Masanori Shima

Metastin Inhibits Migration and Invasion of Renal Cell Carcinoma with Overexpression of Metastin Receptor

BACKGROUND Metastin, the final peptide of the KiSS-1 gene, has been proposed to suppress cell motility. OBJECTIVE This study investigated whether renal cell carcinoma (RCC) tissue expresses metastin or its receptor, and clarified whether metastin can suppress migration and/or invasion and/or proliferation of RCC cells in vitro. DESIGN, SETTING, AND PARTICIPANTS Twenty-five RCC samples were submitted. Fresh RCC tissues were prepared for real-time RT-PCR, and formalin-fixed and paraffin-embedded tissues blocks were examined by immunohistochemistry. RCC cell lines Caki-1 and ACHN were supplied for cell migration, invasion, and proliferation assays. MEASUREMENTS Real-time RT-PCR was performed by using Taq Man gene expression system. ENVISION system was used in immunohistochemistry. Wound-healing assay and matrigel assays were used to identify migration and invasion abilities of RCC cell lines. Cell Counting Kit-8 was applied to measure the cell proliferation. Cell morphology was examined under a META system. Statistical analysis was performed with SPSS15.0J. RESULTS AND LIMITATIONS In twenty-five RCC samples, the mRNA level of metastin receptor was identified to be significantly higher than non-neoplastic renal cortex by real-time RT-PCR (p=0.011). Immunohistochemical study also detected metastin receptor protein in all RCC tumors. In vitro, this study showed that metastin inhibited migration and invasion of Caki-1 and ACHN cells. In contrast, it had no effects on cell proliferation. Metastin (10 micromol/l) induced excessive formation of focal adhesions and stress fibers in Caki-1 and ACHN cells; this phenomenon was inhibited by pretreating pharmacological Rho-kinase inhibitor (Y-27632) to those cells. CONCLUSION This is the first report regarding overexpression of the metastin receptor hOT7T175 in human RCC. We demonstrate that metastin can inhibit migration and invasion of the RCC cell line, which is regulated by a Rho-kinase inhibitor. Metastin and its receptor are therefore probable targets for suppressing RCC.

OVER-EXPRESSION OF METASTIN RECEPTOR IN RENAL CELL CARCINOMA: METASTIN AND ITS RECEPTOR ARE A POSSIBLE INHIBITOR OF THE INVASION OF RENAL CELL CARCINOMA

245 OVER-EXPRESSION OF METASTIN RECEPTOR IN RENAL CELL CARCINOMA: METASTIN AND ITS RECEPTOR ARE A POSSIBLE INHIBITOR OF THE INVASION OF RENAL CELL CARCINOMA Sunao Shoji*, Xian Yan Tang, Shinobu Umemura, Masanori Shima, Yukio Usui, Yoshihiro Nagata, Toyoaki Uchida, Robert Y Osamura, Toshiro Terachi. Isehara, Kanagawa, Japan. INTRODUCTION AND OBJECTIVE: KiSS-1 is a tumor suppressor gene, which can prevent breast cancer and melanoma KiSS-1 gene encodes a 145-amino acid

Prostate cancer with cyst formation detected by whole body positron emission tomography/computed tomography: A case report

The present study reports a case of prostate adenocarcinoma with cyst formation. A 72-year-old male diagnosed with multiple lung metastases at a local clinic was referred to Tokai University Hachioji Hospital (Tokyo, Japan) for detection of a primary lesion. Whole body positron emission tomography/computed tomography showed strong accumulation of [18F]-fluoro-deoxy-2-glucose (FDG) in the small pelvis, and pelvic magnetic resonance imaging revealed a 60×40-mm cystic lesion, with an irregular thickened wall, behind the prostate. The serum prostate-specific antigen (PSA) level was elevated to 211.99 ng/ml, therefore, the patient underwent a transperineal prostate needle biopsy, and was diagnosed with prostate adenocarcinoma with cyst formation. Androgen deprivation therapy was administered for 8 months following the diagnosis of prostate cancer; consequently, the cyst reduced in size and the serum PSA level decreased to 0.14 ng/ml. To the best of our knowledge, this is the first report of a malignant prostatic cyst detected by FDG-positron emission tomography/computed tomography.

Erratum: Evaluation of renal function after laparoscopic partial nephrectomy with renal scintigraphy using 99mtechnetium- mercaptoacetyltriglycine (International Journal of Urology 13, (1371-1374))

Yasuyuki, K, Yukio, U, Masanori, S, Hoshi, A, Hideshi, M, Hiroyoshi, I and Toshiro, T. Evaluation of renal function after laparoscopic partial nephrectomy with renal scintigraphy using technetium-mercaptoacetyltriglycine. Int. J. Urol. 13: 1371–1374. The publisher would like to draw the readers’ attention to an error in the above article. The authors should read: Yasuyuki Kobayashi, Yukio Usui, Masanori Shima, Hoshi Akio, Hideshi Miyakita, Hiroyoshi Inatsuchi and Toshiro Terachi. The author would like apologize for this error and any inconvenience caused. International Journal of Urology (2007) 14, 179 doi: 10.1111/j.1442-2042.2006.01735.x

Laparoscopic Radical Prostatectomy: Oncological and Functional Results

Between December 1999 and December 2004, 160 patients with organ-confined prostate cancer underwent laparoscopic radical prostatectomy (LRP) by two surgeons with many different assistants. The patients were divided into two groups in order of the date of surgery. Groups I and II consisted of 66 and 94 patients who were operated on between December 1999 and March 2002 and between April 2002 and December 2004, respectively. Group I was subdivided into Group I-a and Group I-b: Group I-a consisted of 36 patients who underwent LRP at Tenri Hospital and Group I-b consisted of 30 patients who underwent LRP at the other institutions in the same period. The patients in Group I-b were evaluated only for operative morbidity and excluded from analysis of oncological outcome because of insufficient pathological data. Mean operative time and mean blood loss including leaked urine in Group II were 291 ± 57.2min (range 145–425min) and 401.0 ± 323.7 g (range 14–1859 g). There was no blood transfusion in Group II and no operative conversion to an open retropubic radical prostatectomy (RRP). Mean postoperative urethral catheter indwelling period and mean postoperative hospital stay were 4 ± 4.8 days (range 3–28) and 8 ± 4.5 days (range 4–25) in Group II, respectively. Positive surgical margin was detected in 18/130 cases (13.8%) for all pathological stages in this series, but 7/105 cases (6.6%) with pT2 and pT0 disease. Positive surgical margin was detected at the apical margin most frequently. To achieve earlier recovery of urinary continence, a ligation of the dorsal vein complex (DVC bunching) was decided against and substituted by closure of DVC stump with a vertical running suture for hemostasis. In the latter group in Group II, 55% and 87% of the patients were almost dry at 1 and 3 months postoperatively, respectively. Eight of 31 patients (25.8%) who had preserved unilateral neurovascular bundle (NVB) regained potency to be able to carry out sexual intercourse between 6 and 15 months postoperatively. Less morbidity, encouraging early oncological results and improved early recovery of urinary continence, are favorable factors for LRP; however, long-term follow-up and consecutive effort to improve the apical section procedure are required for establishment of LRP as a reliable treatment tool for organ-confined prostate cancer.