Martina Mutter
Bayer
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Publication
Featured researches published by Martina Mutter.
Biotechnology Journal | 2013
Paula A. J. Rosa; Ana M. Azevedo; Sven Sommerfeld; Martina Mutter; Werner Bäcker; M. Raquel Aires‐Barros
An aqueous two-phase extraction (ATPE) process based on a PEG/phosphate system was developed for the capture of human immunoglobulin G and successfully applied to a Chinese hamster ovary and a PER.C6® cell supernatant. A continuous ATPE process incorporating three different steps (extraction, back-extraction, and washing) was set up and validated in a pump mixer-settler battery. Most of the higher molecular weight cell supernatant impurities were removed during the extraction step, while most of the lower molecular weight impurities were removed during the subsequent steps. A global recovery yield of 80% and a final protein purity of more than 99% were obtained for the IgG purification from a CHO cell supernatant, representing a 155-fold reduction in the protein/IgG ratio. For the purification of IgG from a PER.C6® cell supernatant, a global recovery yield of 100%, and a host cell protein purity were attained, representing a 22-fold reduction in the host cell protein/IgG ratio. These results, thus, open promising perspectives for the application of the developed ATPE process as a platform for the capture of antibodies. In fact, this new process has shown the ability to successfully recover and purify different antibodies from distinct cell culture supernatants. This technology can also overcome some of the limitations encountered using the typical chromatographic processes, besides inherent advantages of scalability, process integration, capability of continuous operation, and economic feasibility.
Chemical Papers | 2008
Peter van Beijeren; Peter Kreis; Achim Hoffmann; Martina Mutter; Sven Sommerfeld; Werner Bäcker; Andrzej Górak
Design of affinity membrane adsorbers for the purification of biomolecules requires a consideration of loading, washing, and elution. Modelling and simulation of membrane adsorbers in literature is, however, strongly focused on the loading step. Therefore, in this work, a complete process model which takes all the different steps into account was developed. Breakthrough experiments in which human IgG was captured onto and eluted from Sartobind Protein A downscale modules were used for model validation and for estimation of the required model parameters. The experimentally observed breakthrough curves were independent of the applied flow rate and from these results linear correlations between lumped kinetic parameters and linear velocity were determined. During elution, desorption was best described by an irreversible reaction of first order in H+ concentration. Applicability of the developed model to computer-aided design was illustrated through a process analysis study in which the influence of the amount of loaded protein per cycle on the process yield and productivity was investigated.
Journal of Biotechnology | 2009
P.A.J. Rosa; Ana M. Azevedo; Sven Sommerfeld; Martina Mutter; M.R. Aires-Barros; Werner Bäcker
Archive | 2004
Gregor Dudziak; Andreas Nickel; Kerstin Baumarth; Martina Mutter; Olaf Stange; Rafael Warsitz
Archive | 2003
Gregor Dudziak; Andreas Nickel; Martina Mutter; Kerstin Baumarth
Archive | 2004
Gregor Dudziak; Andreas Nickel; Kerstin Baumarth; Martina Mutter; Olaf Strange; Rafael Warsitz
Archive | 2009
Werner Bäcker; Sven Sommerfeld; Martina Mutter; De Jesus Rose Paula Alexandra Albuquerque; Santos Aires-Barosl Maria Raquel Murias Dos; Mata Pires De Azevedo Ana Margarida Nunes Da
Archive | 2005
Ralf Lausch; Oscar-Werner Reif; Ulrich Grummert; Stefan Haufe; Holger Linne; Andre Pastor; Gregor Dudziak; Andreas Nickel; Martina Mutter; Michael Traving
Archive | 2009
Werner Bäcker; Sven Sommerfeld; Martina Mutter; De Jesus Rose Paula Alexandra Albuquerque; Santos Aires-Barosl Maria Raquel Murias Dos; Mata Pires De Azevedo Ana Margarida Nunes Da
Archive | 2009
Sven Sommerfeld; Werner Bäcker; Martina Mutter; Paula Alexandra Albuquerque de Jesus Rosa; Maria Raquel Murias dos Santos Aires-Barosl; Ana Margarida Nunes da Mata Pires de Azevedo