Masami Takahashi

Inhibition of poliovirus by effect of a methylthiopyrimidine derivative.

Summary Ethyl-2-methylthio-4-methyl-5-pyrimidinecarboxylate (S-7) inhibited the cytopathic effect and multiplication of poliovirus in primary monkey kidney cultures but not that of ECHO 1, 5, 13, 22, 23, Coxsackie A7, A9, B3, or B5. It did not inhibit cell multiplication under these conditions. Passage of poliovirus type 2 in the presence of S-7 gave rise to S-7-resistant and S-7-dependent strains. There was no cross resistance between S-7 and guanidine or HBB.

Effects of moxibustion on the enhancement of serum antibody in rabbit against Staphylococcus aureus

The titer and activity of antibody in rabbits immunized with heat-killed vaccine were assessed with and without moxibustion treatment. Enzyme-linked immunosorbent assay (ELISA) was applied for the detection of immunoglobulins to Smith strain of Staphylococcus aureus. Positive IgM titer of more than 0.4 were observed with this assay against the moxibustion group (P < 0.05). The titer of IgG antibody also increased; however, there was no significant difference between the moxibustion group and the control group. The ELISA inhibition test showed significantly higher protective activity of the sera in the moxibustion group at the 9th week after the first immunization (P < 0.05).

Mouse Virulence of a Strain of Klebsiella ozaenea and Its Variants in Relation to Colonial Morphologies in Soft-agar

ganisms to mouse virulence was investigated by the application of our method described in a previous contribution (7) using a strain of K. pneumoniae and its variants to elucidate the pathogenicity of this species of bacteria. This paper is concerned with these investigations. Strain KO-2 was isolated from bronchial exudate of a patient with acute pneumoniae. On the basis of the biological and serological properties (1, 2), the strain was identified as K. ozaenea. Colonies of the strain grown on a blood agar medium were extremely mucoid. During frequent subcultures on the medium, medium size

[Kidney lodgment of staphylococcus: its relationship to the growth patterns of S. epidermidis and S. aureus in serum-soft agar].

臨床材料よりえた表皮ブドウ球菌compact型株および対応するdiffuse型株,108 colony forming unit (C.F.U.)をマウス尾静脈より接種したところ,5株中2株のcompact型株に腎定着能が認められた。これら腎は肉眼的には膿瘍形成は認められなかつたが,接種後14日経過するもなお多数の生菌が検出されたのに反し,血液,肝臓,脾臓からは接種3日後には生菌を分離しえなかつた。つぎにこれら2株の接種菌量を変えてマウス尾静脈より接種したところ,1株においては106 C.F.U.により7日以上の腎定着能を認めたが,他の1株は同期間の定着には108 C.F.U.の接種を必要とした。つぎに黄色ブドウ球菌Smith株のdiffuse型株,compact型株を用いたところ,compact型株では接種14日後には腎を除く各臓器から菌が消失したが腎からは108/100mgの菌が検出され,膿瘍形成が認められた。しかしながら,diffuse型株では特異臓器定着は認められず敗血症型の感染の経過をとることが認められ,黄株色においても腎定着はcompact型株の特異性と考えられた。

[HA-and HI-tests for myxoviruses with formalinized erythrocytes].

With formalinized erythrocytes (FRC) from chickens, men and guinea-pigs, hemagglutination and hemagglutination-inhibition test for myxoviruses with Salk pattern method were studied and the results obtained are as follows.1) Spontaneous agglutination of FRC was prevented with 0.05 per cent bovine serum albumin in the suspending medium.2) There was no or little if any differences between HA- or HI-titers obtained with FRC and those with native red cells.3) FRC was available for HI test after the lyophylization.4) Hemadsorption test in HVJ-infected HeLa cells was successfully performed with FRC without substitution of bovine serum albumin.5) HVJ-adsorpting capacity of FRC was identical with that of native cells, but the eluting capacity was decreased in FRC.6) The ABO-antigenicity of human red cells was partially destroyed by the formalinization.