Mayda González

UGT2B17 minor histocompatibility mismatch and clinical outcome after HLA-identical sibling donor stem cell transplantation.

Minor histocompatibility Ags (mHags) have been implicated in the pathogenesis of GVHD after allogeneic hematopoietic stem cell transplantation (HSCT). Uridine diphospho-glucuronosyltransferase 2B17 (UGT2B17) gene deletion may act as a mHag and its association with acute GVHD (aGVHD) has been described. We retrospectively studied the clinical impact of a UGT2B17 mismatch in a cohort of 1127 patients receiving a HSCT from an HLA-identical sibling donor. UGT2B17 mismatch was present in 69 cases (6.1%). Incidence of severe aGVHD was higher in the UGT2B17 mismatched pairs (22.7% vs 14.6%), but this difference was not statistically significant (P: 0.098). We did not detect differences in chronic GVHD, overall survival, relapse-free survival, transplant-related mortality or relapse. Nevertheless, when we analyzed only those patients receiving grafts from a male donor (616 cases), aGVHD was significantly higher in the UGT2B17 mismatched group (25.1% vs 12.8%; P: 0.005) and this association was confirmed by the multivariate analysis (P: 0.043; hazard ratio: 2.16, 95% confidence interval: 1.03–4.57). Overall survival was worse for patients mismatched for UGT2B17 (P: 0.005). We conclude that UGT2B17 mismatch has a negative clinical impact in allogeneic HSCT from HLA-identical sibling donors only when a male donor is used. These results should be confirmed by other studies.

TWO NEW SPECIES OF CLAVELLA (COPEPODA, SIPHONOSTOMATOIDA, LERNAEOPODIDAE) AND A NEW SPECIES OF LOPHOURA (COPEPODA, SIPHONOSTOMATOIDA, SPHYRIIDAE): PARASITES ON THE DEEP-WATER FISH, NEZUMIA PULCHELLA FROM THE NORTHERN CHILEAN COAST

[Three new species of parasitic copepods were isolated from the macrourid fish, Nezumia pulchella (Pequeno, 1971), caught along the northern Chilean coast, and are described and illustrated herein. Clavella singularis n. sp. is characterized by the shape and armature of the second antenna, and, by a combination of characteristics, can be distinguished from all other congeneric species bearing a genital process. Clavella fortis n. sp. is characterized by a strong cephalosome and by a combination of characteristics that also differentiates it from its congeners with a genital process. The new species, Lophoura unilobulata is characterized by the presence of a simple holdfast, with one lobe on each side of the neck. With this report, the number of reported Clavella species parasitizing fish from the Chilean coast increases to nine. In addition, the L. unilobulata reported herein represents the third record of a Lophoura species parasitizing a species of the genus Nezumia and represents the first record of the genus in the southeastern Pacific ocean; it is also the third record of a species of the family Sphyriidae occurring along the Chilean coast. Tres nuevas especies de copepodos parasitos sobre un macrourido, Nezumia pulchella desde la costa norte de Chile son descritas e ilustradas. Clavella singularis n. sp. se caracteriza por la forma y armadura de la segunda antena y puede ser distinguida de todas sus congeneres, con proceso genital, por una combinacion de caracteres. Clavella fortis n. sp. se caracteriza por su fuerte cefalosoma, es tambien diferenciada de sus congeneres con proceso genital, por una combinacion de caracteristicas. La nueva especie Lophoura unilobulata se caracteriza por la presencia de una estructura de anclaje simple, con solo un lobulo sobre cada lado de su cuello. Con este reporte el numero de especies de Clavella en la costa de Chile se incrementa a nueve. L. unilobulata es el tercer registro de una especie de Lophoura parasitando a especies de Nezumia. Adicionalmente, este descubrimiento representa el primer registro del genero en el Pacifico Sur Este, y es la tercera especie de Sphyriidae habitando la costa de Chile., Three new species of parasitic copepods were isolated from the macrourid fish, Nezumia pulchella (Pequeno, 1971), caught along the northern Chilean coast, and are described and illustrated herein. Clavella singularis n. sp. is characterized by the shape and armature of the second antenna, and, by a combination of characteristics, can be distinguished from all other congeneric species bearing a genital process. Clavella fortis n. sp. is characterized by a strong cephalosome and by a combination of characteristics that also differentiates it from its congeners with a genital process. The new species, Lophoura unilobulata is characterized by the presence of a simple holdfast, with one lobe on each side of the neck. With this report, the number of reported Clavella species parasitizing fish from the Chilean coast increases to nine. In addition, the L. unilobulata reported herein represents the third record of a Lophoura species parasitizing a species of the genus Nezumia and represents the first record of the genus in the southeastern Pacific ocean; it is also the third record of a species of the family Sphyriidae occurring along the Chilean coast. Tres nuevas especies de copepodos parasitos sobre un macrourido, Nezumia pulchella desde la costa norte de Chile son descritas e ilustradas. Clavella singularis n. sp. se caracteriza por la forma y armadura de la segunda antena y puede ser distinguida de todas sus congeneres, con proceso genital, por una combinacion de caracteres. Clavella fortis n. sp. se caracteriza por su fuerte cefalosoma, es tambien diferenciada de sus congeneres con proceso genital, por una combinacion de caracteristicas. La nueva especie Lophoura unilobulata se caracteriza por la presencia de una estructura de anclaje simple, con solo un lobulo sobre cada lado de su cuello. Con este reporte el numero de especies de Clavella en la costa de Chile se incrementa a nueve. L. unilobulata es el tercer registro de una especie de Lophoura parasitando a especies de Nezumia. Adicionalmente, este descubrimiento representa el primer registro del genero en el Pacifico Sur Este, y es la tercera especie de Sphyriidae habitando la costa de Chile.]

Effect of mismatching for mHA UTA2-1 on clinical outcome after HLA-identical sibling donor allo-SCT.

Effect of mismatching for mHA UTA2-1 on clinical outcome after HLA-identical sibling donor allo-SCT

486 Therapeutic Effect and Security in Asthmatics Adult Patients Treated with Dermatophagoides Pteronyssinus Allergen Sublingual Immunotherapy.

Background The specific active immunotherapy, employing vaccine of allergen of mite is a treatment considered as effective for the respiratory allergy and asthma. The sublingual route has minor risk of systematises reactions. The objective of this study was to determine the therapeutic effect and security of sublingual immunotherapy (ITSL) employing the standard vaccine VALERGEN-DP (BIOCEN, CUBA) in a population of asthmatic Cuban patients. Methods A phase II Clinical Trials double blind, placebo controlled in a total of 40 adult patients with mild or moderate asthma and specific sensibility preponderant to this mite. Half of patients received drops by sublingual route with growing doses up to 2000 UB. Results The treatment was effective in the reduction of clinical symptoms and medication intake as compared to conventional treatment in control group. The cutaneous sensibility to this mite was significant reduced, increasing in 1.9 log; the amount of necessary allergen to provoke a positive Prick Test. An improvement of the lung function was observed with a significant reduction (P < 0.05) of expiratory pick flow variability. The frequency of local reactions were only 0.58% of administration. Conclusions The VALERGEN-DP vaccine is an effective treatment and profitable against asthma in our population and guarantee its generalization in the Allergy Services of our health system.

485 Therapeutic Effect and Safety of Tropical Mite Allergen Vaccines by Subcutaneous Route in Allergic Asthmatics Patients

Background Allergen-specific subcutaneous immunotherapy (SCIT) is presently recognized as a biological response modifier, as it is the only available treatment able to influence the natural course of allergic disease. Extensive clinical evidence supports its efficacy. Safety concerns are related to the risks of anaphylactic reactions during treatment. Standardization of allergen vaccines in terms of allergenic activity allows a more precise control over the administered doses and can be, therefore, very relevant for both efficacy and safety of SCIT. House Dust Mites (HDM), particularly Dermatophagoides pteronyssinus (Dp), Dermatophagoides siboney (Ds) and Blomia tropicalis (Bt) have been described as very relevant allergen sources in Cuba, with a strong association to respiratory allergy symptoms. Objective To asses the efficacy and safety of standardized allergen vaccines of these 3 mite species (Valergen, Biocen, Cuba) in Cuban asthmatic patients. Methods Three Double-Blind Placebo-Controlled clinical trials were performed in 40 patients each, showing asthmatic symptoms and positive predominant Skin Prick Test (SPT) to each mite, respectively. Half of patients received the active treatment consisting of subcutaneous injections with increasing doses, up to 6000 BU. Results The total 1 year cumulative dose was 63035 BU, in an average of 20.5 injections. The treatment was effective in the reduction of clinical symptoms (up to 32%, 95%CI: 28-36%; P = 0.0006) and medication intake (23%, 95%CI:18-28%), as compared to control treatment. The skin sensitivity to the allergens decreased significantly (P = 0.0001), with regard to the beginning of the treatment. The allergen amount needed to induce a positive SPT increased 297-fold. An improvement of the lung function was observed, expressed in a modest Peak-Expiratory-Flow increase (P < 0.05) and reduction of PEF daily variability. SIT was considered effective in 71% of patients. The frequency of local adverse reactions was 2.4 % of injections. Conclusions The results indicates that immunotherapy, using standardized House Dust Mite vaccines, including tropical species, is effective and safe for the control and amelioration of the asthma in our population.