Mary Louise Robbins

Inhibition of influenza virus multiplication in tissue culture by structural analogs of amino acids.

Abstract Several amino acid analogs were tested for inhibition of influenza virus in roller tube cultures of mechanically dispersed pooled chick embryo lungs. Norleucine inhibited the growth of influenza A and B viruses in tissue culture. This inhibition was reversed competitively by methionine, and resembled inhibition by methoxinine and ethionine. β-Phenylserine inhibited the growth of the influenza A and B viruses in embryonic chick lung tissue culture. Inhibition was reversed by phenylalanine, acyl phenylalanines, phenylalanine dipeptides, phenylpyruvic acid, and pyridoxal phosphate. Antagonism between phenylalanine and β-phenylserine was of a competitive nature. Canavanine flavianate inhibited the growth of influenza A and B viruses in embryonic chick lung tissue culture. Inhibition was competitively reversed by l -arginine. Canavanine was a mild inhibitor, while flavianic acid acted a virucide. Canavanine flavianate apparently combined these two characteristics into a chemotherapeutic agent superior to either canavanine or flavianic acid alone. Allylglycine and S -ethylcysteine inhibited the growth of the influenza A virus in embryonic chick lung tissue culture. Inhibition by the two compounds was reversed by cysteine and cystine. In addition, the effect of S -ethylcysteine was reversed by other sulfur-containing metabolites. Reversal of virus inhibition by a metabolite was accompanied by a parallel reversal of toxicity of the inhibitor to the tissue. None of the compounds prolonged significantly the life of mice infected with the virus.

Action of Colicines on Shigella boydii and on their Rough and Mucoid Variants

Summary In 58 experiments involving comparisons of 6 smooth parent cultures of various Sh. boydii serotypes and their corresponding non-S variants, the variants were significantly more sensitive to colicine action than the smooth parent strains. Reversal of the culture from the non-S to the S form was accomplished by use of kinetin, and the “transformed” cultures reflected, in part, the colicine spectrum of the original S parent culture and in part the spectrum of the non-S variants from which the “transformed” S cultures were immediately derived.

Inhibitory activity of benzoyl hydrazides and hydrazine on the growth of influenza virus in chick embryo lung tissue culture

Hydrazin, wie auch verschiedene Benzoylhydrazide, hemmen das Wachstum des Influenzavirus in Kulturen embryonalen Hühnerlungengewebes. Anthranilsäurehydrazid konnte die Virusvermehrung bis 6 h nach Viruszugabe hemmen.

Antiviral Activity of 3,5 Diiodo-4-Hydroxybenzenesulf onamide in Chick Embryo Lung Tissue Culture.

Summary 3,5 diiodo-4-hydroxybenzene-sulfonamide was found to inhibit the growth of the influenza virus in chick embryo lung tissue culture. The compound appeared to exert a selective direct effect upon the extracellular virus particle.