000 MHz and room temperature (49.4-24.4j for the muscle-equivalent material, 8.2-3.6j for the fat-equivalent) compared well with literature values for tissue at body temperature. Thermal properties were also considered. Specific heat capacities of the materials at room temperature were measured by differential scanning calorimetry and found to be very close to values predicted by theory. Densities were also measured and the thermal conductivities estimated. For comparison, new measurements were made of the specific heat and density of human muscle and fat at body temperature.

Measurement of the efficiency of cell membrane electroporation using pulsed ac fields.

Electroporation is a long-established technique used to deliver molecules to cells. Most in vivo electroporation protocols entail applying square-wave, or monotonically-decreasing pulses but relatively few have explored the use of pulsed ac fields. This study measures the efficiency of electroporation in human kidney embryonal cells, using pulsed ac electric fields of peak amplitude 30-200 kV m(-1). The results indicate that optimum electroporation efficiencies of up to 70% can be achieved using pulses at frequencies of 20-160 kHz. Increasing the field strength results in higher electroporation efficiency, but also increases cell kill. This study confirms that efficient electroporation may be achieved using pulsed ac fields. This finding raises the possibility of a wider range of clinical and laboratory applications based on ac technology and avoiding the use of invasive needle electrodes.

Radio frequency electromagnetic fields in large conducting enclosures: effects of apertures and human bodies on propagation and field-statistics

Radio frequency propagation in an electrically large resonant chamber (a screened room) was simulated by two models: a statistical combination of multiple resonant modes and a computational electromagnetic simulation [the transmission line matrix (TLM) method]. The purpose of this work was to investigate the effects of passengers and windows on electromagnetic fields (EMF) in aircraft and other vehicles. Comparison of the multimode models with measurements made in a screened room showed that as the electromagnetic losses increased, the transmission between two internal antennas was reduced, and there were fewer turning points in its frequency response. The autocorrelation of this frequency response provided a useful estimate of the composite Q-factor of the resonances and showed that the Q of the chamber was reduced from a value of the order of 10 000 when emptied to 1000 when windows were added and when filled with people to 100. The TLM simulation provided further useful information about the statistical variation of electric field strength with position.