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Dive into the research topics where Masae Kaneko is active.

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Featured researches published by Masae Kaneko.


Biochimica et Biophysica Acta | 1996

Molecular cloning and sequencing of a cDNA encoding dihydropyrimidinase from the rat liver

Koichi Matsuda; Shigeko Fujimoto Sakata; Masae Kaneko; Naoki Hamajima; Masaru Nonaka; Makoto Sasaki; Nanaya Tamaki

A cDNA encoding dihydropyrimidinase has been isolated from a rat cDNA library. The N-terminal and an internal amino acid sequences were determined, and PCR primers were designed based on these sequences. Using a cDNA fragment amplified by RT-PCR with these primers, three cDNA clones were isolated from a rat liver library. The clone with the longest insert of 2129 bp contained a 1557 bp open reading frame encoding a polypeptide of 519 residues with a molecular mass of 56,832 Da.


British Journal of Nutrition | 1995

Analysis of cyclic feed intake in rats fed on a zinc-deficient diet and the level of dihydropyrimidinase (EC 3.5.2.2).

Nanaya Tamaki; Shigeko Fujimoto-Sakata; Mariko Kikugawa; Masae Kaneko; Satomi Onosaka; Tatsuya Takagi

The body weight and feed intake of rats fed on a Zn-deficient diet for 28 d were reduced compared with those of control rats. The feed intakes of the Zn-deficient and control groups during the period were 10.2 (SE 0.3) and 15.7 (SE 0.2) g/d respectively. Cyclic variations in feed intake and body-weight changes were found in analysis not only of all the data for five rats but also that in each individual rat. Cosinor analysis revealed that the cyclical period of both the feed intake and body-weight change in the Zn-deficient rats was 3.5 (SE 0.1) d. The mesor and amplitude value of the feed intake in the Zn-deficient rats was 10.1 (SE 0.4) g/d and 3.5 (SE 0.5) g/d respectively, and that of body-weight change was 1.4 (SE 0.1) g/d and 7.9 (SE 1.3) g/d respectively. Among pyrimidine-catabolizing enzymes, dihydropyrimidinase (EC 3.5.2.2) activity showed significant retardation in the Zn-deficient rat liver with decrease of the enzyme protein. The ratio of apo-form to holo-form dihydropyrimidinase in the liver was not affected by the Zn-deficient diet.


Biochimica et Biophysica Acta | 1992

Inhibition of d-3-aminoisobutyrate-pyruvate aminotransferase by 5-fluorouracil and α-fluoro-β-alanine

Masae Kaneko; Yasuhide Kontani; Mariko Kikugawa; Nanaya Tamaki

Among pyrimidine derivatives, we found that 5-fluorouracil;potently inhibited purified rat liver d -3-aminoisobutyrate-pyruvate aminotransferase, whereas 5-fluorouridine did so to a much lesser extent. 5-Fluorouracil acted as a competitive inhibitor against β-alanine with a K i of 56 μM, and was uncompetitive against pyruvic acid, with a K i of 73 μM. α-Fluoro-β-alanine, a metabolite of 5-fluorouracil, was also a competitive inhibitor with respect to β-alanine with a K i of 8.0 mM. 5-Fluorouracil acted also as a competitive inhibitor of 4-aminobutyrate aminotransferase with respect to β-alanine with a K i value of 1.9 mM, and was uncompetitive against 2-oxoglutaric acid, with a K i of 1.8 mM.


Biochimica et Biophysica Acta | 1990

Evaluation of interconversion between (R)- and (S)-enantiomers of β-aminoisobutyrate

Nanaya Tamaki; Masae Kaneko; Mariko Kikugawa; Shigeko Fujimoto

Abstract The conversion of (R)- to (S)-β-aminoisobutyrate was observed in the presence of d -3-aminoisobutyrate-pyruvate aminotransferase, aminobutyrate aminotransferase, pyruvate and l -glutamate. The reverse reaction was also found in the presence of 2-oxoglutarate and l -alanine. Neither d -3-aminoisobutyrate-pyruvate aminotransferase nor aminobutyrate aminotransferase revealed a racemase activity of the enantiomorphs.


FEBS Journal | 1994

Purification, characterization and inhibition of dihydropyrimidinase from rat liver

Mariko Kikugawa; Masae Kaneko; Shigeko Fujimoto-Sakata; Mitsuko Maeda; Koichi Kawasaki; Tatsuya Takagi; Nanaya Tamaki


Biochimica et Biophysica Acta | 1993

Identity of D-3-aminoisobutyrate-pyruvate aminotransferase with alanine-glyoxylate aminotransferase 2

Yasuhide Kontani; Masae Kaneko; Mariko Kikugawa; Shigeko Fujimoto; Nanaya Tamaki


FEBS Journal | 1990

Purification, characterization and inhibition of D-3-aminoisobutyrate aminotransferase from the rat liver

Nanaya Tamaki; Masae Kaneko; Chizuru Mizota; Mariko Kikugawa; Sigeko Fujimoto


Journal of Nutritional Science and Vitaminology | 1991

Effect of Vitamin B2 Deficiency on Rat Liver Dihydropyrimidine Dehydrogenase Activity

Shigeko Fujimoto; Koichi Matsuda; Mariko Kikugawa; Masae Kaneko; Nanaya Tamaki


Journal of Nutritional Science and Vitaminology | 1998

Cloning of Rat Dihydropyrimidine Dehydrogenase and Correlation of Its mRNA Increase in the Rat Liver with Age.

Masahiro Kimura; Shigeko Fujimoto Sakata; Yasuhiro Matoba; Koichi Matsuda; Yasuhide Kontani; Masae Kaneko; Nanaya Tamaki


Journal of Nutritional Science and Vitaminology | 1997

Cosinor Analysis of Feed Intake Cycle of Rats Fed a Zinc-Deficient Diet and the Effect of Zinc Supplementation

Kazuhiro Aiba; Masahiro Kimura; Shigeko Fujimoto Sakata; Koichi Matsuda; Masae Kaneko; Satomi Onosaka; Yumiko Yamaoka; Nanaya Tamaki

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