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Dive into the research topics where Marylène Chollet-Krugler is active.

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Featured researches published by Marylène Chollet-Krugler.


Journal of Chromatography B | 2009

Multiple dual-mode centrifugal partition chromatography as an efficient method for the purification of a mycosporine from a crude methanolic extract of Lichina pygmaea

Catherine Roullier; Marylène Chollet-Krugler; Aurélie Bernard; Joël Boustie

Centrifugal partition chromatography method was applied to the separation and purification of a crude methanolic extract of a cyanobacterial lichen, Lichina pygmaea. A multiple dual-mode was used to separate two compounds of interest, namely mycosporine-serinol and a glutamic acid derivative. These compounds are described here for the first time in a lichen. Their structures were identified by UV, IR, ESI-MS, (1)H NMR, (13)C NMR, and 2D NMR.


Bioorganic & Medicinal Chemistry Letters | 2010

A novel aryl-hydrazide from the marine lichen Lichina pygmaea: isolation, synthesis of derivatives, and cytotoxicity assays.

Catherine Roullier; Marylène Chollet-Krugler; Pierre van de Weghe; Françoise Lohézic-Le Dévéhat; Joël Boustie

A new aryl-hydrazide l-glutamic acid derivative, pygmeine (3), was isolated from a methanolic extract of Lichina pygmaea, a marine lichen. Synthetic derivatives obtained via a two-step coupling of l-glutamic acid with phenylhydrazine moieties were useful to elucidate the structure of 3 and to carry out biological assays. Thus, the cytotoxicity of the ortho-, meta-, and para-hydroxyl isomers along with their respective benzyl intermediates, and a natural methoxylated analog, were evaluated on murine and human melanoma cells (B16, A375). The para-hydroxyl isomer 6 was found to be the most active (IC(50)=1.6 microM) on B16 cells.


Phytochemistry | 2011

Characterization and identification of mycosporines-like compounds in cyanolichens. Isolation of mycosporine hydroxyglutamicol from Nephroma laevigatum Ach.

Catherine Roullier; Marylène Chollet-Krugler; Eva-Maria Pferschy-Wenzig; Anne Maillard; Gerald N. Rechberger; Béatrice Legouin-Gargadennec; Rudolf Bauer; Joël Boustie

Mycosporine-like compounds, comprising mycosporines and mycosporine-like amino acids (MAAs) are UV protecting secondary metabolites described in organisms such as fungi, algae, cyanobacteria or animals. Lichens however, were only poorly investigated for such constituents so far. Here, a method for the characterization of mycosporines and MAAs in purified aqueous extracts, involving HPTLC coupled to spectrophotodensitometry, HPLC-DAD-MS(n) and UPLC-HRMS analysis, is described. This optimized protocol was validated on three algae and one cyanolichen containing known MAAs and mycosporines, and then applied to 18 cyanolichen species. Analyses revealed the presence of five already described mycosporine-like compounds in the investigated species, including mycosporine serinol in Lichina and Peltigera species and mycosporine glutamicol in Degelia plumbea. Apart from that, eight unknown mycosporine-like compounds were detected and tentatively characterized on the basis of their DAD spectra and their MS(n) and HRMS data: two in the alga Porphyra dioica and six in cyanolichen species belonging to the genera Degelia, Nephroma and Stereocaulon. From Nephroma laevigatum, the mycosporine hydroxyglutamicol was preparatively isolated and identified through HRMS, 1D and 2D NMR spectroscopic data. The optimized analytical protocol allowed the characterization of mycosporine-like compounds in small amounts of material and confirmed the potential of cyanolichens as a source of mycosporine compounds. It should also be applicable to investigate lichen species with green algae photobionts for mycosporine-like compounds.


Phytomedicine | 2016

Lichen-derived compounds show potential for central nervous system therapeutics

R. Gajendra Reddy; Lenin Veeraval; Swati Maitra; Marylène Chollet-Krugler; Sophie Tomasi; Françoise Lohézic-Le Dévéhat; Joël Boustie; Sumana Chakravarty

BACKGROUND Natural products from lichens are widely investigated for their biological properties, yet their potential as central nervous system (CNS) therapeutic agents is less explored. PURPOSE The present study investigated the neuroactive properties of selected lichen compounds (atranorin, perlatolic acid, physodic acid and usnic acid), for their neurotrophic, neurogenic and acetylcholine esterase (AChE) activities. METHODS Neurotrophic activity (neurite outgrowth) was determined using murine neuroblastoma Neuro2A cells. A MTT assay was performed to assess the cytotoxicity of compounds at optimum neurotrophic activity. Neuro2A cells treated with neurotrophic lichen compounds were used for RT-PCR to evaluate the induction of genes that code for the neurotrophic markers BDNF and NGF. Immunoblotting was used to assess acetyl H3 and H4 levels, the epigenetic markers associated with neurotrophic and/or neurogenic activity. The neurogenic property of the compounds was determined using murine hippocampal primary cultures. AChE inhibition activity was performed using a modified Ellmans esterase method. RESULTS Lichen compounds atranorin, perlatolic acid, physodic acid and (+)-usnic acid showed neurotrophic activity in a preliminary cell-based screening based on Neuro2A neurite outgrowth. Except for usnic acid, no cytotoxic effects were observed for the two depsides (atranorin and perlatolic acid) and the alkyl depsidone (physodic acid). Perlatolic acid appears to be promising, as it also exhibited AChE inhibition activity and potent proneurogenic activity. The neurotrophic lichen compounds (atranorin, perlatolic acid, physodic acid) modulated the gene expression of BDNF and NGF. In addition, perlatolic acid showed increased protein levels of acetyl H3 and H4 in Neuro2A cells. CONCLUSION These lichen depsides and depsidones showed neuroactive properties in vitro (Neuro2A cells) and ex vivo (primary neural stem or progenitor cells), suggesting their potential to treat CNS disorders.


Bioorganic & Medicinal Chemistry | 2016

Design, synthesis and biological evaluation of potential antibacterial butyrolactones

Alaa Sweidan; Marylène Chollet-Krugler; Pierre van de Weghe; Ali Chokr; Sophie Tomasi; Martine Bonnaure-Mallet; Latifa Bousarghin

Novel butyrolactone analogues were designed and synthesized based on the known lichen antibacterial compounds, lichesterinic acids (B-10 and B-11), by substituting different functional groups on the butyrolactone ring trying to enhance its activity. All synthesized butyrolactone analogues were evaluated for their in vitro antibacterial activity against Streptococcus gordonii. Among the derivatives, B-12 and B-13 had the lowest MIC of 9.38μg/mL where they have shown to be stronger bactericidals, by 2-3 times, than the reference antibiotic, doxycycline. These two compounds were then checked for their cytotoxicity against human gingival epithelial cell lines, Ca9-22, and macrophages, THP-1, by MTT and LDH assays which confirmed their safety against the tested cell lines. A preliminary study of the structure-activity relationships unveiled that the functional groups at the C4 position had an important influence on the antibacterial activity. An optimum length of the alkyl chain at the C5 position registered the best antibacterial inhibitory activity however as its length increased the bactericidal effect increased as well. This efficiency was attained by a carboxyl group substitution at the C4 position indicating the important dual role contributed by these two substituents which might be involved in their mechanism of action.


Dalton Transactions | 2008

Preparation and characterization of copper(II) and nickel(II) complexes of a new chiral salen ligand derived from (+)-usnic acid.

Marylène Chollet-Krugler; Sophie Tomasi; Philippe Uriac; Loïc Toupet; Pierre van de Weghe

Chiral copper(II) and nickel(II) complexes were obtained after reaction of diacetate salts with a new chiral salen ligand derived from (+)-usnic acid.


Sulfur Letters | 2003

On the preparation of 3-(5-Formyl-3-thioxo-3H-[1,2]dithiol-4-yl)-propionic acid

Marylène Chollet-Krugler; Jean-Louis Burgot

3-(5-Formyl-3-thioxo-3H-[1,2]dithiol-4-yl)propionic acid has been obtained from 3-[5-(hydroxyiminomethyl)-3-thioxo-3H-[1,2]dithiol-4-yl]propionic acid under standard conditions. Conversely, for the hydroxyimino derivative the general procedure failed and so it has been prepared by the action, in a basic medium, of isoamyl nitrite on 3-(5-methyl-3-thioxo-3H-[1,2]dithiol-4-yl)propionic acid ethyl ester.


Fitoterapia | 2017

Antibacterial activities of natural lichen compounds against Streptococcus gordonii and Porphyromonas gingivalis

Alaa Sweidan; Marylène Chollet-Krugler; Aurélie Sauvager; Pierre van de Weghe; Ali Chokr; Martine Bonnaure-Mallet; Sophie Tomasi; Latifa Bousarghin

The oral bacteria not only infect the mouth and reside there, but also travel through the blood and reach distant body organs. If left untreated, the dental biofilm that can cause destructive inflammation in the oral cavity may result in serious medical complications. In dental biofilm, Streptococcus gordonii, a primary oral colonizer, constitutes the platform on which late pathogenic colonizers like Porphyromonas gingivalis, the causative agent of periodontal diseases, will bind. The aim of this study was to determine the antibacterial activity of eleven natural lichen compounds belonging to different chemical families and spanning from linear into cyclic and aromatic structures to uncover new antibiotics which can fight against the oral bacteria. The compounds were screened by broth microdilution assay. Three compounds were shown to have promising antibacterial activities where the depsidone core with certain functional groups constituted the best compound, psoromic acid, with the lowest MICs=11.72 and 5.86μg/mL against S. gordonii and P. gingivalis, respectively. The compounds screened had promising antibacterial activity which might be attributed to some important functional groups as discussed in our study. The best compounds did not induce the death of gingival epithelial carcinoma cells (Ca9-22). These results introduce new compounds having potent antibacterial activities against oral pathogens causing serious medical complications.


Phosphorus Sulfur and Silicon and The Related Elements | 2005

Synthesis and Physicochemical Properties of Methyl 3-Mercapto Arylpropene Dithioates

Marie-Laurence Abasq; Jean-Louis Burgot; Marylène Chollet-Krugler; Patricia Courtel; Béatrice Legouin

1,2-dithiole-3-thiones and derivatives are endowed with several promising pharmacological properties.1 We have shown that the reduction of 3-(methylsulfanyl)-5-(phenyl)-1,2-dithiolium ion 1a1 by NaBH4 is an efficient way to prepare the corresponding reduction product: methyl3-mercapto-3-phenylpropene dithioate 2a1. We present here the generalization of this reaction to other 3-(methylsulfanyl)-1,2-dithiolium ions 1 to give methyl-3-mercapto-3-phenylpropene dithioates 2. Compounds 2a1 and 2a2 have been previously described according to other synthetic routes.3,4


Natural Product Reports | 2013

UV-protectant metabolites from lichens and their symbiotic partners

Khanh-Hung Nguyen; Marylène Chollet-Krugler; Nicolas Gouault; Sophie Tomasi

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Sophie Tomasi

Centre national de la recherche scientifique

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Marie-Dominique Galibert-Anne

Centre national de la recherche scientifique

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Thuc Huy Duong

Chulalongkorn University

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