Mary Anne Tafuri

Cleaning the dead: Neolithic ritual processing of human bone at Scaloria Cave, Italy

Abstract Detailed taphonomic and skeletal analyses document the diverse and often unusual burial practices employed by European Neolithic populations. In the Upper Chamber at Scaloria Cave in southern Italy, the remains of some two dozen individuals had been subjected to careful and systematic defleshing and disarticulation involving cutting and scraping with stone tools, which had left their marks on the bones. In some cases these were not complete bodies but parts of bodies that had been brought to the cave from the surrounding area. The fragmented and commingled burial layer that resulted from these activities indicates complex secondary burial rites effecting the transition from entirely living to entirely dead individuals.

Suggested guidelines for invasive sampling of hominid remains

The last few years have witnessed remarkable technical developments in paleoanthropology. On the one hand, accurate imaging techniques have limited the need to access actual specimens. On the other hand, direct dating, isotopic studies, and the study of ancient DNA, proteins, and microstructures have experienced great technical improvements but still require a degree of invasive sampling. The power of these invasive approaches for answering important questions in evolutionary anthropology brings forward the question of how to balance preservation of fossil hominid remains for the future against the application of current scientific analyses. In order to address these issues, a workshop was hosted by the Max-Planck Institute for Evolutionary Anthropology in Leipzig on April 26– 27, 2007 where the issues that emanate from the need for sampling of hominid remains versus the need for preservation of specimens for the future were discussed. At the end of the meeting, the participants produced a set of recommendations that might be useful to museums and other institutions as well as scientists that have to make decisions on requests for invasive sampling of hominid remains.

First Strontium Isotope Evidence of Mobility in the Neolithic of Southern France

AbstractThis study presents the first 87Sr/ 86Sr isotope results obtained on Neolithic humans from Southern France. These analyses aimed at exploring patterns of mobility in the Languedoc and Garonne areas, at sites dated to the Middle Neolithic (c. 4500–3500 cal bc). Strontium isotope analysis of tooth enamel and bone are a useful geochemical tracer to investigate the origin and residential mobility of ancient people. Strontium isotope ratios (87Sr/ 86Sr) of material from five sites located in two geographic areas were measured. Results obtained do not support our hypothesis of mobility for a number of individuals considered as outsiders in a previous study. On the other hand, the difference recorded between teeth and bone suggests mobility of other individuals between childhood and the last years of adult life. This preliminary study highlights the importance of combining multi-isotope analysis to discuss human subsistence economy and mobility.

A possible case of mycosis in a post-classical burial from La Selvicciola (Italy)

An examination of an adult male buried from the post-classical necropolis of La Selvicciola (Viterbo, Latium, Italy; 4th-6th centuries AD) revealed a series of skeletal lesions. The lesions, both proliferative and lytic, ranging in size from small (around 0.01 mm) to extensive (up to 16.00 mm) pits, occurred at multiple sites. A holistic approach assessed lesion type, frequency and location in a differential diagnosis, which included myeloma, metastatic carcinoma, tuberculosis, leukemia, osteomyelitis, and mycoses. It was concluded that a mycosis, specifically Cryptococcosis, was the most likely cause of these lesions. Both macroscopic analyses and X-ray scans support our diagnosis. We also provide a methodological scheme as a model for examining unknown lesion patterns.

Virtual Anthropology and its Application in Cultural Heritage Studies

ABSTRACT The remains that typically compose the human fossil record often bear cracks, damage, and deformations. The recent rapid development of ‘virtual anthropology’ has provided innovative tools to manage, study, and preserve cultural and natural heritage. Such tools include computerized tomography (CT), laser scanning, photogrammetry, 3D imaging, and rapid prototyping. These approaches can contribute to any archaeological context from the discovery of artefacts to research, preservation, and dissemination. 3D imaging techniques can substitute physical intervention with a virtual protocol to restore the original shape of a fossil specimen. In a similar way, digital morphological information can be recovered using data preserved even on a fragment through the use of 3D comparative samples. Here we present an extended and updated review of the most innovative protocols in virtual anthropology, also applicable in other fields such as natural history and cultural heritage studies, through the description of recent cast studies.

Dietary resilience among hunter-gatherers of Tierra del Fuego: Isotopic evidence in a diachronic perspective

The native groups of Patagonia have relied on a hunter-gatherer economy well after the first Europeans and North Americans reached this part of the world. The large exploitation of marine mammals (i.e., seals) by such allochthonous groups has had a strong impact on the local ecology in a way that might have forced the natives to adjust their subsistence strategies. Similarly, the introduction of new foods might have changed local diet. These are the premises of our isotopic-based analysis. There is a large set of paleonutritional investigations through isotopic analysis on Fuegians groups, however a systematic exploration of food practices across time in relation to possible pre- and post-contact changes is still lacking. In this paper we investigate dietary variation in hunter-gatherer groups of Tierra del Fuego in a diachronic perspective, through measuring the isotopic ratio of carbon (∂13C) and nitrogen (∂15N) in the bone collagen of human and a selection of terrestrial and marine animal samples. The data obtained reveal an unexpected isotopic uniformity across prehistoric and recent groups, with little variation in both carbon and nitrogen mean values, which we interpret as the possible evidence of resilience among these groups and persistence of subsistence strategies, allowing inferences on the dramatic contraction (and extinction) of Fuegian populations.

Evidence of milk consumption in Northern and Southern Middle Bronze Age Italy through a metaproteomic analysis of human dental calculus

• Detecting any difference in the animal species raised Shotgun metaproteomics consists of the proteolytic digestion of proteins belonging to a mixture extremely increased in complexity. We applied a GASP (Gel-Aided Sample Preparation) protocol, modified for ancient mineralized samples [7]. Extractions were performed at BioArCh, Department of Archaeology at the University of York. Extracted peptides were analysed using liquid chromatography tandem mass spectrometry (QExactive) at the Mass Spectrometry laboratory of the Target Discovery Institute at the University of Oxford. Spectra were searched using Mascot (Matrix ScienceTM) against Uniprot database. Dental calculus is used as a powerful tool in bioarchaeology to detect direct evidence of the health status, the diet and possibly the occupation of an individual and/or of a population[4][5][6]. We performed a shotgun metaproteomic analysis of 9 samples of dental calculus; 6 samples from Bovolone, and 3 from Sant’Abbondio. Only supragingival calculus has been sampled, predominately from premolars and molars, and collected in 2.0 mL tubes. We used between 1.9 and 26.7 mg of dental calculus for protein extraction.