Mauro Wagner de Oliveira

Decomposição e liberação de nutrientes da palhada de cana-de-açúcar em campo

A field decomposition and release of nutrients from trash of sugar cane mechanically harvested and without previous detrashing through burning was evaluated. During the experimental period ¾ August 1996 through August 1997 ¾ rainfall was 1,733 mm and mean maximum and minimum temperatures were 28.0 and 18.0oC, respectively. After one year the mass of trash remained on field decreased of approximately 20%, mostly derived from the decarboxylation of the cell content and hemicelulose. Among all structural carbohydrates only the hemicelulose decomposed. The percentage of K, Ca and Mg released in relation to the total nutrient content in original trash was 85, 44 and 39%, respectively. The N mineralization of the trash was not significant (18%) thus resulting in a negligible statistical difference among the N contents in original and remaining trash

Cardiovascular responses to chemoreflex activation with potassium cyanide or hypoxic hypoxia in awake rats.

Although intravenous (iv) injection of potassium cyanide (KCN) activates the arterial chemoreflex, it has been questioned whether cytotoxic hypoxia reproduces a physiological stimulus such as hypoxic hypoxia (low inspired O2 tension). Thus, the goal of the present study was to compare the cardiovascular responses elicited by intravenous injection of KCN to those caused by hypoxic hypoxia in awake rats before and after bilateral ligature of carotid body arteries. We tested the hypothesis that hypoxic hypoxia activates the cardiovascular chemoreflex just as KCN does, causing an increase in arterial pressure and bradycardia. Intact adult Wistar rats received an intravenous injection of KCN (160 microg/kg) and were exposed to hypoxic hypoxia (7-5% O2 breathing) for 10-15 s at random while mean arterial pressure (MAP) and heart rate (HR) were measured. After the experiments, the animals were submitted to bilateral ligature of carotid body arteries or sham operation and the protocol was repeated on the subsequent day. Before surgery, all rats showed an abrupt rise in arterial pressure accompanied by a marked bradycardia in response to KCN or hypoxic hypoxia, with a very similar pattern. After surgery, these responses persisted only in the sham-operated group and were totally abolished in the ligature group. In conclusion, our data show that KCN is an appropriate stimulus to activate arterial chemoreflex because its cardiovascular responses are comparable to those induced by hypoxic hypoxia. Thus, the use of KCN as a tool to evaluate different aspects of the complex pattern of cardiovascular, respiratory, and behavioural responses to chemoreflex activation seems to be physiologically acceptable.

Degradação da palhada de cana-de-açúcar

Avaliou-se a influencia da aplicacao de ureia e vinhaca na degradacao da lignocelulose e na liberacao dos nutrientes da palhada de cana-de-acucar. Os tratamentos foram: vinhaca (dose equivalente a 100 m3 ha-1) aplicada sobre a palhada, combinada com ureia (dose equivalente a 100 kg ha-1) aplicada sobre a palhada ou enterrada no solo; mistura de cloreto de potassio (dose equivalente a 120 kg ha-1 de K2O) com ureia (dose equivalente a 100 kg ha-1) aplicada sobre a palhada ou enterrada no solo. Durante o periodo experimental, janeiro a novembro de 1997, a palhada recebeu uma lâmina de agua de 1.839 mm, sendo 923 mm de precipitacoes e 916 mm de irrigacoes. As medias das temperaturas maximas e minimas foram 28,4 e 15,5°C, respectivamente. Nao houve efeito dos tratamentos na degradacao da lignocelulose da palhada e na liberacao de nutrientes, verificando-se diferencas estatisticamente significativas apenas entre os resultados da palhada da cana recem colhida e os das remanescentes. Ocorreu reducao de massa de aproximadamente 80% para a hemicelulose e para o conteudo celular, e de 30 e 50% para a lignina e celulose, respectivamente. A porcentagem media de liberacao dos nutrientes N, P, K, Ca, Mg e S, em relacao ao total contido na palhada da cana recem colhida, foi de 18, 67, 93, 57, 68 e 68%, respectivamente.

Myocardial performance in conscious streptozotocin diabetic rats

BackgroundIn spite of a large amount of studies in anesthetized animals, isolated hearts, and in vitro cardiomyocytes, to our knowledge, myocardial function was never studied in conscious diabetic rats. Myocardial performance and the response to stress caused by dobutamine were examined in conscious rats, fifteen days after the onset of diabetes caused by streptozotocin (STZ). The protective effect of insulin was also investigated in STZ-diabetic rats.MethodsCardiac contractility and relaxation were evaluated by means of maximum positive (+dP/dtmax) and negative (-dP/dtmax) values of first derivative of left ventricular pressure over time. In addition, it was examined the myocardial response to stress caused by two dosages (1 and 15 μg/kg) of dobutamine. One-way analysis of variance (ANOVA) was used to compare differences among groups, and two-way ANOVA for repeated measure, followed by Tukey post hoc test, to compare the responses to dobutamine. Differences were considered significant if P < 0.05.ResultsBasal mean arterial pressure, heart rate, +dP/dtmax and -dP/dtmax were found decreased in STZ-diabetic rats, but unaltered in control rats treated with vehicle and STZ-diabetic rats treated with insulin. Therefore, insulin prevented the hemodynamic and myocardial function alterations observed in STZ-diabetic rats. Lower dosage of dobutamine increased heart rate, +dP/dtmax and -dP/dtmax only in STZ-diabetic rats, while the higher dosage promoted greater, but similar, responses in the three groups. In conclusion, the results indicate that myocardial function was remarkably attenuated in conscious STZ-diabetic rats. In addition, the lower dosage of dobutamine uncovered a greater responsiveness of the myocardium of STZ-diabetic rats. Insulin preserved myocardial function and the integrity of the response to dobutamine of STZ-diabetic rats.ConclusionThe present study provides new data from conscious rats showing that the cardiomyopathy of this pathophysiological condition was expressed by low indices of contractility and relaxation. In addition, it was also demonstrated that these pathophysiological features were prevented by the treatment with insulin.

Urea and sugarcane straw nitrogen balance in a soil-sugarcane crop system

Os objetivos deste trabalho foram avaliar a utilizacao do nitrogenio por soqueira de cana-de-acucar de duas fontes, ureia e palha de cana-de-acucar, e a recuperacao do N de ambas as fontes no sistema solo-planta. Os tratamentos foram estabelecidos em blocos casualizados, com quatro repeticoes: T1, mistura de vinhaca e ureia (100 kg ha-1 de N), aplicada em area total sobre o solo coberto com palha de cana-de-acucar marcada com 15N; T2, mistura de vinhaca e ureia (ureia marcada com 15N - ureia-15N; 100 kg ha-1 de N) aplicada em area total sobre o solo coberto com palha de cana-de-acucar nao marcada; e T3, ureia-15N (100 kg ha-1 de N) enterrada em sulcos laterais as linhas de cana-de-acucar, com previa aplicacao de vinhaca sobre o solo sem palha. A vinhaca foi aplicada no volume de 100 m3 ha-1 em todos os tratamentos. O experimento foi realizado em um Podzolico Vermelho-Amarelo (Paleudalf), entre outubro de 1997 e agosto de 1998, em Piracicaba, SP. A eficiencia de uso de N da ureia pela soqueira de cana-de-acucar foi de 21% e a de N da palha de cana-de-acucar foi de 9%. A principal contribuicao do N da palha de cana-de-acucar e a manutencao ou aumento do N orgânico do solo. A tendencia de menor acumulacao, pela cana-de-acucar, do N da ureia no tratamento com palha, foi compensada pela acumulacao de N proveniente da mineralizacao da palha. O N da palha ficou disponivel na segunda metade do ciclo da cultura.

Effect of baroreceptor denervation on the autonomic control of arterial pressure in conscious mice

This study evaluated the role of arterial baroreceptors in arterial pressure (AP) and pulse interval (PI) regulation in conscious C57BL mice. Male animals, implanted with catheters in a femoral artery and a jugular vein, were submitted to sino‐aortic (SAD), aortic (Ao‐X) or carotid sinus denervation (Ca‐X), 5 days prior to the experiments. After basal recording of AP, the lack of reflex bradycardia elicited by administration of phenylephrine was used to confirm the efficacy of SAD, and cardiac autonomic blockade with methylatropine and propranolol was performed. The AP and PI variability were calculated in the time and frequency domains (spectral analysis/fast Fourier transform) with the spectra quantified in low‐ (LF; 0.25–1 Hz) and high‐frequency bands (HF; 1–5 Hz). Basal AP and AP variability were higher after SAD, Ao‐X or Ca‐X than in intact mice. Pulse interval was similar among the groups, whereas PI variability was lower after SAD. Atropine elicited a slight tachycardia in control mice but did not change PI after total or partial denervation. The bradycardia caused by propranolol was higher after SAD, Ao‐X or Ca‐X compared with intact mice. The increase in the variability of AP was accompanied by a marked increase in the LF and HF power of the AP spectra after baroreceptor denervation. The LF and HF power of the PI were reduced by SAD and by Ao‐X or Ca‐X. Therefore, both sino‐aortic and partial baroreceptor denervation in mice elicits hypertension and a remarkable increase in AP variability and cardiac sympathetic tonus. Spectral analysis showed an important contribution of the baroreflex in the power of LF oscillations of the PI spectra. Both sets of baroreceptors seem to be equally important in the autonomic regulation of the cardiovascular system in mice.

Pyridostigmine Restores Cardiac Autonomic Balance after Small Myocardial Infarction in Mice

The effect of pyridostigmine (PYR) - an acetylcholinesterase inhibitor - on hemodynamics and cardiac autonomic control, was never studied in conscious myocardial infarcted mice. Telemetry transmitters were implanted into the carotid artery under isoflurane anesthesia. Seven to ten days after recovery from the surgery, basal arterial pressure and heart rate were recorded, while parasympathetic and sympathetic tone (ΔHR) was evaluated by means of methyl atropine and propranolol. After the basal hemodynamic recording the mice were subjected to left coronary artery ligation for producing myocardial infarction (MI), or sham operation, and implantation of minipumps filled with PYR or saline. Separate groups of anesthetized (isoflurane) mice previously (4 weeks) subjected to MI, or sham coronary artery ligation, were submitted to cardiac function examination. The mice exhibited an infarct length of approximately 12%, no change in arterial pressure and increased heart rate only in the 1st week after MI. Vagal tone decreased in the 1st week, while the sympathetic tone was increased in the 1st and 4th week after MI. PYR prevented the increase in heart rate but did not affect the arterial pressure. Moreover, PYR prevented the increase in sympathetic tone throughout the 4 weeks. Concerning the parasympathetic tone, PYR not only impaired its attenuation in the 1st week, but enhanced it in the 4th week. MI decreased ejection fraction and increased diastolic and systolic volume. Therefore, the pharmacological increase of peripheral acetylcholine availability by means of PYR prevented tachycardia, increased parasympathetic and decreased sympathetic tone after MI in mice.

Changes in autonomic control of the cardiovascular system in the Wistar audiogenic rat (WAR) strain

We evaluated autonomic cardiovascular modulation and baroreflex control of heart rate (HR) in a particular epileptic rat strain, Wistar audiogenic rats (WARs). We studied spontaneous baroreflex sensitivity as well as reflex changes in HR evoked by phenylephrine/nitroprusside-induced changes in arterial pressure (AP). Atropine and propranolol were used to measure cardiac autonomic tone. AP and pulse interval (PI) variability analysis were performed in the time and frequency domains (FFT spectral analysis) to evaluate cardiovascular sympatovagal modulation in WARs. AP and HR were higher in WARs (109±2 mm Hg and 366±9 bpm) than in Wistar control rats (101±2 mm Hg and 326±10 bpm). The power of the low-frequency band of both AP and PI spectra, a marker of sympathetic modulation, was higher in WARs than in Wistar control rats. The high-frequency power of the PI spectra in normalized units, which is linked to cardiac vagal modulation, was lower in WARs. Both WARs and Wistar control rats had similar vagal tone (91±13 bpm vs 94±11 bpm, respectively), but sympathetic tone was higher in WARs (30±4 bpm vs 14±4 bpm). No differences were detected in the gain of evoked (1.32±0.1 ms/mm Hg vs 1.35±0.2 ms/mm Hg) or spontaneous (1.34±0.2 ms/mm Hg vs 2.04±0.2 ms/mm Hg) baroreflex sensitivity. The higher AP and HR and the autonomic imbalance (sympathetic predominance) in WARs might be associated with an increased risk of life-threatening cardiovascular events in this strain.

Hemodynamic and ventilatory response to different levels of hypoxia and hypercapnia in carotid body-denervated rats

OBJECTIVE: Chemoreceptors play an important role in the autonomic modulation of circulatory and ventilatory responses to changes in arterial O2 and/or CO2. However, studies evaluating hemodynamic responses to hypoxia and hypercapnia in rats have shown inconsistent results. Our aim was to evaluate hemodynamic and respiratory responses to different levels of hypoxia and hypercapnia in conscious intact or carotid body-denervated rats. METHODS: Male Wistar rats were submitted to bilateral ligature of carotid body arteries (or sham-operation) and received catheters into the left femoral artery and vein. After two days, each animal was placed into a plethysmographic chamber and, after baseline measurements of respiratory parameters and arterial pressure, each animal was subjected to three levels of hypoxia (15, 10 and 6% O2) and hypercapnia (10% CO2). RESULTS: The results indicated that 15% O2 decreased the mean arterial pressure and increased the heart rate (HR) in both intact (n = 8) and carotid body-denervated (n = 7) rats. In contrast, 10% O2 did not change the mean arterial pressure but still increased the HR in intact rats, and it decreased the mean arterial pressure and increased the heart rate in carotid body-denervated rats. Furthermore, 6% O2 increased the mean arterial pressure and decreased the HR in intact rats, but it decreased the mean arterial pressure and did not change the HR in carotid body-denervated rats. The 3 levels of hypoxia increased pulmonary ventilation in both groups, with attenuated responses in carotid body-denervated rats. Hypercapnia with 10% CO2 increased the mean arterial pressure and decreased HR similarly in both groups. Hypercapnia also increased pulmonary ventilation in both groups to the same extent. CONCLUSION: This study demonstrates that the hemodynamic and ventilatory responses varied according to the level of hypoxia. Nevertheless, the hemodynamic and ventilatory responses to hypercapnia did not depend on the activation of the peripheral carotid chemoreceptors.

Autonomic cardiocirculatory control in mice with reduced expression of the vesicular acetylcholine transporter

In cardiovascular diseases, sympathetic tone has been comprehensively studied, whereas parasympathetic tone has received minor attention. The vesicular ACh transporter (VAChT) knockdown homozygous (VAChT KD(HOM)) mouse is a useful model for examining the cardiocirculatory sympathovagal balance. Therefore, we investigated whether cholinergic dysfunction caused by reduced VAChT expression could adversely impact hemodynamic parameter [arterial pressure (AP) and heart rate (HR)] daily oscillation, baroreflex sensitivity, hemodynamic variability, sympathovagal balance, and cardiovascular reactivity to restraint stress. Wild-type and VAChT KD(HOM) mice were anesthetized for telemetry transmitter implantation, and APs and HRs were recorded 10 days after surgical recovery. Changes in HR elicited by methylatropine and propranolol provided the indexes of sympathovagal tone. Cardiovascular reactivity in response to a restraint test was examined 24 h after continuous recordings of AP and HR. VAChT KD(HOM) mice exhibited reduced parasympathetic and elevated sympathetic tone. Daily oscillations of AP and HR as well as AP variability were similar between groups. Nevertheless, HR variability, patterns with two dissimilar variations from symbolic analysis, and baroreflex sensitivity were reduced in VAChT KD(HOM) mice. The change in mean AP due to restraint stress was greater in VAChT KD(HOM) mice, whereas the tachycardic response was not. These findings demonstrate that the cholinergic dysfunction present in the VAChT KD(HOM) mouse did not adversely impact basal hemodynamic parameters but promoted autonomic imbalance, an attenuation of baroreflex sensitivity, and a greater pressure response to restraint stress. These results provide a framework for understanding how autonomic imbalance impacts cardiovascular function.