Masahito Tsuchiya

Growth factor for cardiac hypertrophy

Cardiac size can be regulated by the balance in activity between cardiac growth factors and inhibiting factors, chalones. This study was undertaken to verify the role of the cardiac growth factor and its purification from hypertrophied hearts. For this propose the hypertrophied hearts of renovascular hypertensive rats were used. The purification was made by using an isoelectric focusing chromatography and the HPLC method. We examined the cardiac growth effect of the isolated fractions with cultured chicken embryonic cardiac myocytes. Simultaneously, the influence of these fractions on the cardiac cell cycle was examined by DNA analysis with the flow cytometric method. If the hearts were overloaded due to hypertension, the growth of the cardiac size could be induced by increased the level of five proteins with different molecular weight and with an isoelectric point of 8.3. The significant growth activities were observed at these five proteins compared to the absence of the fractions. For the appearance of these growth effect, it is necessary that the structure of the protein is there fundamentally as a form with a molecular weight of 27 k dalton. After addition of these isolated fractions, BrdU content is S and G2 phases by flow cytometry was increased. This change indicates that the cardiac myocytes are stimulated in form DNA synthesis.

Molecular biologic Changes of Adenine Nucleotide Translocator in J-2-N Cardiomyopathic Hamsters

The adenine nucleotide translocator (ANT) is an integral protein present in the inner mitochondrial membrane that performs the exchange of cytoplasmic and intramitochondrial ADP and ATP. The ANT content of the myocardium was studied in J-2-N cardiomyopathic hamsters. The ANT content was significantly decreased in J-2-N hamsters. By molecular biologic analysis, hamster ANT cDNA of the Tl isoenzyme was cloned by the plaque hybridization method. The ANT cDNA hybridized specifically with ANT mRNA, so RNA dot-blot hybridization was performed. The highest ANT mRNA level was observed in control hamsters followed by J-2-N hamsters with mild myocardial damage, J-2-N hamsters with severe myocardial damage, and Bio 14.6 cardiomyopathic hamsters. These results suggest that a decreased ANT content may contribute to the pathogenesis of cardiomyopathy and that a decrease in ANT mRNA levels may explain the abnormalities of ANT in J-2-N cardiomyopathic hamsters.

A Case of Terminal Ileum Lipoma Treated by Endoscopic Polypectomy

Abstract: This study reports on the case of a 71‐year‐old man who complained of repeated episodes of right lower abdominal pain. A barium enema and colonoscopy revealed a 20 times 20 times 15 mm smooth‐surfaced polypoid tumor (Yamada type III) located in the terminal ileum.