Megumi Miyazawa

Food‐dependent exercise‐induced anaphylaxis: influence of concurrent aspirin administration on skin testing and provocation

Summary Background Provocation tests in patients with food‐dependent exercise‐induced anaphylaxis (FDEIA) are often negative, even after a sufficient quantity of the implicated food and exercise have been taken.

Cutaneous Monomorphous CD4- and CD56-Positive Large-Cell Lymphoma

Background: Recently, CD56 (NCAM)-positive lymphomas, such as nasal and nasal-type angiocentric NK/T cell lymphoma, aggressive NK cell leukemia/lymphoma and blastic NK cell lymphoma, were described by several authors as a unique group of lymphoma. Objective: In this study, we intend to clarify the clinicopathological features of cutaneous CD4+ and CD56+ lymphoma. Methods: Four patients with cutaneous CD4+ and CD56+ lymphoma were studied. Results: Age at the first examination ranged from 71 to 89 years (mean = 81.2 years). One patient was female and 3 were males. The organ mainly involved at presentation was the skin. Lymphadenopathy, splenomegaly, leukemic spread and central nervous system involvement were observed as the disease progressed. The mean survival time was 12.2 months. Epstein-Barr virus was not detected within the tumor cells. Conclusion: This peculiar lymphoma is different from nasal and nasal-type angiocentric NK/T cell lymphoma and aggressive NK cell leukemia/lymphoma. Similar cases have been reported as blastic NK cell lymphoma/leukemia.

Adult T-cell leukemia/lymphoma (ATL) –clinical, histopathological, immunological and immunohistochemical characteristics

Abstract Twenty‐one patients with ATL were assessed. The predominant physical findings were lymph node and bone marrow involvement, skin involvement, hepatosplenomegaly and leukemic manifestations. The predominant histopathological findings in both skin and lymph node specimens were the diffuse medium‐sized cell type and the diffuse mixed cell type. Some phenotypic discrepancy was found between the neoplastic cells in the peripheral blood, lymph nodes and skin of patients with ATL with respect to CD45RA and CD45RO, and CD7, CD29, CD25 and HLA‐DR. That is, the predominant neoplastic cell phenotype was the helper T‐cell, which was CD3+, CD4+, CD7+, CD25+, CD45RA+ and HLA‐DR+, and CD29− and CD45RO− in peripheral blood and lymph nodes, and CD3+, CD4+, CD7+, CD29+, CD45RO+ and HLA‐DR+, and CD45RA− in the skin. In other words, we have described the phenotypic heterogeneity of ATL cells and demonstrated the heterogeneity of CD45R isoform expression on ATL cells in different organs – the skin, peripheral blood and lymph nodes – of the same patient.

Cutaneous B-cell lymphoma--a clinical, pathological and immunohistochemical study.

Eleven cases of cutaneous B‐cell lymphoma (CBCL) were studied. The ages at presentation ranged from 34 to 79 years (mean = 59·9 years). Six patients were female and five male. Five of the 11 patients had a solitary tumour and the other six had multiple tumours at initial presentation. According to Burgs classification, six cases were at stage I, two stage II, two stage III and one was at stage IV at initial presentation. Abnormalities in laboratory data were rare, except for serum lactic dehydrogenase values. Epidermotropism was not detected, and the area mainly affected by neoplastic cells was the reticular dermis (seven cases) and suhcutis (four cases). Biopsy‐specimens from the patients analysed by immunohisiochemical techniques on paraffin or cryostat sections showed CD20 and/or CD22 positivity. Biopsy specimens from two patients which showed CD 10 positivity were diffuse large cell types by the working formulation and presented as pre‐B‐cell lymphoma. At least two groups of CBCL were demonstrable on the basis of prognosis. One was a benign low‐grade lymphoma presenting with solitary tumours, mature B‐cell markers and intermediate‐grade pathology, and the other was a high‐grade lymphoma with multiple tumours, pre‐B‐cell or mature B‐cell markers and a poor prognosis.

A case of cutaneous B-cell lymphoma treated successfully with MACOP-B.

A case of cutaneous B‐cell lymphoma successfully treated by MACOP‐B therapy is described. The patient was a 43‐year‐old man with reddish tumors measuring 3 to 7 cm in diameter on the right cheek and the post‐auricles. Histopathologically, massive infiltrations of medium‐sized atypical lymphoid cells were found in the reticular dermis and subcutis. A clear zone beneath the epidermis was also detected. The atypical lymphoid cells were positive for CD19, CD20, CD22 and HLA‐DR but negative for CD3, CD4, CD5, CD10, CD43, CD45RO and CDw75. The patient was treated successfully with the MACOP‐B protocol from March of 1990 to May of 1990. Since April of 1990, he has been free of disease.

A Case of Cutaneous B‐cell Lymphoma with a Storiform Stromal Reaction

A case of cutaneous B‐cell lymphoma is described. The patient was treated only by surgical excision of the skin tumors five times during a period of about two years from February of 1984 to October of 1986. After the last surgical excision, a continuous disease‐free period was achieved. Biopsy samples showed dense lymphocytic infiltrations with discrete masses in the dermis and subcutis; one of them showed a storiform pattern. At the time, the infiltrating cells were composed of medium and large lymphoid cells and spindle‐shaped cells. The medium and large lymphoid cells were positive for CD20, CD22 and HLA‐DR and negative for CD3, CD4, CD5, CD8, CD43, and kappa and lambda light chain. The spindle‐shaped cells were negative for CD20, CD43, kappa and lambda light chain, lysozyme, and S‐100 protein.

Low Expression of Adhesion Molecules in a Case of Cutaneous T-cell Lymphoma

A case of cutaneous T‐cell lymphoma (CTCL) with low expression of the adhesion molecules lymphocyte function‐associated antigen‐1 (LFA‐1), intercellular adhesion molecule‐1 (ICAM‐1), and very late antigen‐4 (VLA‐4) is described. The patient was a 90‐year‐old man with red round homogeneous tumors on his scalp, trunk, and extremities. He had no history of definite erythema or plaque stage. A biopsy sample taken from a tumor revealed massive infiltration of atypical lymphocytes in the reticular dermis and subcutis with a definite clear zone. The atypical lymphocytes were medium‐sized with slightly convoluted nuclei. Immunohistochemically, the infiltrates showed the phenotype of so‐called memory T cells. On the basis of these features, the case was diagnosed as CTCL. Expression of LFA‐1, ICAM‐1 and VLA‐4 on the infiltrates was 9%, 13% and 11%, respectively, which is much lower than that in classic mycosis fungoides. This finding suggests that loss of these adhesion molecules may contribute to loss of epidermotropism in the advanced stage of CTCL.

Successful Treatment of Adult T‐cell Leukemia/Lymphoma with MACOP‐B, M‐FEPA and VEPP‐B Combination Chemotherapy

A 45‐year‐old man was referred to our department in March of 1989. Physical examination showed erythroderma, palmo‐plantar hyperkeratosis, generalized lymphadenopathy, hepatosplenomegaly, and leukemic manifestation. The lymphocyte count in the peripheral blood before treatment was 1.7 × 104 cells/mm3. Atypical lymphocytes such as flower cells and lobulated cells were seen in the peripheral blood. A sample excised from a lymph node showed immunoblastic, pleomorphic T cells by a modified classification scheme of the Working Formulation. A high level of serum LDH was detected (2.1 times the upper normal limit). Anti HTLV‐1 antibody was also detected in the serum. The atypical lymphocytes were positive for CD3, CD4, CD5, CD7 and HLA‐DR, and negative for CD8. Thus, the clinical, pathologic and immunologic features were those of typical acute‐type ATL.

Cutaneous B-cell lymphoma consisting of large cleaved cells with multilobated nuclei

A 65‐year‐old man was seen at the Ushioda Hospital in Au‐gust 1989, because of a 1‐month history of a tumor on the scalp. The tumor was excised and the diagnosis was malig‐nant lymphoma. The patient was then referred to our de‐partment in September 1989. Several nut‐sized lymph nodes wvepa‐b and m‐fepa for 2 months. Since then, the patient has been free of disease up to the time of writing, July 1992, a period of 2.5 years.