Mauricio O. Zamponi

Importance of Nematocysts in Taxonomy of Acontiarian Sea Anemones (Cnidaria, Actiniaria): A Statistical Comparative Study

Abstract Many papers describe the cnidae from acontiarian sea anemone species, but comparative studies involving significant data amounts, combined with adequate statistical treatment have not been realized. The aim of the present paper is to carry out a comparative study of acontian cnidae from the species Haliplanella lineata (Verrill, 1869), Tricnidactis errans Pires, 1988 (Haliplanellidae) and Anthothoe chilensis (Lesson, 1830) (Sagartiidae). Five living specimens of each species were used; the length of 50 unfired capsules was measured and taken randomly from the following nematocyst categories: b-rhabdoids, p-rhabdoids B1b, p-rhabdoids B2a. A total of 2,000 measurements was done. The data were analyzed with descriptive statistics (mean, standard deviation, median, maximum, minimum, and semiinterquartile range) and Shapiro-Wilks test, Box-Cox transformation, Kruskal-Wallis test and single factor ANOVA. The normal distribution of the dataset must be tested for each nematocyst type in any quantitative study of the cnidae. We conclude that variation in nematocyst size should not be used as an element of conclusive value in the diagnosis of species of acontiarian sea anemones. For these purposes, other characters have to be used to define precisely each taxon, including qualitative variation of the cnidae.

Hydroid populations from sublittoral outcrops off Mar Del Plata, Argentina: Abundance, seasonality and reproductive periodicity

Abstract An analysis is given of the frequency, abundance, seasonality, and reproductive periods of hydroids from sublittoral rocky outcrops in coastal waters off Mar del Plata, Argentina. A total of 13 hydroid species belonging to 8 families were found, 12 of them referable to the subclass Leptothecatae. Species were grouped into three categories based on their occurrence and frequency during the year. Amphisbetia operculata, Sertularella mediterranea and Plumularia setacea were the most abundant hydropolyps in the study area. They occur as distinct clumps in patches having a contagious distribution, although the exact patterns vary considerably. Colonies of A. operculata were observed throughout the year, with gonothecae present between May and January and a reproductive peak at the end of winter. The seasonality of S. mediterranea, with a peak of reproductive activity during the cold period, was similar to that of colonies of the species from the intertidal zone studied previously. Conversely, differences were noted in seasonal patterns of P. setacea between the two zones. Intertidally, this species was found only during the warm season, as small clumps (not more than 35 mm high). In contrast, sub-littoral colonies were larger (up to 130 mm high) and three overlapping generations each year were observed. Comparisons with intertidal hydroid communities are made.

Hydroid assemblages from Mar del Plata, Argentina, at depths between 0 and 500 m. Distribution and biological substrata

The first studies on hydroids of the subantarctic region (SW Atlantic Ocean) were based on samples from expeditions to the Antarctic at the end of the 19th and beginning of the 20th centuries. In spite of these works, hydroids from the northern Argentine continental shelf remained unknown until the 1960s. In coastal waters off Mar del Plata, studies on hydroids have been few in number and limited to species described from preserved material. Even rudimentary ecological information is lacking in these reports. Over the past few decades, samples from oceanographic expeditions, and specimens collected directly from the intertidal rocky shore or by means of scuba diving and snorkeling, have provided heretofore unpublished information on hydropolyps of the Mar del Plata region. The goal of this work is to analyze the hydroid fauna of the Mar del Plata coast, including bathymetric distributions, frequencies of occurrence, and biological substrata. A total of 36 species were included. Most were found at depths shallower than 8 m, where hard substrates predominated. Numbers of species decreased markedly below 80 m. The shallow-water hydroid fauna of Mar del Plata comprises a large number of cosmopolitan or widely distributed species. In deeper zones, species with subantarctic or south hemisphere distributions predominated. Hard bottom outcrops were surrounded by extensive areas of sand. Although such substrata are unfavorable for most hydroids, many species were found in soft bottom areas on polychaete tubes. Stems of hydroids provided the greatest number of epizoic species (18), followed by polychaete tubes (16 species) and sponges (15 species). Hydroids, bivalve mollusks, and sponges were the most frequent substrates. Colonies grew less frequently on bryozoans and tunicates. This general scheme changed at greater depths, where the most frequent substrates were polychaete tubes and sponges.

Hydroid colonies as primary substrata for recruits of the mussel Mytilus edulis platensis front off Mar Del Plata, Argentina

Abstract Due to the sparse presence of algae, hydroid clumps appear as the main primary substrata for the settlement of epizoical mussels in Buenos Aires province (Argentina). The settlement and growth of Mytilus edulis platensis on Amphisbetia operculata and Plumularia setacea colonies during their first life stages was analyzed throughout the year and the double settling larvae phenomenon is discussed. Colonies of both species were frequently colonized by numerous spat, which reached a maximum density of 1370 and 1450 individuals g-1 of colony, respectively. Settlement of Mytilus larvae on the hydroids occurred mainly in November, decreasing in December and January. The plantigrades biomass on A. operculata increased from December until April in spite of a decrease in the number of individuals. In constrast in P. setacea both plantigrade numbers and biomass decreased from December and no individuals were found in February. The major abundance and permanency of plantigrades on A. operculata could be explained by its bigger size and more profuse branching in comparison with P. setacea. The competent Mytilus larvae also settle on mussel beds, but only the filamentous substrata seem to ensure a high probability of survival during the early benthic stages, because it provides protection from predators (sea urchins and sea stars).

Morphological and functional study of the marginal sphincter of the sea anemones Phymactis clematis and Aulactinia marplatensis from intertidal of Mar del Plata, Argentina

It was made the characterization of marginal sphincter to the species Phymactis clematis (Drayton in Dana, 1849) and Aulactinia marplatensis (Zamponi, 1977), from intertidal ecosystem through their morphogical and functional study. The species P. clematis has a circumscript sphincter of palmate type. This muscle is constituted by a mesogloeal axis and several mesogloeal subaxes. Axis as well as subaxes give a support to the endoderm which border is smooth. Aulactinia marplatensis has a circunscript sphincter pinnate type. The axis has a truncated cone shape while in P. clematis the shape is cylindrical on its origin and it is bifurcated at the end. Both species experiments were carried out using the isolated muscles. They were stimulated at increasing KCl concentrations ranging from 20 to 200 mM. The results were analysed in the form of dose-response curves expressed in tension in grams force vs concentration. Contractil force increases in a sigmoid form to increasing KCl concentrations. The correlation between morphology and function and the differences shown in both species would be related to their intertidal distribution.

Biometric and statistical investigations on the cnidoma of the genus Hydra (Cnidaria, Hydrozoa)

This paper deals about the nematocysts like a source of biometric information for comparison between the species Hydra vulgaris Pallas, 1766, Hydra vulgaris pedunculata Deserti et al., 2011 and Hydra pseudoligactis (Hyman, 1931). This biometric tool lets us carry out statistical comparisons and adding these results to the identification of specimens from different classificatory groups. In this particular study, we obtained significant differences between species, individuals of each species and nematocysts type when compared the biometry of its nematocysts. Another result was the variation in of particular nematocysts, like atrichous isorhiza and holotrichous isorhiza for the species H. vulgaris in relation to the column size.