Masahiro Shimoda
Tokyo Institute of Technology
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Publication
Featured researches published by Masahiro Shimoda.
Journal of Molecular Catalysis | 1991
Masahiro Shimoda; Shuzo Nemoto; Ichiro Okura
Abstract Alcohol formation was studied by the hydroxylation of alkanes with Methylosinus trichosporium (OB3b). When M. trichosporium was treated with cyclopropane, accumulation of primary alcohol (methanol, ethanol, 1-propanol, 1-butanol) was observed from the respective lower alkane (methane, ethane, propane, n-butane). It was found that cyclopropane was a selective inhibitor for the alcohol dehydrogenase contained in the same bacterium, and that alcohol oxidation with this enzyme was inhibited. The inhibition mechanism is also discussed.
Journal of Molecular Catalysis | 1994
Yuji Seki; Masahiro Shimoda; Daisuke Sugimori; Ichiro Okura
Abstract Oxidation of halogenated allyl compounds was carried out with Methylosinus trichosporium and gave the relevant 1,2-epoxy-3-halopropane. Stereoselectivity was found to depend strongly on the type and size of the functional group at the allyl position.
Journal of Molecular Catalysis | 1992
Kenji Yamada; Masahiro Shimoda; Ichiro Okura
Abstract Methanol dehydrogenase from Methylosinus trichosporium (OB3b) was purified and characterized. The substrates of this enzyme were non-branched primary alcohols. Secondary alcohols were hardly oxidized by the enzyme. The enzyme was deactivated by cyclopropanol because of complex formation.
Journal of Molecular Catalysis | 1993
Masahiro Shimoda; Yuji Seki; Ichiro Okura
Abstract Halogenated propanes (1-chloropropane, 1-bromopropane, 2-chloropropane, and 2-bromopropane) were oxidized with Methylosinus trichosporium (OB3b). 1-Propanol, 1-halogenated-2-propanol, and 1-halogenated-3-propanol were produced from 1-halogenated propane, and 2-halogenated-1-propanol and acetone were produced from 2-halogenated propane, showing that all the carbon atoms of the halogenated propanes were attacked by activated oxygen. The optical purity of halohydrin obtained from halogenated propane with M. trichosporium was independent of halogen type.
Journal of Molecular Catalysis | 1992
Masahiro Shimoda; Ichiro Okura
Abstract Propane and deuterated propane hydroxylation were carried out with Methylosinus trichosporium (OB3b). From the hydroxylation rate and the product selectivity observed, a reaction mechanism for propane hydroxylation is discussed.
Journal of Molecular Catalysis | 1991
Tsutomu Katayama; Masahiro Shimoda; Ichiro Okura
Abstract NADP-dependent alcohol dehydrogenase was partially purified from Methylosinus trichosporium (OB3b). In this bacterium there were two kinds of alcohol dehydrogenases, PQQ-dependent and NADP-dependent dehydrogenases. For NADP-dependent alcohol dehydrogenase, secondary alcohols were suitable substrates.
Journal of The Chemical Society, Chemical Communications | 1990
Masahiro Shimoda; Ichiro Okura
Methanol accumulation was observed by methane hydroxylation when Methylosinus trichosporium(OB3b) was treated with cyclopropane.
Nippon Kagaku Kaishi | 1998
Masami Suzuki; Fumihiko Hasumi; Masahiro Shimoda; Kazuhiro Hamamatsu; Akihiko Uchiyama; Ichiro Okura
With the objective to prevent moth-eating damage caused by insects, the system containing bakers yeast adsorbed by a water-absorbing polymer, Diawet US60, was prepared. The moth-eating damage caused by Attagenus unicolor japonicus was evaluated by use of a woolen cloth (2 cm2, 40 mg) with or without the system in 1 L glass vessel at 25 °C for 7 days. The moth-eating damage rates with and without the system were 3.3% and 54%, respectively, and the death rate of insect reached 100% with the system due to the respiration of yeasts.
Journal of Molecular Catalysis | 1991
Masahiro Shimoda; Ichiro Okura
Journal of Molecular Catalysis | 1993
Masahiro Shimoda; Yuji Seki; Ichiro Okura