Maxime Girot

Characterization of cellular mechanical behavior at the microscale level by a hybrid force sensing device

This paper deals with the development of an open design platform for characterization of mechanical cellular behavior. The resulting setup combines Scanning Probe Microscopy (SPM) techniques and advanced robotic approaches in order to carry out both prolonged observations and spatial measurements on biological samples. Visual and force feedback is controlled to achieve automatic data acquisition and to monitor process when high skills are required. The issue of the spring constant calibration is addressed using an accurate dynamic vibration approach. Experimentation on the mechanical cell characterization under in vitro conditions on human adherent Epithelial Hela cells demonstrates the viability and effectiveness of the proposed setup. Finally, the JKR (Johnson, Kendall and Roberts), the DMT (Derjaguin, Muller and Toporov) and Hertz contact theories are used to estimate the contact area between the cantilever and the biological sample.

Modeling Soft Contact Mechanism of Biological Cells Using an Atomic Force Bio-Microscope

The development of a mechanical force sensing device system based on force/vision feedback control for exploring in vitro the contact mechanics of human adherent cervix Epithelial Hela cells is presented in this paper. The design of the prototype combines scanning probe microscopy (SPM) techniques with advanced robotics approaches. Some important issues in the design process, such as in vitro environment constraints and calibration of the force sensing probe are also addressed in this paper. The system is then used for accurate and non-destructive mechanical characterization based on soft contact interactions on biological samples. Finally, some mechanical properties of the studied biological samples are estimated using two appropriate models describing the contact mechanism taking into account adhesion forces

An Hybrid Micro-Force Sensing Device for Mechanical Cell Characterization

This paper presents a fully automated microrobotic system based on force/vision referenced control designed for cell mechanical characterization. The design of the prototype combines scanning probe microscopy (SPM) techniques with advanced robotics approaches. As a result, accurate and non-destructive mechanical characterization based on soft contact interactions mechanics are achieved. The in vitro working conditions are supported by the experimental setup so that mechanical characterizations can be performed in biological environmental requirements as well as in cyclical operating mode during several hours. The design and calibration of the different modules which compose the experimental setup are detailed. Experimentation on the mechanical cell characterization under in vitro conditions on human adherent cervix Epithelial Hela cells are presented to demonstrate the viability and effectiveness of the proposed setup

Towards a non-destructive in vitro biomechanical characterization

This paper presents a fully automated micro-robotic system based on force/vision referenced control designed for cell mechanical characterization. The design of the prototype combines Scanning Probe Microscopy (SPM) techniques with advanced robotics approaches. As a result, accurate and non-destructive mechanical characterization based on soft contact interactions mechanics are achieved. The in vitro working conditions are supported by the experimental setup so that mechanical characterizations can be performed in biological environmental requirements as well as in cyclical operating mode during several hours. The design and calibration of the different modules which compose the experimental setup are detailed. Experimentation on the mechanical cell characterization under in vitro conditions on human adherent cervix Epithelial Hela cells are presented to demonstrate the viability and effectiveness of the proposed setup

A Microforce and Nanoforce Biomicroscope Device for In Vitro Mechanotransduction Investigation

This paper deals with the development of an open design platform for explorative cell mechanotransduction investigation. The produced setup combines scanning probe microscopy (SPM) techniques and advanced robotics approaches, allowing both prolonged observations and spatial measurements on biological samples. As a result, an enhanced force probing method based on scanning microscopy techniques and advanced robotics and automation approaches is integrated in this device. Visual and force feedback control is used to achieve automatic data acquisition and monitoring processes when high skills are required. Experimentation on the mechanical cell characterization under in vitro conditions on human adherent cervix epithelial Hela cells are presented to demonstrate the viability and effectiveness of the proposed setup.

Haptic Rendering of Biological Elastic Properties based on Biomechanical Characterization

This paper deals with the design of a micro-force sensing device for biomechanical characterization of biological samples. This device combines (SPM) techniques and advanced robotics approaches and allows to carry out in vitro prolonged observations as well as biomechanical characterization experiments. Elastic properties of biological samples are reflected to the macroscale during the mechanical characterization process by means of a haptic sensing device. Non-linear elasticity theory formalism is used in order to achieve realistic elastic rendering. Mechanical characterization experiments are conducted on human tumoral Epithilial Hella cells in order to demonstrate the efficiency and viability of the proposed system

Enhanced Near-Field Force Probing for In Vitro Biomechanical Characterization

This paper presents a fully automated micro-robotic system based on force/vision referenced control designed for mechanical cell characterization. The design of the prototype combines scanning probe microscopy (SPM) techniques with advanced robotics approaches. As a result, accurate and non-destructive mechanical characterization based on soft contact mechanisms are achieved. The in vitro working conditions are supported by the experimental setup so that mechanical characterization can be performed in biological environmental requirements as well as in cyclical operating mode during several hours. The design of the different modules which compose the experimental setup is detailed. Mechanical cell characterization experiments under in vitro conditions on human cervix epithelial Hela adherent cells are presented to demonstrate the viability and effectiveness of the proposed setup

A robotic platform for targeted studies on biological cells

This paper deals with the development of an open design platform for explorative cell mechanotransduction investigation. The produced setup combines SPM (Scanning Probe Microscopy) techniques and advanced robotics approaches allowing both prolonged observations and spatial measurements on biological samples. As a result, an enhanced force probing method based on scanning microscopy techniques and advanced robotics and automation approaches are integrated in this device. Visual and force feedback control are used to achieve automatic data acquisition and monitoring process when high skills are required. Experimentation on the mechanical cell characterization under in vitro conditions on human adherent cervix Epithelial Hela cells are presented to demonstrate the viability and effectiveness of the proposed setup.

Elastic Properties Exploration of In Vitro Cultured Microscopic Cells based on Haptic Sensing

This paper deals with the design of a micro-force sensing device for biomechanical characterization of biological samples. This device combines (SPM) techniques and advanced robotics approaches and allows to carry out in vitro prolonged observations as well as biomechanical characterization experiments. Elastic properties of biological samples are reflected to the macroscale during the mechanical characterization process by means of a haptic sensing device. Non-linear elasticity theory formalism is used in order to achieve realistic elastic rendering. Mechanical characterization experiments are conducted on human tumoral Epithilial Hella cells in order to demonstrate the efficiency and viability of the proposed system

The force sensing bio-microscope: an efficient tool for cells mechanotransduction studies.

This paper deals with the development of an open design platform for explorative cells mechanotransduction investigation. The produced setup combines SPM techniques and advanced robotics approaches allowing to carry out both prolonged observations and spatial measurements on biological samples. As a result, enhanced force probing method based on scanning microscopy techniques and advanced robotics/automation approaches are integrated in this device. Visual and force feedback control are used to achieve automatic data acquisition and monitoring process when high skills are required. Preliminary in vitro experiments on human promyelocytic leukemia cells (NB4) are conducted in order to demonstrate the viability of the proposed design. Some relevant mechanical cell properties are extracted such as elasticity and viscosity parameters