Md. Iqbal Hosen

Molecular phylogenetic analyses redefine seven major clades and reveal 22 new generic clades in the fungal family Boletaceae

Mushrooms in the basidiomycete family Boletaceae are ecologically and economically very important. However, due to the morphological complexity and the limited phylogenetic information on the various species and genera of this fungal family, our understanding of its systematics and evolution remains rudimentary. In this study, DNA sequences of four genes (nrLSU, tef1-α, rpb1, and rpb2) were newly obtained from ca. 200 representative specimens of Boletaceae. Our phylogenetic analyses revealed seven major clades at the subfamily level, namely Austroboletoideae, Boletoideae, Chalciporoideae, Leccinoideae, Xerocomoideae, Zangioideae, and the Pulveroboletus Group. In addition, 59 genus-level clades were identified, of which 22 were uncovered for the first time. These 22 clades were mainly placed in Boletoideae and the Pulveroboletus Group. The results further indicated that the characters frequently used in the morphology-based taxonomy of Boletaceae, such as basidiospore ornamentation, the form of the basidioma, and the stuffed pores each had multiple origins within the family, suggesting that the use of such features for high-level classification of Boletaceae should be de-emphasized and combined with other characters.

Borofutus, a new genus of Boletaceae from tropical Asia: phylogeny, morphology and taxonomy

A new monotypic genus in the Boletaceae, Borofutus, typified by B. dhakanus, is described using morphological and molecular evidence. This is a putatively ectomycorrhizal fungus associated with Shorea robusta. Borofutus is characterized by the combination of the following characters: basidiomata small to medium-sized; pileus grayish brown to cocoa brown; hymenophore subdecurrent, cream then golden brown, with broad, nearly hexagonal pores; basidiospores purple to purplish red in H2O, ornamented with irregular to regular shallow pits; cystidia lageniform, thick-walled. Borofutus is sister to Spongiforma in molecular phylogenetic analyses using DNA nucleotide sequences of single or multiple loci. A description, line drawings, phylogenetic placement and comparison with allied taxa are presented herein.

Amanita cinereovelata , a new species of Amanita section Lepidella from Bangladesh

Amanita cinereovelata is described and illustrated as a new species of Amanita sect. Lepidella from Bangladesh. The species is characterized by its small to medium-sized basidiomata, gray to lilac-gray pulverulent-floccose volval remnants on the pileus, a bulbous stipe base, globose to subglobose amyloid basidiospores, the presence of clamps at the bases of basidia, and an association with Shorea robusta. Molecular phylogenetic analysis indicates that A. cinereovelata is a species sister to A. eriophora, A. longipes, A. tephrea and A. vestita. Comparisons with morphologically similar taxa are provided.

Clarkeinda trachodes (Agaricales, Basidiomycetes), first record from Bangladesh

An interesting agaric, Clarkeinda trachodes, is reported for the first time from Bangladesh. A full description, discussion, and illustrations are provided. The enigmatic agaric genus, currently known only from south and southeast Asia, is characterized by the presence of a fawn colored pellicle on the central pileus surface, a stipe with a superior annulus and basal volva, and thick-walled pigmented spores with a slightly depressed truncated apex. Our examination of a voucher specimen from Italy indicates that a recent report of Clarkeinda in Europe was based on a misidentified Agaricus collection.

Coniolepiota spongodes (Agaricaceae, Basidiomycota) in Bangladesh and China

The genus Coniolepiota is recorded for the first time from Bangladesh and China. Coniolepiota spongodes is characterized by its gray lilac to purple floccose basidiomata and relatively small smooth ellipsoid to oblong basidiospores. A detailed morphological description, photographs and line drawings, with confirmation of the phylogenetic position of newly reported taxon are provided herein.

Amanita tullossiana, a new species, and two new records of Amanita section Lepidella from north-western Himalaya, India

Abstract Amanitatullossiana, a new species of Amanita [subgenus Lepidella] section Lepidella from India is described. The species is characterised by its ash grey to brownish-grey pileus covered with dark grey to greyish-black universal veil remnants, the upper part of its rooting stipe base covered by several rows of recurved scales, broadly ellipsoid to ellipsoid basidiospores, absence of basidial clamp connections and pileal remnants of universal veil comprising abundant, disordered inflated cells intermixed with scattered filamentous hyphae. Molecular phylogenetic analysis and morphology both support the association of A.tullossiana with species of Bas’ stirps Cinereoconia – A.cinereoconia and A.griseoverrucosa. Two species, A.griseoverrucosa and A.virgineoides are reported here as new records for India.

Two new species of Phylloporus from Bangladesh, with morphological and molecular evidence

ABSTRACT Two new species of Phylloporus from Bangladesh forests dominated by Shorea robusta are described and illustrated, based on morphological and molecular phylogenetic evidence. Phylloporus attenuatus is characterized by its wine-red to brownish-red pilei, decurrent lamellae that stain blue, downwards tapering stipe, a trichodermium pileipellis, and ellipsoidal-ovoid to ellipsoidal basidiospores (7–8 × 4–5 µm). Phylloporus catenulatus is characterized by its small-sized basidiomata with reddish-brown pilei, narrowly decurrent with distant lamellae that stain blue, a subepithelium pileipellis, and ellipsoidal to ellipsoidal-ovoid basidiospores (8–9.5 × 4–5 µm). Both new species of Phylloporus are distinctive from any known taxa based on phylogenetic analyses of partial sequences of the nuc rDNA internal transcribed spacer (ITS; including ITS1-ITS2 but not 5.8S) region, partial sequence of nuclear ribosomal large subunit (28S) domains D1 and D2, and translation elongation factor 1-α (TEF1α). Both species are compared with phenotypically similar taxa and illustrated with line drawings and photographs. A key to the species of Phylloporus known from Bangladesh is provided.