Mary Nadijcka

Separation of dead and live mouse spermatozoa

Abstract A modified glass bead column technique is described for separating live from dead mouse spermatozoa. The technique allows one to separate either epididymal or vasa deferentia cells into three fractions: broken spermatozoa and cellular debris; predominantly live spermatozoa; predominantly dead spermatozoa and contaminant cells. This separation method can facilitate those studies which require the initial use of live populations of spermatozoa.