Matthew Breen

Genetical and physical assignments of equine microsatellites--first integration of anchored markers in horse genome mapping.

Twenty equine microsatellites were isolated from a ge-nomic phage library, and their genetical and physical localization was sought by linkage mapping and fluorescent in situ hybridization (FISH). Nineteen of the markers were found to be polymorphic with, in most cases, heterozygosities exceeding 50%. The markers were mapped in a Swedish reference family for gene mapping, comprising eight half-sib families from Standardbred and Icelandic horse sires. Segregation was analyzed against a set of 35 other markers typed in the pedigree. Thirteen of the microsatellites showed linkage to at least one other marker, with a total of 21 markers being involved in these linkages. In parallel, 18 of the microsatellites could be assigned to their chromosomal region by FISH. These assignments involved eight equine autosomes: ECA1, 2, 4, 6, 9, 10, 15, and 16. The genetical and physical mappings revealed by this study represent a significant extension of the current knowledge of the equine genome map.

Genomic sequences of bovine papillomaviruses in formalin-fixed sarcoids from Australian horses revealed by polymerase chain reaction

Seventy six formalin-fixed paraffin-embedded sarcoids from 62 Australian horses, collected over a ten year period, were examined for the presence of genomic sequences from bovine papillomavirus 1 and 2 (BPV1, BPV2) with the polymerase chain reaction (PCR). Sequences that could be amplified by primers specific for BPV1 and BPV2 were present in 56 of the 76 sarcoids (73%). A restriction site present in BPV1 and absent from BPV2 was detected in 28 of 34 amplified products that were treated with endonuclease.

Identification of papillomaviruses in scrapings from bovine warts by use of the polymerase chain reaction

Bloch, N., Sutton, R.H., Breen, M. and Spradbrow, P.B., 1997. Identification of papillomaviruses in scrapings from bovine warts by use of the polymerase chain reaction. Veterinary Research Communications, 21 (1), 63-68.

BOVINE PAPILLOMAVIRUS TYPE 4 DNA ISOLATED FROM A SKIN LESION IN A STEER

A lesion on the head of a steer, defined histologically as an epithelial papilloma, yielded DNA which did not hybridise with any of the bovine papillomavirus DNAS usually associated with the formation of skin lesions. DNA from the lesion did hybridise with DNA from bovine papillomavirus 4, even under stringent conditions, and contained a sequence that could be amplified by polymerase chain reaction with primers specific for that virus. Bovine papillomavirus 4 had previously been isolated only from lesions of the upper alimentary canal.

Bovine Papillomavirus Type 5: Partial Sequence and Comparison with Other Bovine Papillomaviruses

Two restriction fragments of bovine papillomavirus type 5 (BPV5), of a genomic size of 1.6 and 1.2 kb were subcloned and sequenced. One of them seemed to correspond to the 3′ end of the E1 open reading frame (ORF) and the other to the region of the E7, E8 and 5′ end of the E1 ORF. Alignments of these fragments with other BPVs showed that BPV5 is only distantly related to the other 5 BPVs.