Maryam Abimbola Mikail

Antioxidant activity and phenolic profile of various morphological parts of underutilised Baccaurea angulata fruit.

Baccaurea angulata is an underutilised tropical fruit of Borneo Island of Malaysia. The effect of solvents was examined on yield, total phenolic (TPC), total flavonoids (TFC), total carotene content (TCC), free radical scavenging activities and lipid peroxidation inhibition activities. The results indicated that the pulp (edible portion) had the highest yield, while methanol extracts were significantly (p < 0.01) found to contain higher TPC, TFC and TCC than phosphate buffered saline (PBS) extracts for all the fruits parts. The methanol extracts also showed remarkable antiradical activity and significant lipid peroxidation inhibition activities, with their IC50 results highly comparable to that of commercial blueberry. The variations in the results among the extracts suggest different interactions, such as negative or antagonistic (interference), additive and synergistic effect interactions. The study indicated that B. angulata like other underutilised tropical fruits contained remarkable primary antioxidants. Thus, the fruit has the potential to be sources of antioxidant components.

Physicochemical and antioxidant characteristics of Baccaurea angulata fruit juice extract

The purpose of this study was to explore the physicochemical and antioxidant characteristics of Baccaurea angulata fruit juice extract. Freeze-dried whole fruit (FDWF), freeze-dried berry (FDB), and freeze-dried skin (FDS) of B. angulata were analyzed for total phenolic, total flavonoid, total anthocyanin, and antioxidant activities. FDS recorded the highest moisture and ash content, protein, total fat, and water activity, compared to FDWF and FDB. FDS also contained the highest total phenolic, total flavonoid, and total anthocyanin, while FDWF recorded the highest in scavenging xanthine oxidase (35.9%) and ferric reducing activity (44.9 μM TE/g). FDS, however, showed the highest DPPH (102.66 mg AA/100 g) and TEAC (847.46 mg TE/100 g) values. DPPH, TEAC and FRAP were strongly correlated with total phenol (r = 0.979; 0.948; 0.997) and total flavonoid (r = 0.987; 0.960; 0.992). Total anthocyanin had no correlation with DPPH and TEAC, but moderately with FRAP (r = 0.734). Physicochemical and antioxidant characteristics of B. angulata may indicate that this fruit may impart health benefits when consumed and can be suggested as a good source for nutraceutical beverages. Keywords: Antioxidant properties, Baccaurea angulata , freeze-dried fruit, nutritional composition, physicochemical. African Journal of Biotechnology Vol. 12(34), pp. 5333-5338

Baccaurea angulata fruit juice ameliorates altered hematological and biochemical biomarkers in diet-induced hypercholesterolemic rabbits

Hypercholesterolemia is an important risk factor linked to the alteration of blood hematology and clinical chemistry associated with the development and progression of atherosclerosis. Previous studies have demonstrated the safety and potential health benefits of Baccaurea angulata (BA) fruit. We hypothesized that the oral administration of BA fruit juice could ameliorate the alteration in the hematological and biochemical biomarkers of diet-induced hypercholesterolemic rabbits. The aim of this study was to investigate the effects of different doses of BA juice on the hematological and biochemical biomarkers in normo- and hypercholesterolemic rabbits. Thirty-five healthy adult New Zealand White rabbits were assigned to seven different groups for 90days of diet intervention. Four atherogenic groups were fed a 1% cholesterol diet and 0, 0.5, 1.0, and 1.5mL of BA juice per kg of rabbit daily. The other three normal groups were fed a commercial rabbit pellet diet and 0, 0.5, and 1.0mL of BA juice per kg of rabbit daily. Baseline and final blood samples after 90days of repeated administration BA juice were analyzed for hematological parameters while serum, aortic and hepatic lysates were analyzed for lipid profiles and other biochemical biomarkers. The alteration of the hemopoietic system, physiological changes in serum and tissues lipid profiles and other biochemicals resulting from the consumption of a high-cholesterol diet were significantly (P<.05) ameliorated by the administration of BA juice. Improvements of the biomarkers in rabbits were dose-dependent, markedly enhanced at the highest dose of juice (1.5mL/kg/day). The results suggest potential health benefits of the antioxidant-rich BA fruit juice against hypercholesterolemia-associated hematological and biochemical alterations in the rabbit.

Antimicrobial Effect of Baccaurea angulata Fruit Extracts against Human Pathogenic Microorganisms

The application and research for drugs and food supplements derived from plants extracts have increased in recent years. Plants extract and their constituents are recognized to be safe, either because of their traditional use without any documented detrimental impact or because of dedicated toxicological studies. The potential of higher plants as a source for new drugs is largely unexplored. Although hundreds of plant species have been tested for antimicrobial properties, the vast majority of medicinal plants have not been adequately evaluated. And an increasing number of pathogens are left off treatment, due to emergent resistance strains. Thus, a systematic investigation was undertaken to screen for antibacterial activity from Baccaurea angulata (BA). The Baccaurea angulata belongs to the family of Euphorbiaceae. Plant that belongs to the family is used as food as well as treatment of infectious diseases such as diarrhea, skin infections and gonorrhoea. The anti-microbial activity of the BA fruit extracts havee revealed different antimicrobial properties, that are various between three parts (whole fruit, fruit skin, and berry), three solvents (methanol, ethanol and aqueous), different methods (agar well diffusion, and microdilution method) and differently listed pathogens (Streptococcus pneumonia, Staphylococcus epidermidis, Klebsiella pneumonia and Pseudomonas aeruginosa). The highest observed antimicrobial activity was in ethanol extract of fruit skin using agar well diffusion against S. pneumonia. Among tested Gram negative bacteria K. pneumoniae was the most susceptible bacterium which showed the highest bactericidal and bacteriostatic activity using microdilution method.