Masao Yoshizaki

Hydrolysable tannins as chemotaxonomic markers in the Rosaceae

Abstract A HPLC survey of leaves of 80 plants (62 species, 15 hybrids, one variety and two cytotypes) from 18 genera of four subfamilies of Rosaceae, using five oligomeric hydrolysable tannins, five monomeric hydrolysable tannins, and chlorogenic acid as reference compounds, showed that the oligomers can be used as chemotaxonomic markers for the family, viz., sanguiin H-6 and H-11 in the genera Sanguisorba and Rubus , gemin A in Geum , agrimoniin in Agrimonia , Fragaria and Potentilla , and rugosin D in Filipendula . The hydrolysable tannin monomers were widely distributed in the herbaceous and frutescent Rosoideae species, but not in the arborous species of the other subfamilies. Chlorogenic acid was found in almost all of the plants examined.

Scopadulcic acid-A and -B, new diterpenoids with a novel skeleton, from a Paraguayan crude drug “typychá kuratũ” (scoparia dulcis L.)

Abstract Scopadulcic acid-A and -B, diterpenoids with a novel skeleton, have been isolated from the whole plants of Scopari dulcis L., and their structures, including the absolute configuration, were determined based on the 2-D NMR and CD spectral data.

Liquidambin, an ellagitannin from Liquidambar formosana

Abstract A new ellagitannin, named liquidambin, which could be biogenetically closely correlated with casuarinin and pedunculagin, has been isolated from the leaves of Liquidambar formosana . Its structure was determined as 5- O -galloyl-2,3,4,6-di- O -(S)-hexahydroxydiphenoyl- d -glucose. The structural equilibration due to hydration of the aldehyde group of the glucose core in this tannin was shown from its 1 H and 13 C NMR spectra.

A chemotaxonomic study of flavonoids in the leaves of six Trichosanthes species

Abstract The 7-, 3′- and 4′-glucosides of luteolin, the 7-glucoside and 6,8-di- C -glucoside of apigenin were isolated from Trichosanthes kirilowii var. japonica . Kaempferol 3,7-di-rhamnoside and 3-glucoside-7-rhamnoside were identified from T. cucumeroides , kaempferol 3-galactoside and 3-sophoroside were also identified from T. anguina . Quercetin-3-rutinoside was detected from T. multiloba and T. rostrata. T. bracteata afforded luteolin 3′-glucoside and kaempferol 3-rutinoside, and T. kirilowii afforded luteolin 7-, 3′- and 4′-glucosides and apigenin 7-glucoside.