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Featured researches published by Martina Mutter.


Biotechnology Journal | 2013

Continuous purification of antibodies from cell culture supernatant with aqueous two-phase systems: From concept to process

Paula A. J. Rosa; Ana M. Azevedo; Sven Sommerfeld; Martina Mutter; Werner Bäcker; M. Raquel Aires‐Barros

An aqueous two-phase extraction (ATPE) process based on a PEG/phosphate system was developed for the capture of human immunoglobulin G and successfully applied to a Chinese hamster ovary and a PER.C6® cell supernatant. A continuous ATPE process incorporating three different steps (extraction, back-extraction, and washing) was set up and validated in a pump mixer-settler battery. Most of the higher molecular weight cell supernatant impurities were removed during the extraction step, while most of the lower molecular weight impurities were removed during the subsequent steps. A global recovery yield of 80% and a final protein purity of more than 99% were obtained for the IgG purification from a CHO cell supernatant, representing a 155-fold reduction in the protein/IgG ratio. For the purification of IgG from a PER.C6® cell supernatant, a global recovery yield of 100%, and a host cell protein purity were attained, representing a 22-fold reduction in the host cell protein/IgG ratio. These results, thus, open promising perspectives for the application of the developed ATPE process as a platform for the capture of antibodies. In fact, this new process has shown the ability to successfully recover and purify different antibodies from distinct cell culture supernatants. This technology can also overcome some of the limitations encountered using the typical chromatographic processes, besides inherent advantages of scalability, process integration, capability of continuous operation, and economic feasibility.


Chemical Papers | 2008

Computer-aided process design of affinity membrane adsorbers: a case study on antibodies capturing

Peter van Beijeren; Peter Kreis; Achim Hoffmann; Martina Mutter; Sven Sommerfeld; Werner Bäcker; Andrzej Górak

Design of affinity membrane adsorbers for the purification of biomolecules requires a consideration of loading, washing, and elution. Modelling and simulation of membrane adsorbers in literature is, however, strongly focused on the loading step. Therefore, in this work, a complete process model which takes all the different steps into account was developed. Breakthrough experiments in which human IgG was captured onto and eluted from Sartobind Protein A downscale modules were used for model validation and for estimation of the required model parameters. The experimentally observed breakthrough curves were independent of the applied flow rate and from these results linear correlations between lumped kinetic parameters and linear velocity were determined. During elution, desorption was best described by an irreversible reaction of first order in H+ concentration. Applicability of the developed model to computer-aided design was illustrated through a process analysis study in which the influence of the amount of loaded protein per cycle on the process yield and productivity was investigated.


Journal of Biotechnology | 2009

Application of aqueous two-phase systems to antibody purification: a multi-stage approach.

P.A.J. Rosa; Ana M. Azevedo; Sven Sommerfeld; Martina Mutter; M.R. Aires-Barros; Werner Bäcker


Archive | 2004

Verfahren zum Abtrennen von gelösten oder kolloidalen Feststoffen aus nicht wässrigen Lösungen

Gregor Dudziak; Andreas Nickel; Kerstin Baumarth; Martina Mutter; Olaf Stange; Rafael Warsitz


Archive | 2003

Device and method for preparative electrophoresis

Gregor Dudziak; Andreas Nickel; Martina Mutter; Kerstin Baumarth


Archive | 2004

Process for separating dissolved or colloidal solids from a nonaqueous solvent

Gregor Dudziak; Andreas Nickel; Kerstin Baumarth; Martina Mutter; Olaf Strange; Rafael Warsitz


Archive | 2009

Method for purifying therapeutic proteins by means of multi-stage extraction

Werner Bäcker; Sven Sommerfeld; Martina Mutter; De Jesus Rose Paula Alexandra Albuquerque; Santos Aires-Barosl Maria Raquel Murias Dos; Mata Pires De Azevedo Ana Margarida Nunes Da


Archive | 2005

Device and process for membrane electrophoresis and electrofiltration

Ralf Lausch; Oscar-Werner Reif; Ulrich Grummert; Stefan Haufe; Holger Linne; Andre Pastor; Gregor Dudziak; Andreas Nickel; Martina Mutter; Michael Traving


Archive | 2009

Method for purifying therapeutic proteins

Werner Bäcker; Sven Sommerfeld; Martina Mutter; De Jesus Rose Paula Alexandra Albuquerque; Santos Aires-Barosl Maria Raquel Murias Dos; Mata Pires De Azevedo Ana Margarida Nunes Da


Archive | 2009

Verfahren zur Reinigung therapeutischer Proteine

Sven Sommerfeld; Werner Bäcker; Martina Mutter; Paula Alexandra Albuquerque de Jesus Rosa; Maria Raquel Murias dos Santos Aires-Barosl; Ana Margarida Nunes da Mata Pires de Azevedo

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