Matus Molcan

Hyperthermic Effect in Suspension of Magnetosomes Prepared by Various Methods

The magnetic properties and hyperthermia effect were studied in solution of magnetosomes obtained by changing conditions during biomineralization of magnetotactic bacteria Magnetospirillum sp.AMB-1. It was shown that adding a higher amount of Wolfes vitamin solution (WVS) or ferric quinate (FQ) cause increase of the mean diameter from 47 nm (normal condition) up to 52 nm and 58 nm respectively. As a consequence of this change the preparation conditions coercivity and Specific Absorption Rate (SAR) increased up to 20 Oe and 949 W/gFe for sample FQ, respectively. On the other hand the process of cultivation at the changed conditions markedly reduced the cultivation time. Also the isolated chains of magnetosome were shorter containing less amount of magnetosomes too.

Magnetic Fluids and Their Complex Systems

The presented chapter provides an overview of selected magnetic nanoparticle systems (magnetic fluids, magnetosomes, magnetoferritin and liquid crystals doped with magnetic particles) as the unique materials with potential utilization in the field of biological and biomedical applications as well as in the field of technology. The main idea for magnetic nanoparticles incorporating is the improvement of material properties and to achieve better conditions for a wide range of scientific disciplines such as magnetic hyperthermia, drug delivery, magneto-optics, power engineering and others.

Effect of magnetosomes on cell proliferation, apoptosis induction and expression of Bcl-2 in the human lung cancer cell line A549

Abstract Magnetosomes are nanoparticles coated with organic membrane produced by magnetotactic bacteria. They appear to be a suitable carrier for the targeted drug transport, which represents a new method of cancer treatment. Advantages of targeted drugs transport are the minimizing of hurt to the organism, specific focusing on certain types of tissues, increased effect of the drug, reduced drug dose and hence side effects. The main goal of the present study was to verify the effect of bacterial magnetic nanoparticles produced by Magnetospirillum strain AMB-1 on cancer cell proliferation, plasmatic membrane integrity, induction of apoptosis as well as on the expression of Bcl-2 in the human lung cancer cell line A549. Obtained results showed that magnetosomes did not have a significant effect on proliferation activity of cells but affected their viability. Moreover, they affected structural integrity of plasmatic membrane and induced apoptosis in cancer cells after their incorporation. The expression of Bcl-2 was also significantly reduced. Taking all these data together, it can be emphasized that magnetosomes severely induce apoptosis in lung cancer cells and after performing the further experiments they could be used in different biomedical applications.